2005
FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner
Berry FB, O'Neill MA, Coca-Prados M, Walter MA. FOXC1 Transcriptional Regulatory Activity Is Impaired by PBX1 in a Filamin A-Mediated Manner. Molecular And Cellular Biology 2005, 25: 1415-1424. PMID: 15684392, PMCID: PMC548007, DOI: 10.1128/mcb.25.4.1415-1424.2005.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationCell NucleusChromobox Protein Homolog 5Chromosomal Proteins, Non-HistoneContractile ProteinsDNA-Binding ProteinsEye AbnormalitiesFilaminsForkhead Transcription FactorsHeLa CellsHeterochromatinHumansMicrofilament ProteinsMutationProtein BindingProto-Oncogene ProteinsTranscription FactorsTumor Cells, CulturedConceptsFilamin AHuman nonpigmented ciliary epithelial cellsFunction mutationsNonpigmented ciliary epithelial cellsAxenfeld-Rieger syndromeAutosomal dominant disorderCiliary epithelial cellsMelanoma cellsSkeletal disordersElevated levelsDominant disorderEpithelial cellsImportant transcription factorSkeletal phenotypeProtein filamin AFOXC1Inhibitory activityDisorders
2004
Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane
Vessey JP, Shi C, Jollimore CA, Stevens KT, Coca-Prados M, Barnes S, Kelly ME. Hyposmotic activation of ICl,swell in rabbit nonpigmented ciliary epithelial cells involves increased ClC-3 trafficking to the plasma membrane. Biochemistry And Cell Biology 2004, 82: 708-718. PMID: 15674438, DOI: 10.1139/o04-107.Peer-Reviewed Original ResearchConceptsPlasma membraneHyposmotic stimulationFluorescent membrane dye FM1-43Membrane dye FM1-43Trafficking of channelsClC-3 channelsCiliary epithelial cellsPhosphoinositide-3 kinase inhibitor wortmanninRate of exocytosisClC-3 Cl(-) channelsEpithelial cellsVesicular traffickingDye FM1-43Membrane dynamicsNonpigmented ciliary epithelial cellsInhibitor wortmanninClC-3 antisenseImmunofluorescence microscopyTraffickingClC-3Regulatory volume decreaseFM1-43Cl- channelsMembraneCell swelling
2003
Sex steroid hormone metabolism takes place in human ocular cells
Coca-Prados M, Ghosh S, Wang Y, Escribano J, Herrala A, Vihko P. Sex steroid hormone metabolism takes place in human ocular cells. The Journal Of Steroid Biochemistry And Molecular Biology 2003, 86: 207-216. PMID: 14568574, DOI: 10.1016/j.jsbmb.2003.08.001.Peer-Reviewed Original ResearchConceptsSex steroid hormone metabolismCiliary epitheliumHuman ocular cellsSex steroid hormonesHuman ciliary epithelial cellsSteroid hormone metabolismAndrogen agonistsCiliary epithelial cellsEstrogenic milieuOcular diseasesOcular cellsSteroid hormonesMRNA expressionImportant mediatorOxidative enzymatic activityHuman ciliary epitheliumGene expressionEpithelial cellsHormone metabolismHormone-responsive genesSecretory epitheliumEpitheliumH time courseCell linesTime courseExpression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma
Liao S, Ivanov S, Ivanova A, Ghosh S, Cote M, Keefe K, Coca-Prados M, Stanbridge E, Lerman M. Expression of cell surface transmembrane carbonic anhydrase genes CA9 and CA12 in the human eye: overexpression of CA12 (CAXII) in glaucoma. Journal Of Medical Genetics 2003, 40: 257. PMID: 12676895, PMCID: PMC1735430, DOI: 10.1136/jmg.40.4.257.Peer-Reviewed Original ResearchConceptsNon-pigmented ciliary epithelial cellsGlaucoma patientsEpithelium of corneaNPE cell linesNPE cellsAqueous humor productionCultured NPE cellsGlaucoma eyesIntraocular pressureAqueous humor secretionGlaucomatous eyesCAIX expressionCiliary epithelial cellsCAXII expressionEye donorsCA9 mRNANormal eyesPlasma membrane proteinsEmbryonic eye developmentFundamental physiological processesGlaucoma donorsGlaucomaCiliary epitheliumCAIXEpithelial cells
2002
Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line.
