2020
Claudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions
Liu F, Xu T, Peng S, Adelman RA, Rizzolo LJ. Claudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions. Experimental Eye Research 2020, 198: 108157. PMID: 32712183, DOI: 10.1016/j.exer.2020.108157.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternCells, CulturedClaudinsGene ExpressionHumansMembrane ProteinsRetinal Pigment EpitheliumRNA, MessengerTight JunctionsUnstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits
Chen X, Singh D, Adelman RA, Rizzolo LJ. Unstimulated, Serum-free Cultures of Retinal Pigment Epithelium Excrete Large Mounds of Drusen-like Deposits. Current Eye Research 2020, 45: 1390-1394. PMID: 32202447, DOI: 10.1080/02713683.2020.1740744.Peer-Reviewed Original ResearchMeSH KeywordsActinsApolipoproteins ECalciumCell Culture TechniquesCell LineCell ProliferationCoculture TechniquesCulture Media, Serum-FreeElectric ImpedanceHumansInduced Pluripotent Stem CellsLipid MetabolismRetinal DrusenRetinal Pigment EpitheliumStem CellsTight JunctionsTissue Inhibitor of Metalloproteinase-3VitronectinConceptsRetinal pigment epithelium
2019
Interactions of the choroid, Bruch's membrane, retinal pigment epithelium, and neurosensory retina collaborate to form the outer blood-retinal-barrier
Fields M, Del Priore LV, Adelman RA, Rizzolo LJ. Interactions of the choroid, Bruch's membrane, retinal pigment epithelium, and neurosensory retina collaborate to form the outer blood-retinal-barrier. Progress In Retinal And Eye Research 2019, 76: 100803. PMID: 31704339, DOI: 10.1016/j.preteyeres.2019.100803.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsDisease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function
Wang SB, Xu T, Peng S, Singh D, Ghiassi-Nejad M, Adelman RA, Rizzolo LJ. Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function. Communications Biology 2019, 2: 113. PMID: 30937396, PMCID: PMC6433901, DOI: 10.1038/s42003-019-0355-0.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumClaudin-19Retinal neurogenesisP1 waveOuter nuclear layerRPE signature genesARPE19 cell lineOcular involvementKidney diseaseVisual functionFamilial hypomagnesaemiaNuclear layerBipolar cellsNewborn miceOcular diseasesPigment epitheliumRetinal isomeraseDiseaseMiceHuman induced pluripotent cellsRetinal differentiationSignature genesCell linesNeurogenesisInduced pluripotent cells
2017
Novel therapeutics for Stargardt disease
Lu LJ, Liu J, Adelman RA. Novel therapeutics for Stargardt disease. Graefe's Archive For Clinical And Experimental Ophthalmology 2017, 255: 1057-1062. PMID: 28285324, DOI: 10.1007/s00417-017-3619-8.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsFenretinideGenetic TherapyHumansLigandsMacular DegenerationPiperidinesRetinal Pigment EpitheliumStargardt DiseaseStem Cell TransplantationConceptsStargardt diseaseVision lossABCA4 geneStem cell transplantationSevere vision lossPromising treatment candidateNovel therapeutic strategiesRetinal pigment epitheliumStem cell therapyVisual acuityStandard treatmentMacular degenerationTreatment candidatesHESC-RPE cellsStargardt patientsTherapeutic strategiesPharmacological agentsPigment epitheliumMacular dystrophyNovel therapeuticsPatientsDiseaseVisual cyclePrevalent formSecond decade
2016
Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression
Peng S, Wang SB, Singh D, Zhao PY, Davis K, Chen B, Adelman RA, Rizzolo LJ. Claudin-3 and claudin-19 partially restore native phenotype to ARPE-19 cells via effects on tight junctions and gene expression. Experimental Eye Research 2016, 151: 179-189. PMID: 27593915, DOI: 10.1016/j.exer.2016.08.021.Peer-Reviewed Original Research
2015
TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE
Zhao PY, Gan G, Peng S, Wang SB, Chen B, Adelman RA, Rizzolo LJ. TRP Channels Localize to Subdomains of the Apical Plasma Membrane in Human Fetal Retinal Pigment EpitheliumTRP Channels of Human Fetal RPE. Investigative Ophthalmology & Visual Science 2015, 56: 1916-1923. PMID: 25736794, PMCID: PMC4364639, DOI: 10.1167/iovs.14-15738.Peer-Reviewed Original ResearchConceptsHuman fetal RPETRP channelsApical membraneFetal RPEApical plasma membraneCell-cell contactTransepithelial electrical resistanceTransient receptor potential channelsTight junctionsSubcellular localizationInhibitor of calpainPrimary ciliaRT-PCRPlasma membraneBasal channel activityQuantitative RT-PCRApical tight junctionsExpression of TRPC4Ion channelsBasolateral surfaceApical microvilliApical surfaceChannel activityConfocal microscopyTRPM3
2013
Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis
Peng S, Gan G, Qiu C, Zhong M, An H, Adelman RA, Rizzolo LJ. Engineering a Blood-Retinal Barrier With Human Embryonic Stem Cell-Derived Retinal Pigment Epithelium: Transcriptome and Functional Analysis. Stem Cells Translational Medicine 2013, 2: 534-544. PMID: 23734062, PMCID: PMC3697821, DOI: 10.5966/sctm.2012-0134.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumBlood-retinal barrierHuman retinal pigment epitheliumPigment epitheliumHuman fetal retinal pigment epitheliumOuter blood-retinal barrierTight junctionsFetal retinal pigment epitheliumEmbryonic stem cell-derived retinal pigment epitheliumAdult retinal pigment epitheliumQuantitative reverse transcription polymerase chain reactionMaturation of hESCReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionHuman embryonic stem cell-derived retinal pigment epitheliumStem cell-derived retinal pigment epitheliumRPE replacement therapyPanel of genesReplacement therapyAnimal modelsHuman embryonic stem cellsRetinal degenerationRPE functionSerum-free mediumHuman retina
2012
Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions
Peng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ. Effects of Proinflammatory Cytokines on the Claudin-19 Rich Tight Junctions of Human Retinal Pigment EpitheliumCytokine Effects on RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2012, 53: 5016-5028. PMID: 22761260, PMCID: PMC3410691, DOI: 10.1167/iovs.11-8311.Peer-Reviewed Original ResearchConceptsHuman fetal RPEEffects of TNFαTransepithelial electrical resistanceProinflammatory cytokinesClaudin-19Tight junctionsAge-related macular degenerationZO-1Effects of cytokinesApical sideSubclinical inflammationTumor necrosisMacular degenerationTNFα receptorsOcular diseasesTNFαSerum-free mediumClaudin-3RPE tight junctionsFetal RPECytokinesClaudin-2Confocal immunofluorescence microscopyInhibitor of apoptosis
2011
Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium
Peng S, Rao VS, Adelman RA, Rizzolo LJ. Claudin-19 and the Barrier Properties of the Human Retinal Pigment Epithelium. Investigative Ophthalmology & Visual Science 2011, 52: 1392-1403. PMID: 21071746, PMCID: PMC3101667, DOI: 10.1167/iovs.10-5984.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsCells, CulturedClaudinsElectric ImpedanceFluorescent Antibody Technique, IndirectGene SilencingGestational AgeHumansImmunoblottingMembrane ProteinsOccludinPermeabilityPolyethylene GlycolsRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingTight JunctionsConceptsRetinal pigment epitheliumTransepithelial electrical resistanceHuman retinal pigment epitheliumHuman fetal RPECultured human fetal retinal pigment epitheliumPigment epitheliumClaudin-19Retinal sideHuman fetal retinal pigment epitheliumSpread of edemaFetal retinal pigment epitheliumFetal human retinal pigment epitheliumEffect of serumTight junctionsRPE permeabilityQuantitative RT-PCRChoroidal capillariesSubpopulation of cellsClaudin-1RPE barrierClaudin-3RPE tight junctionsRT-PCREpitheliumSerum
2010
Inverted Internal Limiting Membrane Flap Technique for Large Macular Holes
Michalewska Z, Michalewski J, Adelman RA, Nawrocki J. Inverted Internal Limiting Membrane Flap Technique for Large Macular Holes. Ophthalmology 2010, 117: 2018-2025. PMID: 20541263, DOI: 10.1016/j.ophtha.2010.02.011.Peer-Reviewed Original ResearchConceptsLarge macular holesILM flap techniqueMacular hole closureMembrane flap techniqueMacular holeGroup 2Group 1Visual acuityFlap techniqueOptical coherence tomographySpectral optical coherence tomographyAnatomic outcomesHole closureStandard macular hole surgeryInternal Limiting Membrane Flap TechniqueCoherence tomographyVisual acuity 12 monthsPars plana vitrectomyMacular hole surgeryFluid-air exchangeLimited visual acuityRetinal pigment epitheliumSurgical failureFoveal anatomyPlana vitrectomy
2009
Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions
Peng S, Adelman RA, Rizzolo LJ. Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2009, 51: 3216-3225. PMID: 20042644, PMCID: PMC2891474, DOI: 10.1167/iovs.09-4162.Peer-Reviewed Original ResearchAngiogenesis InhibitorsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedBevacizumabBlood-Retinal BarrierCapillary PermeabilityCells, CulturedClaudinsElectric ImpedanceEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoblottingPolyethylene GlycolsPotassiumRanibizumabRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumTight JunctionsVascular Endothelial Growth Factor A