2022
Ancestral archaea expanded the genetic code with pyrrolysine
Guo LT, Amikura K, Jiang HK, Mukai T, Fu X, Wang YS, O’Donoghue P, Söll D, Tharp JM. Ancestral archaea expanded the genetic code with pyrrolysine. Journal Of Biological Chemistry 2022, 298: 102521. PMID: 36152750, PMCID: PMC9630628, DOI: 10.1016/j.jbc.2022.102521.Peer-Reviewed Original ResearchConceptsAminoacylation efficiencyGenetic code expansionDomains of lifePyrrolysyl-tRNA synthetaseTRNA-binding domainFull-length enzymeNoncanonical amino acidsAmino acid substratesMolecular phylogenyDiverse archaeaCoevolutionary historyTRNA sequencesGenetic codeCode expansionDiscriminator basesMethanogenic archaeaMethanosarcina mazeiPylRSSubstrate spectrumTRNAArchaeaMultiple organismsLiving cellsAcid substratesAmino acids
2017
The Role of mRNA Degradation in Dynamic Nitrogen Environments in Saccharomyces cerevisiae
Rodriguez‐Tirado C, Gresham D, Abdul‐Rahman F. The Role of mRNA Degradation in Dynamic Nitrogen Environments in Saccharomyces cerevisiae. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.596.4.Peer-Reviewed Original ResearchNitrogen catabolite repressionReprogramming of gene expressionNitrogen-poor conditionsMRNA degradationUntranslated regionGene expressionAmino acid permeaseNitrogen-rich conditionsSaccharomyces cerevisiae cellsNitrogen-poor environmentsKnock-outAmino acid substratesNitrogen-rich environmentCatabolite repressionSaccharomyces cerevisiaeIdentified mutantsGrowth defectRegulates localizationGAP1MutantsStem cell differentiationAccelerated degradationGrowth rateAcid substratesPlasma membrane
2009
Aminopeptidase Fingerprints, an Integrated Approach for Identification of Good Substrates and Optimal Inhibitors*
Drag M, Bogyo M, Ellman JA, Salvesen GS. Aminopeptidase Fingerprints, an Integrated Approach for Identification of Good Substrates and Optimal Inhibitors*. Journal Of Biological Chemistry 2009, 285: 3310-3318. PMID: 19948737, PMCID: PMC2823418, DOI: 10.1074/jbc.m109.060418.Peer-Reviewed Original ResearchConceptsSubstrate specificityAminopeptidase NS1 pocketN-terminal amino acidsAmino acid substratesCell surface proteaseTypes of proteasesProteolytic eventsCell survivalRat orthologAcid substratesSurface proteaseSpecific inhibitorAmino acidsSubstrate hydrolysisCell compartmentTarget substrateAminopeptidasesGood substrateOptimal inhibitorSequential cleavageProteaseBioactive peptidesEukaryotesOrthologs
2008
A Competitive Inhibitor Traps LeuT in an Open-to-Out Conformation
Singh SK, Piscitelli CL, Yamashita A, Gouaux E. A Competitive Inhibitor Traps LeuT in an Open-to-Out Conformation. Science 2008, 322: 1655-1661. PMID: 19074341, PMCID: PMC2832577, DOI: 10.1126/science.1166777.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid Transport SystemsAmino AcidsBacterial ProteinsBinding SitesBinding, CompetitiveBiological TransportCrystallizationCrystallography, X-RayHydrogen BondingHydrophobic and Hydrophilic InteractionsKineticsLeucineLigandsModels, BiologicalModels, MolecularProtein ConformationProtein Structure, TertiarySodiumSymportersTryptophanConceptsNeurotransmitter sodium symportersPrimary substrate siteAmino acid substratesSodium symportersSecondary transportersExtracellular gateSubstrate passageLeucine transporterArginine 30Substrate transportCellular membranesConformational changesAcid substratesConformational statesSubstrate siteFunctional studiesIon gradientsLeuTWeak binding sitesTransportersBinding sitesSmall moleculesCompetitive inhibitorConformationTrp complex
2002
tRNA‐dependent amino acid discrimination by yeast seryl‐tRNA synthetase
Gruic‐Sovulj I, Landeka I, Söll D, Weygand‐Durasevic I. tRNA‐dependent amino acid discrimination by yeast seryl‐tRNA synthetase. The FEBS Journal 2002, 269: 5271-5279. PMID: 12392560, DOI: 10.1046/j.1432-1033.2002.03241.x.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseYeast seryl-tRNA synthetaseCognate tRNA moleculesAmino acid discriminationAminoacyl-tRNA synthetasesAmino acid substratesSimilar amino acidsAmino acid serineGenetic codeEnzyme active siteTRNA moleculesActive siteYeast SerRSConformational changesAcid substratesAmino acidsSerineSynthetaseStoichiometric analysisDifferent affinitiesEnzymeAccurate translationTRNASerSynthetasesSaccharomyces
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply