1992
Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium
Coca-Prados M, Ghosh S, Gilula N, Kumar N. Expression and cellular distribution of the al gap junction gene product in the ocular pigmented ciliary epithelium. Current Eye Research 1992, 11: 113-122. PMID: 1374005, DOI: 10.3109/02713689209000061.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsGene productsCiliary epithelial cellsApical plasma membrane domainPlasma membrane domainsEpithelial cellsAlpha 3Plasma membrane regionGap junction genesPE cellsNorthern blot analysisGap junction proteinImmunofluorescence localization studiesAlpha 1 transcriptsIndirect immunofluorescence localization studiesIntact tissueMembrane domainsAlpha 1 mRNABeta 1Predominant transcriptGap junction mRNAMembrane regionsJunction genesLocalization studiesCell types
1990
Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium.
Ghosh S, Freitag A, Martin-Vasallo P, Coca-Prados M. Cellular distribution and differential gene expression of the three alpha subunit isoforms of the Na,K-ATPase in the ocular ciliary epithelium. Journal Of Biological Chemistry 1990, 265: 2935-2940. PMID: 1689295, DOI: 10.1016/s0021-9258(19)39891-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCattleCell MembraneCell Transformation, ViralCiliary BodyFlow CytometryFluorescent Antibody TechniqueGene ExpressionIsoenzymesMacromolecular SubstancesMicroscopy, ElectronPigment Epithelium of EyeRatsRNASimian virus 40Sodium-Potassium-Exchanging ATPaseConceptsAlpha-subunit isoformsAlpha isoformSubunit isoformsK-ATPaseBasolateral plasma membrane domainsK-ATPase alpha subunitPlasma membrane domainsImmunoreactive signalPE cellsDifferential gene expressionAlpha 3Alpha 3 polypeptidesNPE cellsCell linesAlpha 2Alpha 1 polypeptideAlpha 1Pattern of expressionAlpha 3 isoformAlpha 1 isoformMembrane domainsSpecific cDNA probesSimian virus 40Differential immunostaining patternAlpha 2 isoform
1989
Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells
Martin‐Vasallo P, Ghosh S, Coca‐Prados M. Expression of Na,K‐ATPase alpha subunit isoforms in the human ciliary body and cultured ciliary epithelial cells. Journal Of Cellular Physiology 1989, 141: 243-252. PMID: 2553750, DOI: 10.1002/jcp.1041410203.Peer-Reviewed Original ResearchConceptsK-ATPase alpha subunit isoformsAlpha-subunit isoformsSubunit isoformsCultured cellsK-ATPase alpha subunitPE cellsExpression of NaBasolateral membrane domainsNorthern hybridization analysisNPE cell linesAlpha 2 mRNACell linesAlpha 3 isoformMembrane domainsAlpha 3 mRNAAlpha 1Alpha subunitCultured ciliary epithelial cellsWestern blot analysisHybridization analysisIsoformsCiliary epithelial cellsNPE layerBlot analysisK-ATPase