Shi C, Barnes S, Coca-Prados M, Kelly ME. Protein tyrosine kinase and protein phosphatase signaling pathways regulate volume-sensitive chloride currents in a nonpigmented ciliary epithelial cell line. Investigative Ophthalmology & Visual Science 2002, 43: 1525-32. PMID: 11980870.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TransformedChloride ChannelsCiliary BodyEnzyme ActivationEnzyme InhibitorsEpithelial CellsPatch-Clamp TechniquesPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphoprotein PhosphatasesPhosphorylationProtein-Tyrosine KinasesRabbitsSignal TransductionConceptsProtein tyrosine kinasesProtein tyrosine kinase inhibitorTyrosine kinaseOkadaic acidPP-1Ciliary epithelial cellsHyposmotic stimulationC-Src tyrosine kinasePI3KProtein phosphatase inhibitorPKC inhibitorEpithelial cellsSrc tyrosine kinaseCiliary epithelial cell linePhosphatidylinositol-3 kinase inhibitorKinase inhibitorsProtein phosphatasePhosphatase inhibitorCl- currentEpithelial cell lineNonpigmented ciliary epithelial cellsCell swellingKinasePKC activatorTyrosine kinase inhibitors
2001
PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells
Fleischhauer J, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. PGE2, Ca2+, and cAMP mediate ATP activation of Cl− channels in pigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2001, 281: c1614-c1623. PMID: 11600425, DOI: 10.1152/ajpcell.2001.281.5.c1614.Peer-Reviewed Original ResearchConceptsPigmented ciliary epithelial cellsCiliary epithelial cellsCl- channelsEpithelial cellsPE cellsCl- channel activityAction of nucleotidesProtein kinaseNonpigmented ciliary epithelial cellsSecond messengerCell sortingChannel activityATP activationElectronic cell sortingATPSequential cascadeChannel activationNucleotidesP2Y receptorsPatch clampingATP assayATP releaseCellsMicroM ATPCAMP
2000
Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Similarity of A3-adenosine and swelling-activated Cl− channels in nonpigmented ciliary epithelial cells. American Journal Of Physiology - Cell Physiology 2000, 279: c440-c451. PMID: 10913011, DOI: 10.1152/ajpcell.2000.279.2.c440.Peer-Reviewed Original ResearchNa+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells
Counillon L, Touret N, Bidet M, Peterson-Yantorno K, Coca-Prados M, Stuart-Tilley A, Wilhelm S, Alper S, Civan M. Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells. Pflügers Archiv - European Journal Of Physiology 2000, 440: 667-678. PMID: 11007305, DOI: 10.1007/s004240000302.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAntiportersCattleCell Line, TransformedChloride-Bicarbonate AntiportersCiliary BodyEpithelial CellsImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceMicroscopy, VideoModels, BiologicalPigmentationReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSLC4A ProteinsSodiumSodium-Hydrogen ExchangersConceptsAqueous humor formationCl-/HCO3Epithelial cell layerTranscriptase-polymerase chain reaction amplificationPE cellsCell layerHuman ciliary bodyAE2 Cl-/HCO3Medical therapyNHE-1 isoformCiliary epithelial cellsCiliary bodyAqueous humorCiliary epithelial bilayerPolymerase chain reaction amplificationChain reaction amplificationNHE-1Rabbit ciliary epitheliumCiliary epitheliumEpithelial cellsBovine pigmented ciliary epithelial cellsGap junctionsRegulatory volume increasePigmented ciliary epithelial cellsExternal Cl
1999
Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells
Shi C, Ryan J, French A, Coca‐Prados M, Kelly M. Hyposmotically activated chloride channels in cultured rabbit non‐pigmented ciliary epithelial cells. The Journal Of Physiology 1999, 521: 57-67. PMID: 10562334, PMCID: PMC2269649, DOI: 10.1111/j.1469-7793.1999.00057.x.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsCalciumCell LineChloride ChannelsCiliary BodyEnzyme InhibitorsEpithelial CellsNiflumic AcidOsmotic PressurePatch-Clamp TechniquesPhosphorylationProtein Kinase InhibitorsRabbitsSecond Messenger SystemsStilbenesConceptsNon-pigmented ciliary epithelial cellsWhole-cell currentsCiliary epithelial cellsWhole-cell patch-clamp recording techniquePatch-clamp recording techniquesEpithelial cellsTime courseSlower time courseDisulfonic acidSmall conductanceWhole-cell conductanceCl- current amplitudeExposure of cellsVoltage-sensitive blockCl- currentVoltage-independent blockNiflumic acidRecording techniquesDepolarized potentialsCurrent activationAmplitude of currentsOutward rectificationCell swellingNPCE cellsHyposmotic shockDifferential gene expression in the human ciliary epithelium
