2004
Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane
Vessey JP, Shi C, Jollimore CA, Stevens KT, Coca-Prados M, Barnes S, Kelly ME. Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane. Biochemistry And Cell Biology 2004, 82: 708-718. PMID: 15674438, DOI: 10.1139/o04-107.Peer-Reviewed Original ResearchConceptsPlasma membraneHyposmotic stimulationFluorescent membrane dye FM1-43Membrane dye FM1-43Trafficking of channelsClC-3 channelsCiliary epithelial cellsPhosphoinositide-3 kinase inhibitor wortmanninRate of exocytosisClC-3 Cl(-) channelsEpithelial cellsVesicular traffickingDye FM1-43Membrane dynamicsNonpigmented ciliary epithelial cellsInhibitor wortmanninClC-3 antisenseImmunofluorescence microscopyTraffickingClC-3Regulatory volume decreaseFM1-43Cl- channelsMembraneCell swellingFunctional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells
Al-Nakkash L, Iserovich P, Coca-Prados M, Yang H, Reinach P. Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells. The Journal Of Membrane Biology 2004, 201: 41-49. PMID: 15635811, DOI: 10.1007/s00232-004-0706-5.Peer-Reviewed Original ResearchConceptsCorneal epithelial cellsRabbit corneal epithelial cellsVolume-regulated anion channelsEpithelial cellsVoltage-dependent inhibitionHypotonic challengeVolume-activated chloride channelsRegulatory volume decrease (RVD) responseInactive phorbol ester analoguePresence of DIDSExtracellular calciumChloride currentsNiflumic acidRVD responseCell swellingDecrease responseNorthern blot analysisBlot analysisPhorbol dibutyratePhorbol ester analogsChloride channelsVACCNPPBMOsm mediumSV40-immortalized rabbit corneal epithelial cells
2003
A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway
Shi C, Szczesniak A, Mao L, Jollimore C, Coca‐Prados M, Hung O, Kelly ME. A3 adenosine and CB1 receptors activate a PKC‐sensitive Cl− current in human nonpigmented ciliary epithelial cells via a Gβγ‐coupled MAPK signaling pathway. British Journal Of Pharmacology 2003, 139: 475-486. PMID: 12788807, PMCID: PMC1573867, DOI: 10.1038/sj.bjp.0705266.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TransformedChloride ChannelsCiliaDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsEpithelial CellsGTP-Binding Protein beta SubunitsGTP-Binding Protein gamma SubunitsHumansMAP Kinase Signaling SystemProtein Kinase CReceptor, Adenosine A3Receptor, Cannabinoid, CB1ConceptsCB1 receptorsCl- currentSR 141716Receptor agonistNPCE cellsA3 adenosineWhole-cell patch-clamp recordingsCB1 receptor inverse agonistBasal Cl(-) currentCB1 receptor agonistA3 receptor agonistPatch-clamp recordingsHuman CB1 receptorReceptor-transfected cellsReceptor inverse agonistHuman nonpigmented ciliary epithelial cellsSignaling pathwaysAdenosine receptor antagonistNonpigmented ciliary epithelial cellsCiliary epithelial cell linePI3K inhibitorsMitogen-activated protein kinase kinase inhibitorBeta-adrenergic receptor kinaseProtein kinase kinase inhibitorEpithelial cell line
2002
Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line.
Shi C, Barnes S, Coca-Prados M, Kelly ME. Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line. Investigative Ophthalmology & Visual Science 2002, 43: 1525-32. PMID: 11980870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TransformedChloride ChannelsCiliary BodyEnzyme ActivationEnzyme InhibitorsEpithelial CellsPatch-Clamp TechniquesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphoprotein PhosphatasesPhosphorylationProtein-Tyrosine KinasesRabbitsSignal TransductionConceptsProtein tyrosine kinasesProtein tyrosine kinase inhibitorTyrosine kinaseOkadaic acidPP-1Ciliary epithelial cellsHyposmotic stimulationC-Src tyrosine kinasePI3KProtein phosphatase inhibitorPKC inhibitorEpithelial cellsSrc tyrosine kinaseCiliary epithelial cell linePhosphatidylinositol-3 kinase inhibitorKinase inhibitorsProtein phosphatasePhosphatase inhibitorCl- currentEpithelial cell lineNonpigmented ciliary epithelial cellsCell swellingKinasePKC activatorTyrosine kinase inhibitors
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
2000
Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2000, 279: c440-c451. PMID: 10913011, DOI: 10.1152/ajpcell.2000.279.2.c440.Peer-Reviewed Original ResearchTamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells
Mitchell C, Peterson‐Yantorno K, Coca‐Prados M, Civan M. Tamoxifen and ATP synergistically activate Cl− release by cultured bovine pigmented ciliary epithelial cells. The Journal Of Physiology 2000, 525: 183-193. PMID: 10811736, PMCID: PMC2269939, DOI: 10.1111/j.1469-7793.2000.00183.x.Peer-Reviewed Original Research
1999
Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells
Shi C, Ryan J, French A, Coca‐Prados M, Kelly M. Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells. The Journal Of Physiology 1999, 521: 57-67. PMID: 10562334, PMCID: PMC2269649, DOI: 10.1111/j.1469-7793.1999.00057.x.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsCalciumCell LineChloride ChannelsCiliary BodyEnzyme InhibitorsEpithelial CellsNiflumic AcidOsmotic PressurePatch-Clamp TechniquesPhosphorylationProtein Kinase InhibitorsRabbitsSecond Messenger SystemsStilbenesConceptsNon-pigmented ciliary epithelial cellsWhole-cell currentsCiliary epithelial cellsWhole-cell patch-clamp recording techniquePatch-clamp recording techniquesEpithelial cellsTime courseSlower time courseDisulfonic acidSmall conductanceWhole-cell conductanceCl- current amplitudeExposure of cellsVoltage-sensitive blockCl- currentVoltage-independent blockNiflumic acidRecording techniquesDepolarized potentialsCurrent activationAmplitude of currentsOutward rectificationCell swellingNPCE cellsHyposmotic shockA3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells
Mitchell C, Peterson-Yantorno K, Carré D, McGlinn A, Coca-Prados M, Stone R, Civan M. A3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1999, 276: c659-c666. PMID: 10069993, DOI: 10.1152/ajpcell.1999.276.3.c659.Peer-Reviewed Original ResearchConceptsAgonist N6HCE cellsIB-MECAA3 receptorsAdenosine receptorsCiliary processesA2A agonist CGS 21680Rabbit ciliary processesCiliary epitheliumA1-selective agonist N6Specific adenosine receptorsAgonist CGS 21680NPE cellsCl- channelsNonpigmented ciliary epithelial cellsA3 adenosine receptorHuman NPE cellsCGS 21680Ciliary epithelial cellsReceptor subtypesIntracellular Ca2MicroM adenosineRabbit ciliary epitheliumRT-PCREpithelial cellsEffects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1997
Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1997, 273: c1354-c1361. PMID: 9357781, DOI: 10.1152/ajpcell.1997.273.4.c1354.Peer-Reviewed Original ResearchConceptsCiliary epithelial cellsNonpigmented ciliary epithelial cellsWhole-cell currentsEpithelial cellsAdenosine receptorsNPE cellsA2 adenosine receptorsMultiple purinergic receptorsCl- channel blockerHuman NPE cellsIntraocular pressureAdenosine actionPurinergic receptorsHCE cellsRabbit ciliary epitheliumCiliary epitheliumNiflumic acidBovine cellsReceptors
1996
Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells
Coca-Prados M, Sánchez-Torres J, Peterson-Yantorno K, Civan M. Association of ClC-3 Channel with Cl− Transport by Human Nonpigmented Ciliary Epithelial Cells. The Journal Of Membrane Biology 1996, 150: 197-208. PMID: 8661780, DOI: 10.1007/s002329900044.Peer-Reviewed Original Research
1995
PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln
Coca-Prados M, Anguita J, Chalfant M, Civan M. PKC-sensitive Cl- channels associated with ciliary epithelial homologue of pICln. American Journal Of Physiology 1995, 268: c572-c579. PMID: 7534980, DOI: 10.1152/ajpcell.1995.268.3.c572.Peer-Reviewed Original ResearchMeSH KeywordsAlkaloidsAnimalsBase SequenceBlotting, NorthernCells, CulturedChild, PreschoolChloride ChannelsCiliaCystic Fibrosis Transmembrane Conductance RegulatorElectric ConductivityEpitheliumHumansIon ChannelsKineticsMembrane ProteinsMolecular Sequence DataPolymerase Chain ReactionProtein Kinase CRatsRNA, MessengerStaurosporine
1994
Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells.
Civan M, Coca-Prados M, Peterson-Yantorno K. Pathways signaling the regulatory volume decrease of cultured nonpigmented ciliary epithelial cells. Investigative Ophthalmology & Visual Science 1994, 35: 2876-86. PMID: 8188483.Peer-Reviewed Original ResearchConceptsRegulatory volume decreaseNonpigmented ciliary epithelial cellsProtein phosphatase inhibitor okadaic acidCiliary epithelial cellsPhosphatase inhibitor okadaic acidCultured nonpigmented ciliary epithelial cellsInhibitor okadaic acidEpithelial cellsNPE cellsHuman nonpigmented ciliary epithelial cellsCl- channelsCyclic AMPPKC inhibitor staurosporineEndogenous PKC activityOkadaic acidRegulatory responsesIntracellular cAMP concentrationCell typesPKC activatorCalcium-calmodulinPKC activityCell sortingElectronic cell sortingPathwayStaurosporine