2003
Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation
Haga M, Yamashita A, Paszkowiak J, Sumpio BE, Dardik A. Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation. Journal Of Vascular Surgery 2003, 37: 1277-1284. PMID: 12764276, DOI: 10.1016/s0741-5214(03)00329-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Physiological PhenomenaDisease Models, AnimalHemodynamicsIn Vitro TechniquesMyocytes, Smooth MuscleOscillometryPhosphatidylinositol 3-KinasesPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthSignal TransductionStress, PhysiologicalVascular DiseasesConceptsSignal transduction pathwaysAkt phosphorylationTransduction pathwaysPI3K-Akt signal transduction pathwaySmooth muscle cell proliferationMuscle cell proliferationCell proliferationControl cellsOscillatory shear stressMaximal phosphorylationPI3K inhibitorsCell numberSurvival pathwaysAkt activationBovine aortic SMCSMC survivalInhibitor LY294002Akt pathwayPhosphorylationWestern blot techniqueControl survivalDNA synthesisK inhibitorsAortic SMCsPathwayInvolvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignment
2002
Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols
Araim O, a J, Waterhouse AL, Sumpio BE. Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols. Journal Of Vascular Surgery 2002, 35: 1226-1232. PMID: 12042735, DOI: 10.1067/mva.2002.124358.Peer-Reviewed Original ResearchConceptsRed wine polyphenol extractVascular SMC proliferationRed wine polyphenolsVascular smooth muscle cell proliferationSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationAtherosclerotic diseaseBeneficial effectsWine polyphenolsVascular SMCsTrypan blue exclusion studiesDealcoholized red wineRed wine consumptionCell proliferationPolyphenol extractDose-dependent fashionInhibits SMC proliferationPotential beneficial effectsObserved beneficial effectsBovine aortic SMCDehydrogenase cytotoxicityAortic SMCsInhibitory effectRed wine
1997
Effect of pressure on cultured smooth muscle cells
Watase M, Awolesi M, Ricotta J, Sumpio B. Effect of pressure on cultured smooth muscle cells. Life Sciences 1997, 61: 987-996. PMID: 9296337, DOI: 10.1016/s0024-3205(97)00603-6.Peer-Reviewed Original ResearchEndothelial Cell Effect on Smooth Muscle Cell Collagen Synthesis
Powell R, Hydowski J, Frank O, Bhargava J, Sumpio B. Endothelial Cell Effect on Smooth Muscle Cell Collagen Synthesis. Journal Of Surgical Research 1997, 69: 113-118. PMID: 9202656, DOI: 10.1006/jsre.1997.5045.Peer-Reviewed Original ResearchConceptsGene expressionSMC gene expressionExtracellular matrixEndothelial cellsOpposite endothelial cellsEC-SMC interactionsCell lysate fractionTrichloroacetic acid-precipitable countsCollagen synthesis regulationSynthesis regulationSMC DNANorthern analysisBovine aortic SMCEndothelial cell effectsLysate fractionAcid-precipitable countsType IAortic SMCsCollagen synthesisMatrigelSubstrate compositionCulture substrateType I collagenFibronectinExpressionStrain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways
Mills I, Cohen C, Kamal K, Li G, Shin T, Du W, Sumpio B. Strain activation of bovine aortic smooth muscle cell proliferation and alignment: Study of strain dependency and the role of protein kinase A and C signaling pathways. Journal Of Cellular Physiology 1997, 170: 228-234. PMID: 9066778, DOI: 10.1002/(sici)1097-4652(199703)170:3<228::aid-jcp2>3.0.co;2-q.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2Adenylyl CyclasesAnimalsAortaCattleCell DivisionCells, CulturedCyclic AMPCyclic AMP Response Element-Binding ProteinCyclic AMP-Dependent Protein KinasesEndothelium, VascularLeucine ZippersMuscle, Smooth, VascularProtein Kinase CSignal TransductionStress, MechanicalTranscription FactorsConceptsBovine aortic SMCSMC proliferationCAMP response elementAortic SMCsAC/cAMP/PKAAortic smooth muscle cell proliferationSmooth muscle cell proliferationProtein levelsCyclic AMP accumulationMuscle cell proliferationSmooth muscle cell phenotypeExposure of SMCAdenylate cyclase activityMuscle cell phenotypeAMP accumulationInhibition of PKACAMP/PKACyclic AMP pathwayMultifactorial natureProtein kinase C activityCyclase activityProtein kinase A (PKA) activityLack of involvementEnhanced proliferationCell proliferation