Coca-Prados M, Escribano J, Ortego J. Differential gene expression in the human ciliary epithelium. Progress In Retinal And Eye Research 1999, 18: 403-429. PMID: 10192520, DOI: 10.1016/s1350-9462(98)00026-3.Peer-Reviewed Original ResearchConceptsDifferential gene expressionCell-specific expressionDiversity of functionsExpression of genesOcular ciliary bodySubtractive libraryNeuropeptide processing enzymesMolecular basisGene expressionCultured ciliary epithelial cellsCell differentiationCiliary epitheliumHuman ciliary epitheliumAntioxidative enzymesGenesCiliary epithelial cellsRegulatory peptidesLight-dark cycleEpithelial cellsMuscle cellsCiliary muscle cellsAutocrine mechanismExpressionPlasma proteinsParacrine mechanismsFunctional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells
Ortego J, Coca-Prados M. Functional Expression of Components of the Natriuretic Peptide System in Human Ocular Nonpigmented Ciliary Epithelial Cells. Biochemical And Biophysical Research Communications 1999, 258: 21-28. PMID: 10222228, DOI: 10.1006/bbrc.1999.0573.Peer-Reviewed Original ResearchConceptsNatriuretic peptide systemNPR-B receptorNPR-C receptorCiliary epithelial cellsReceptor mRNACiliary epitheliumEpithelial cellsNPE cellsCGMP responseAutocrine/paracrine modulatorPeptide systemHuman ocular ciliary epitheliumNonpigmented ciliary epithelial cellsOcular ciliary epitheliumCultured NPE cellsLong-term downregulationC-ANP4Paracrine modulatorEye pressureBNPActivation of PKCBasal levelsInhibitory effectRT-PCRReceptorsA3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells
Mitchell C, Peterson-Yantorno K, Carré D, McGlinn A, Coca-Prados M, Stone R, Civan M. A3 adenosine receptors regulate Cl−channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1999, 276: c659-c666. PMID: 10069993, DOI: 10.1152/ajpcell.1999.276.3.c659.Peer-Reviewed Original ResearchConceptsAgonist N6HCE cellsIB-MECAA3 receptorsAdenosine receptorsCiliary processesA2A agonist CGS 21680Rabbit ciliary processesCiliary epitheliumA1-selective agonist N6Specific adenosine receptorsAgonist CGS 21680NPE cellsCl- channelsNonpigmented ciliary epithelial cellsA3 adenosine receptorHuman NPE cellsCGS 21680Ciliary epithelial cellsReceptor subtypesIntracellular Ca2MicroM adenosineRabbit ciliary epitheliumRT-PCREpithelial cellsEffects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells
Sánchez-Torres J, Huang W, Civan M, Coca-Prados M. Effects of hypotonic swelling on the cellular distribution and expression of pICln in human nonpigmented ciliary epithelial cells. Current Eye Research 1999, 18: 408-416. PMID: 10435827, DOI: 10.1076/ceyr.18.6.408.5266.Peer-Reviewed Original ResearchConceptsOpen reading frameCiliary epithelial cellsPlasma membraneFusion proteinWestern blot analysisEpithelial cellsCellular distributionTag fusion proteinCandidate gene productsBlot analysisHuman nonpigmented ciliary epithelial cellsTotal cell extractsHypotonic treatmentHuman ciliary epithelial cellsMammalian cellsPolyclonal antibodiesReading frameGene productsNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsPerinuclear regionCellular localizationCell extractsCultured cellsProtein
1997
Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells
Carré D, Mitchell C, Peterson-Yantorno K, Coca-Prados M, Civan M. Adenosine stimulates Cl− channels of nonpigmented ciliary epithelial cells. American Journal Of Physiology 1997, 273: c1354-c1361. PMID: 9357781, DOI: 10.1152/ajpcell.1997.273.4.c1354.Peer-Reviewed Original ResearchConceptsCiliary epithelial cellsNonpigmented ciliary epithelial cellsWhole-cell currentsEpithelial cellsAdenosine receptorsNPE cellsA2 adenosine receptorsMultiple purinergic receptorsCl- channel blockerHuman NPE cellsIntraocular pressureAdenosine actionPurinergic receptorsHCE cellsRabbit ciliary epitheliumCiliary epitheliumNiflumic acidBovine cellsReceptorsGene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1996
Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells
