2015
A Renal-Like Organic Anion Transport System in the Ciliary Epithelium of the Bovine and Human Eye
Lee J, Shahidullah M, Hotchkiss A, Coca-Prados M, Delamere NA, Pelis RM. A Renal-Like Organic Anion Transport System in the Ciliary Epithelium of the Bovine and Human Eye. Molecular Pharmacology 2015, 87: 697-705. PMID: 25661037, PMCID: PMC6067639, DOI: 10.1124/mol.114.096578.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological Transport, ActiveCattleCiliary BodyDicarboxylic Acid TransportersHumansKidney CortexMultidrug Resistance-Associated ProteinsOrganic Anion Transport Protein 1Organic Anion TransportersOrganic Anion Transporters, Sodium-DependentOrganic Anion Transporters, Sodium-IndependentRetinal Pigment EpitheliumRNA, MessengerSymportersConceptsCiliary bodyAqueous humorPara-aminohippurateUssing chambersOcular tissuesReverse transcription-polymerase chain reactionOrganic anion transport systemHuman ocular tissuesBovine ciliary bodyHuman ciliary bodyPerfused eyePolymerase chain reactionBasolateral membraneEye preparationsTransporter expressionOrganic anion transportCiliary epitheliumAnion transport systemHuman eyeEpithelial cellsChain reactionBlood sideBlood directionEyesBarrier epithelia
2014
Characterization of Vitamin D Production by Human Ocular Barrier CellsVitamin D Production by Human Ocular Barrier Cells
Alsalem JA, Patel D, Susarla R, Coca-Prados M, Bland R, Walker EA, Rauz S, Wallace GR. Characterization of Vitamin D Production by Human Ocular Barrier CellsVitamin D Production by Human Ocular Barrier Cells. Investigative Ophthalmology & Visual Science 2014, 55: 2140-2147. PMID: 24576880, DOI: 10.1167/iovs.13-13019.Peer-Reviewed Original ResearchConceptsHuman scleral fibroblastsVitamin D3Vitamin D receptorBarrier epithelial cellsPrimary human scleral fibroblastsEnzyme immunoassayD productionUltraviolet B sunlightEpithelial cellsVitamin D productionRetinal pigment epithelial cell lineBarrier cellsExtrarenal synthesisHydroxyvitamin D3Epithelial cell lineImmune regulationCiliary bodyD receptorExpression of componentsSkin precursorsODM-2Scleral fibroblastsBarrier sitesCorneal endothelialBarrier function
2013
MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels
Hotchkiss A, Pasumarthi K, Coca‐Prados M, Pelis R. MRP4 and MRP5 in the ciliary epithelium of the eye and their potential role in regulating extracellular cAMP levels. The FASEB Journal 2013, 27: 732.3-732.3. DOI: 10.1096/fasebj.27.1_supplement.732.3.Peer-Reviewed Original ResearchMultidrug resistance-associated proteinCAMP levelsExtracellular cAMP levelsCiliary epitheliumAqueous humor dynamicsResistance-associated proteinCiliary epithelial cell lineHuman ciliary bodyCE cellsEpithelial cell lineMRP inhibitorsCiliary bodyAdrenergic stimulationIntracellular cAMPIntracellular accumulationPotential roleCell linesIndomethacinFurosemideMRP4Cyclic nucleotidesMRP5EpitheliumIbuprofenCells
2012
Functional Expression of MRP4 in the Ciliary Epithelium of the Eye
Pelis R, Coca-Prados M. Functional Expression of MRP4 in the Ciliary Epithelium of the Eye. The FASEB Journal 2012, 26: 694.10-694.10. DOI: 10.1096/fasebj.26.1_supplement.694.10.Peer-Reviewed Original ResearchFunctional expressionOrganic anion transportCiliary epitheliumAnion transportPara-aminohippurateMRP4 protein levelsCiliary body epithelial cellsHuman ciliary epitheliumCultured cellsFunctional assaysProtein 4Dose-dependent mannerProtein levelsCell linesEfflux pumpsHuman ciliary bodyUseful systemSimian virusEpithelial cellsWestern blottingIntracellular accumulationCiliary bodyMrp4 mRNAMRP4 expressionExpression
2009
Localization of Multidrug Resistance-Associated Protein 2 in the Nonpigmented Ciliary Epithelium of the Eye
Pelis RM, Shahidullah M, Ghosh S, Coca-Prados M, Wright SH, Delamere NA. Localization of Multidrug Resistance-Associated Protein 2 in the Nonpigmented Ciliary Epithelium of the Eye. Journal Of Pharmacology And Experimental Therapeutics 2009, 329: 479-485. PMID: 19201990, PMCID: PMC2672870, DOI: 10.1124/jpet.108.149625.Peer-Reviewed Original ResearchConceptsNonpigmented epitheliumMicroM MK571Ciliary bodyMultidrug resistance associated protein 2Therapeutic drugsMrp2 proteinIntracellular accumulationBlood-aqueous barrierNonpigmented ciliary epitheliumProtein 2Human ciliary bodyMicroM indomethacinMRP inhibitorsIntraocular tissuesAqueous humorApical membraneMRP2 mRNAWestern blotMultidrug resistanceCiliary epitheliumMicroM cyclosporinPorcine eyesMRP2Cell layerNative human
2007
New perspectives in aqueous humor secretion and in glaucoma: The ciliary body as a multifunctional neuroendocrine gland
