Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration
Freed D, Khan A, Horanyi P, Cafiso D. Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration. Biochemistry 2011, 50: 8792-8803. PMID: 21894979, PMCID: PMC3199607, DOI: 10.1021/bi200971x.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsBinding SitesCrystallography, X-RayElectron Spin Resonance SpectroscopyEscherichia coliEscherichia coli ProteinsLipid BilayersMembrane ProteinsMembrane Transport ProteinsModels, MolecularMutagenesisProtein ConformationProtein Structure, SecondaryProtein Structure, TertiarySolventsSpin LabelsConceptsLine shapeLong-range distance restraintsEPR spectraResonance line shapeΒ-barrel membrane proteinsDifferent rotameric statesFast rotationRotameric statesElectron paramagnetic resonance spectroscopyEPR line shapeParamagnetic resonance spectroscopyMulticomponent spectraElectron paramagnetic resonance line shapesSpectraWeak interactionsProtein-lipid interfaceAtomic resolution structuresDistance restraintsBilayer thicknessLipid environmentLipid bilayersStructure refinementMembrane-facing surfaceProtein structure refinementResonance spectroscopy