2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2020
Claudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions
Liu F, Xu T, Peng S, Adelman RA, Rizzolo LJ. Claudins regulate gene and protein expression of the retinal pigment epithelium independent of their association with tight junctions. Experimental Eye Research 2020, 198: 108157. PMID: 32712183, DOI: 10.1016/j.exer.2020.108157.Peer-Reviewed Original Research
2019
Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function
Wang SB, Xu T, Peng S, Singh D, Ghiassi-Nejad M, Adelman RA, Rizzolo LJ. Disease-associated mutations of claudin-19 disrupt retinal neurogenesis and visual function. Communications Biology 2019, 2: 113. PMID: 30937396, PMCID: PMC6433901, DOI: 10.1038/s42003-019-0355-0.Peer-Reviewed Original ResearchConceptsRetinal pigment epitheliumClaudin-19Retinal neurogenesisP1 waveOuter nuclear layerRPE signature genesARPE19 cell lineOcular involvementKidney diseaseVisual functionFamilial hypomagnesaemiaNuclear layerBipolar cellsNewborn miceOcular diseasesPigment epitheliumRetinal isomeraseDiseaseMiceHuman induced pluripotent cellsRetinal differentiationSignature genesCell linesNeurogenesisInduced pluripotent cells
2009
Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions
Peng S, Adelman RA, Rizzolo LJ. Minimal Effects of VEGF and Anti-VEGF Drugs on the Permeability or Selectivity of RPE Tight Junctions. Investigative Ophthalmology & Visual Science 2009, 51: 3216-3225. PMID: 20042644, PMCID: PMC2891474, DOI: 10.1167/iovs.09-4162.Peer-Reviewed Original ResearchAngiogenesis InhibitorsAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedBevacizumabBlood-Retinal BarrierCapillary PermeabilityCells, CulturedClaudinsElectric ImpedanceEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectGene ExpressionHumansImmunoblottingPolyethylene GlycolsPotassiumRanibizumabRetinal Pigment EpitheliumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumTight JunctionsVascular Endothelial Growth Factor A
2002
Claudin 5 Is Transiently Expressed During the Development of the Retinal Pigment Epithelium
Kojima S, Rahner C, Peng S, Rizzolo L. Claudin 5 Is Transiently Expressed During the Development of the Retinal Pigment Epithelium. The Journal Of Membrane Biology 2002, 186: 81-88. PMID: 11944085, DOI: 10.1007/s00232-001-0137-7.Peer-Reviewed Original ResearchConceptsReverse transcriptase-polymerase chain reactionRetinal pigment epitheliumClaudin-5Chick retinal pigment epitheliumPigment epitheliumTranscriptase-polymerase chain reactionEmbryonic day 14Tight junctionsSemiquantitative RT-PCRExpression of claudinsEmbryonic day 10Northern blottingNeural retinaDay 14Semi-quantitative RT-PCRDay 10Claudin-3RPE developmentPeak levelsDay 7 embryosChoroid layerPrimary culturesChain reactionEpitheliumBlotting