Coca-Prados M, Escribano J. New perspectives in aqueous humor secretion and in glaucoma: The ciliary body as a multifunctional neuroendocrine gland. Progress In Retinal And Eye Research 2007, 26: 239-262. PMID: 17321191, DOI: 10.1016/j.preteyeres.2007.01.002.Peer-Reviewed Original ResearchConceptsIntraocular pressureCiliary bodyAqueous humor secretionAqueous humorCiliary epitheliumGlaucoma-associated genesImmune privilege statusDevelopment of glaucomaGlutamate-metabolizing enzymesPathophysiology of glaucomaAnti-angiogenic factorsSteroid-converting enzymesDiurnal circadian rhythmCiliary blood flowOcular ciliary bodyAnterior segmentBlood flowMultifactorial eventGlaucomaNeuroendocrine systemOutflow pathwayEndocrine communicationEndocrine systemEndocrine peptidesRegulatory peptidesMolecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body
Bertazolli-Filho R, Coca-Prados M, Haddad A, Laicine EM. Molecular Analysis of Neurolysin Expression in the Rat and Bovine Ciliary Body. Current Eye Research 2007, 32: 751-756. PMID: 17882707, DOI: 10.1080/02713680701573381.Peer-Reviewed Original Research
2002
Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium
Cullinane A, Leung P, Ortego J, Coca-Prados M, Harvey B. Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium. British Journal Of Ophthalmology 2002, 86: 676. PMID: 12034692, PMCID: PMC1771149, DOI: 10.1136/bjo.86.6.676.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IICalciumCell SizeCells, CulturedCiliary BodyElectrophysiologyHumansLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPigment Epithelium of EyePotassium Channels, Calcium-ActivatedReceptors, AngiotensinRenin-Angiotensin SystemReverse Transcriptase Polymerase Chain ReactionSignal TransductionConceptsHNPE cellsAngiotensin IINon-pigmented epitheliumSecretory functionAddition of AIIRenin-angiotensin system componentsRenin-angiotensin system expressionIon channel activityPotassium ion channel activityActive BK channelsExpression of angiotensinogenAqueous humor dynamicsTreatment of glaucomaChannel activitySingle-channel patch-clamp techniquesPatch-clamp techniqueBK channel activationLarge-conductance potassium channelsHuman ciliary bodyPolymerase chain reaction analysisReceptor antagonistCurrent armamentariumCiliary bodyChain reaction analysisRAS expression
2000
Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells
Counillon L, Touret N, Bidet M, Peterson-Yantorno K, Coca-Prados M, Stuart-Tilley A, Wilhelm S, Alper S, Civan M. Na+/H+ and Cl–/HCO3–-antiporters of bovine pigmented ciliary epithelial cells. Pflügers Archiv - European Journal Of Physiology 2000, 440: 667-678. PMID: 11007305, DOI: 10.1007/s004240000302.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnion Transport ProteinsAntiportersCattleCell Line, TransformedChloride-Bicarbonate AntiportersCiliary BodyEpithelial CellsImmunohistochemistryMembrane ProteinsMicroscopy, FluorescenceMicroscopy, VideoModels, BiologicalPigmentationReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSLC4A ProteinsSodiumSodium-Hydrogen ExchangersConceptsAqueous humor formationCl-/HCO3Epithelial cell layerTranscriptase-polymerase chain reaction amplificationPE cellsCell layerHuman ciliary bodyAE2 Cl-/HCO3Medical therapyNHE-1 isoformCiliary epithelial cellsCiliary bodyAqueous humorCiliary epithelial bilayerPolymerase chain reaction amplificationChain reaction amplificationNHE-1Rabbit ciliary epitheliumCiliary epitheliumEpithelial cellsBovine pigmented ciliary epithelial cellsGap junctionsRegulatory volume increasePigmented ciliary epithelial cellsExternal Cl
1997
Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells
ORTEGO J, ESCRIBANO J, COCA-PRADOS M. Gene Expression of Proteases and Protease Inhibitors in the Human Ciliary Epithelium and ODM-2 Cells. Experimental Eye Research 1997, 65: 289-299. PMID: 9268597, DOI: 10.1006/exer.1997.0333.Peer-Reviewed Original ResearchMeSH KeywordsAgedAlpha 1-AntitrypsinAlpha-MacroglobulinsBlotting, NorthernBlotting, WesternCarcinogensCathepsin DCathepsin KCathepsinsCell LineCiliary BodyCysteine EndopeptidasesDNA, ComplementaryEndopeptidasesEpithelial CellsGene ExpressionGlycoproteinsHumansMiddle AgedPolymerase Chain ReactionPrecipitin TestsPregnancy ProteinsProtease InhibitorsSerine Proteinase InhibitorsTetradecanoylphorbol AcetateConceptsODM-2 cellsPP5/TFPICiliary epithelial cellsComplementary DNARegulation of transcriptionProteinase inhibitorsEpithelial cellsNon-protein kinase CCathepsin DProtein kinase C activationKinase C activationPattern of expressionCiliary bodyImmunoprecipitation experimentsGene expressionNorthern analysisCultured ciliary epithelial cellsKinase CWestern blot analysisHuman ciliary epitheliumAlpha-phorbol didecanoatePhorbol esterC activationBlot analysisHuman proteinases
1996
Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor.
