Determining antigen specificity of a monoclonal antibody using genome-scale CRISPR-Cas9 knockout library
Zotova A, Zotov I, Filatov A, Mazurov D. Determining antigen specificity of a monoclonal antibody using genome-scale CRISPR-Cas9 knockout library. Journal Of Immunological Methods 2016, 439: 8-14. PMID: 27664857, DOI: 10.1016/j.jim.2016.09.006.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntibody SpecificityCell LineCell SeparationCRISPR-Cas SystemsEpitopesFlow CytometryGene EditingGene Knockout TechniquesGene LibraryHigh-Throughput Nucleotide SequencingHuman T-lymphotropic virus 1HumansHybridomasKangai-1 ProteinLeukocyte Common AntigensTransfectionConceptsShort-interfering RNAGuide-RNACRISPR-Cas9 knockout libraryGene-based methodsRaji B cell lineCRISPR-Cas9 knockoutDeep sequencing analysisGene-based techniquesGeCKO libraryProtein-based methodsKnockout libraryLibrary preparationValidation of antibodiesB cell linesPurified proteinPooled librariesCDNA expressionImmunoprecipitation assaysWhole cellsMonoclonal antibodiesCell sortingViral biofilmsCurrent proteinsAntibody validationAntigen-Specific
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply