Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry Complements Traditional Phosphoproteomic Analysis via Influencing Charge State Distribution of Phosphopeptides
Wang Y, Gao J, Xie W, Tang M, Chen X, Chen L, Chen H, Yang Z, Gao Q, Liu Y, Zhou H. Online Alkaline-pH Reversed-Phase Nanoelectrospray-Tandem Mass Spectrometry Complements Traditional Phosphoproteomic Analysis via Influencing Charge State Distribution of Phosphopeptides. Journal Of Proteome Research 2025, 24: 2443-2453. PMID: 40205994, DOI: 10.1021/acs.jproteome.4c01091.Peer-Reviewed Original ResearchConceptsNanoelectrospray tandem mass spectrometryPhosphopeptide enrichment techniquesNanoelectrospray ionizationTandem MSMass spectrometerC18 columnNormal adjacent tissuesMass spectrometryPairs of hepatocellular carcinomaPhosphorylated peptidesLiquid chromatography fractionationPhosphopeptidesHigh pHActual polarizationBiological functionsPhosphoproteomic analysisChromatography fractionsIonizationSpectrometryLow pHEnrichment techniquesHepatocellular carcinomaSpectrometerAdjacent tissuesGlobal phosphorylation
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