CIVAN M, COCA-PRADOS M, PETERSON-YANTORNO K. Regulatory Volume Increase of Human Non-pigmented Ciliary Epithelial Cells. Experimental Eye Research 1996, 62: 627-640. PMID: 8983945, DOI: 10.1006/exer.1996.0074.Peer-Reviewed Original ResearchConceptsRegulatory volume increaseCiliary epithelial cellsEpithelial cellsNon-pigmented ciliary epithelial cellsPKC inhibitor staurosporineHuman non-pigmented ciliary epithelial cellsElectronic cell sizingRegulatory volume responsesAcid metabolismSame cellsTransport inhibitorsStaurosporineHuman NonSymportIdentification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium
Ortego J, Escribano J, Crabb J, Coca‐Prados M. Identification of a Neuropeptide and Neuropeptide‐Processing Enzymes in Aqueous Humor Confers Neuroendocrine Features to the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1996, 66: 787-796. PMID: 8592153, DOI: 10.1046/j.1471-4159.1996.66020787.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnimalsAqueous HumorBase SequenceCarboxypeptidase HCarboxypeptidasesCell LineChild, PreschoolChromograninsCiliary BodyEpithelial CellsEpitheliumFluorescent Antibody Technique, IndirectHumansMixed Function OxygenasesMolecular Sequence DataMultienzyme ComplexesNeurosecretory SystemsProteinsRatsConceptsNeuropeptide processing enzymesCarboxypeptidase ECiliary epithelial cellsOcular ciliary epitheliumSecretogranin IICell-cell communicationEpithelial cellsHuman ocular ciliary epitheliumCDNA clonesCell signalingNonpigmented ciliary epithelial cellsCultured ciliary epithelial cellsProcessing enzymesMolecular markersPeptidylglycine alphaPhorbol esterSpecialized groupSpecific probesMammalian eyeNeuroendocrine tissuesEnzymeCiliary epitheliumKey functionsCellsPolymerase chain reaction
1995
Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium.
Coca-Prados M, Fernández-Cabezudo M, Sánchez-Torres J, Crabb J, Ghosh S. Cell-specific expression of the human Na+,K(+)-ATPase beta 2 subunit isoform in the nonpigmented ciliary epithelium. Investigative Ophthalmology & Visual Science 1995, 36: 2717-28. PMID: 7499094.Peer-Reviewed Original ResearchConceptsBeta 2 isoformNonpigmented ciliary epithelial cellsOcular tissuesCiliary epitheliumBeta 1NPE cell linesNonpigmented ciliary epitheliumNPE cellsHuman ocular tissuesSubunit isoformsBlot analysisATPase subunit isoformsSpecific anti-peptide antibodiesRestricted cellular distributionPolymerase chain reactionCiliary epithelial cellsBeta-subunit isoformsAlpha-subunit isoformsAnti-peptide antibodiesODM-2Polymerase chain reaction amplificationChain reaction amplificationHuman ciliary epitheliumCell-specific distributionPattern of expressionIsolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1
Escribano J, Ortego J, Coca-Prados M. Isolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1. The Journal Of Biochemistry 1995, 118: 921-931. PMID: 8749308, DOI: 10.1093/jb/118.5.921.Peer-Reviewed Original ResearchConceptsSubtractive cDNA libraryOcular ciliary bodyCDNA clonesCDNA libraryCell-restricted expressionPartial DNA sequencesCell-specific genesPotential candidate genesSubtractive libraryComplement component C4Significant homologyHomology searchProtein databaseDNA sequencesCiliary bodyCandidate genesTranscriptional expressionNorthern hybridizationSignificant genesGenesIntraocular pressureCiliary epithelial cellsPlasma proteinsProteinVascular endothelial cells
1994
Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors.
Hong T, Escribano J, Coca-Prados M. Isolation of cDNA clones encoding the 80-kd subunit protein of the human autoantigen Ku (p70/p80) by antisera raised against ciliary processes of human eye donors. Investigative Ophthalmology & Visual Science 1994, 35: 4023-30. PMID: 7960584.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearAutoantigensBlotting, WesternCell LineCiliary BodyClone CellsDNA HelicasesDNA-Binding ProteinsDNA, ComplementaryFluorescent Antibody TechniqueHumansImmunoglobulin GKu AutoantigenMaleMiceMice, Inbred BALB CMiddle AgedMolecular WeightNuclear ProteinsPigment Epithelium of EyeRNA, MessengerTissue DonorsConceptsHuman eye donorsPolymyositis overlap syndromeScleroderma-polymyositis overlap syndromeODM-2 cellsHuman ciliary processesCiliary processesOverlap syndromeEye donorsCiliary epitheliumSpecies-specific immune responseAnti-Ku antibodiesVariety of antigensCell linesEpithelium cell linePlaque-purified clonesCiliary epithelial cell lineOcular ciliary epitheliumWestern blot analysisSubunit proteinsEpithelial cell lineCiliary epithelial cellsImmune responseOcular tissues