Ortego J, Escribano J, Becerra S, Coca-Prados M. Gene expression of the neurotrophic pigment epithelium-derived factor in the human ciliary epithelium. Synthesis and secretion into the aqueous humor. Investigative Ophthalmology & Visual Science 1996, 37: 2759-67. PMID: 8977492.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAqueous HumorBlotting, NorthernCattleCell LineCells, CulturedChromatography, AffinityCiliary BodyDNA PrimersEye ProteinsFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoenzyme TechniquesNerve Growth FactorsPigment Epithelium of EyePolymerase Chain ReactionProtein BiosynthesisProteinsRabbitsSerpinsTranscription, GeneticConceptsPigment epithelium-derived factorEpithelium-derived factorPolymerase chain reactionCiliary epitheliumCiliary bodyOcular tissuesBlot analysisHuman ciliary epitheliumNeuronal survival activityHuman ocular ciliary epitheliumRetinal pigment epitheliumNorthern blot analysisCell linesBovine ocular tissuesPars plicata regionOcular ciliary bodyOcular ciliary epitheliumWestern blot analysisFactor antibodyPEDF expressionTrophic factorsPigment epitheliumPEDF proteinPEDF transcriptAqueous humor
1995
Isolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1
Escribano J, Ortego J, Coca-Prados M. Isolation and Characterization of Cell-Specific cDNA Clones from a Subtractive Library of the Ocular Ciliary Body of a Single Normal Human Donor: Transcription and Synthesis of Plasma Proteins1. The Journal Of Biochemistry 1995, 118: 921-931. PMID: 8749308, DOI: 10.1093/jb/118.5.921.Peer-Reviewed Original ResearchConceptsSubtractive cDNA libraryOcular ciliary bodyCDNA clonesCDNA libraryCell-restricted expressionPartial DNA sequencesCell-specific genesPotential candidate genesSubtractive libraryComplement component C4Significant homologyHomology searchProtein databaseDNA sequencesCiliary bodyCandidate genesTranscriptional expressionNorthern hybridizationSignificant genesGenesIntraocular pressureCiliary epithelial cellsPlasma proteinsProteinVascular endothelial cells
1992
Regulation of ICAM-1 expression on human non-pigmented ciliary body epithelial (NPE) and trabecular meshwork (HTM) cells
Aoki K, Kropp M, Bullington R, Gil D, Coca-Prados M, WoldeMussie E, Wheeler L. Regulation of ICAM-1 expression on human non-pigmented ciliary body epithelial (NPE) and trabecular meshwork (HTM) cells. Experimental Eye Research 1992, 55: 10. DOI: 10.1016/0014-4835(92)90248-q.Peer-Reviewed Original Research
1988
Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium
Helbig H, Korbmacher C, Stumpff F, Coca‐Prados M, Wiederholt M. Na+/H+ exchange regulates intracellular pH in a cell clone derived from bovine pigmented ciliary epithelium. Journal Of Cellular Physiology 1988, 137: 384-389. PMID: 2848042, DOI: 10.1002/jcp.1041370225.Peer-Reviewed Original ResearchConceptsPigmented ciliary epitheliumMM amilorideAcid loadCiliary epitheliumCell clonesPH-sensitive absorbanceBicarbonate-free mediumRegulation of intracellularCiliary bodyAmilorideExtracellular protonsBovine ciliary bodyEpitheliumConfluent monolayersIntracellularCellsCellular metabolismSlow acidificationAlkalinizationPlastic coverslips