2004
Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells
Al-Nakkash L, Iserovich P, Coca-Prados M, Yang H, Reinach P. Functional and Molecular Characterization of a Volume-activated Chloride Channel in Rabbit Corneal Epithelial Cells. The Journal Of Membrane Biology 2004, 201: 41-49. PMID: 15635811, DOI: 10.1007/s00232-004-0706-5.Peer-Reviewed Original ResearchConceptsCorneal epithelial cellsRabbit corneal epithelial cellsVolume-regulated anion channelsEpithelial cellsVoltage-dependent inhibitionHypotonic challengeVolume-activated chloride channelsRegulatory volume decrease (RVD) responseInactive phorbol ester analoguePresence of DIDSExtracellular calciumChloride currentsNiflumic acidRVD responseCell swellingDecrease responseNorthern blot analysisBlot analysisPhorbol dibutyratePhorbol ester analogsChloride channelsVACCNPPBMOsm mediumSV40-immortalized rabbit corneal epithelial cells
1998
Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume
Reeves R, Sanchez-Torres J, Coca-Prados M, Cammarata P. Expression of pICln mRNA in cultured bovine lens epithelial cells: response to changes in cell volume. Current Eye Research 1998, 17: 861-869. PMID: 9746432, DOI: 10.1076/ceyr.17.9.861.5140.Peer-Reviewed Original ResearchConceptsBovine lens epithelial cellsCultured bovine lens epithelial cellsLens epithelial cellsHypertonic mediumChannel regulatory proteinRapid cellular swellingEpithelial cellsNorthern blot analysisTranscriptional regulationMyo-inositol effluxRegulatory proteinsMRNA expressionAnion channelCellular shrinkageCell volume changesCell volumeBlot analysisCell volume reductionExpression levelsMRNAChloride movementExpressionCellsMedium conditionsCellular swelling
1997
The prostaglandin transporter is widely expressed in ocular tissues
Schuster V, Lu R, Coca-Prados M. The prostaglandin transporter is widely expressed in ocular tissues. Survey Of Ophthalmology 1997, 41: s41-s45. PMID: 9154275, DOI: 10.1016/s0039-6257(97)80006-9.Peer-Reviewed Original Research
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1992
Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium
Coca-Prados M, Ghosh S, Gilula N, Kumar N. Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium. Current Eye Research 1992, 11: 113-122. PMID: 1374005, DOI: 10.3109/02713689209000061.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsGene productsCiliary epithelial cellsApical plasma membrane domainPlasma membrane domainsEpithelial cellsAlpha 3Plasma membrane regionGap junction genesPE cellsNorthern blot analysisGap junction proteinImmunofluorescence localization studiesAlpha 1 transcriptsIndirect immunofluorescence localization studiesIntact tissueMembrane domainsAlpha 1 mRNABeta 1Predominant transcriptGap junction mRNAMembrane regionsJunction genesLocalization studiesCell types