2024
Lipocoacervate, a tunable vesicle for protein delivery
Yeh C, Wright N, Loh C, Chu N, Wang Y. Lipocoacervate, a tunable vesicle for protein delivery. Nano Research 2024, 17: 9135-9140. DOI: 10.1007/s12274-024-6889-6.Peer-Reviewed Original ResearchProtein delivery vehiclesDelivery of proteinsCells in vitroGrowth factorVesiclesProteinMembrane functionControlled delivery of growth factorsTreated cells in vitroProtein deliveryControlled DeliveryControlled delivery of proteinsDelivery of therapeuticsSalt concentrationDelivery of growth factorsMembraneVesicle sizeControlled delivery of therapeutics
2022
Assembly and architecture of the type III secretion sorting platform
Soto J, Galán J, Lara-Tejero M. Assembly and architecture of the type III secretion sorting platform. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2218010119. PMID: 36512499, PMCID: PMC9907115, DOI: 10.1073/pnas.2218010119.Peer-Reviewed Original ResearchConceptsType III secretion machinesType III secretion systemTarget eukaryotic cellsType III secretionSecretion of proteinsBacterial nanomachinesSecretion machineEukaryotic cellsExport pathwayImportant bacterial pathogensSecretion systemBacterial structureAntivirulence strategiesCoordinated mechanismFunctional complexityBacterial pathogensGenetic deletionStructure modelingProtein deliveryAssemblyRational developmentCross-linking strategyAssembly processProteinDeletion
2021
ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER
Weigel AV, Chang CL, Shtengel G, Xu CS, Hoffman DP, Freeman M, Iyer N, Aaron J, Khuon S, Bogovic J, Qiu W, Hess HF, Lippincott-Schwartz J. ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. Cell 2021, 184: 2412-2429.e16. PMID: 33852913, DOI: 10.1016/j.cell.2021.03.035.Peer-Reviewed Original ResearchConceptsTubular networkEarly secretory compartmentsAccurate traffickingProtein exportDiverse proteinsProtein localizationSecretory pathwayMammalian cellsSecretory compartmentsCargo entryGolgi apparatusLipid bilayersIllumination microscopyBeam scanning electron microscopyIon beam scanning electron microscopyProtein deliveryCOPIIERDynamic 3D viewsTraffickingMicrotubulesProteinVesiclesPathwayCompartments
2019
Recoding the metagenome: microbiome engineering in situ
Whitfill T, Oh J. Recoding the metagenome: microbiome engineering in situ. Current Opinion In Microbiology 2019, 50: 28-34. PMID: 31622928, DOI: 10.1016/j.mib.2019.09.005.Peer-Reviewed Original Research
2017
Assessment of Protein Turnover in Health and Disease
Wernerman J, Morris C, Paddon‐Jones D, Sarav M. Assessment of Protein Turnover in Health and Disease. Nutrition In Clinical Practice 2017, 32: 15s-20s. PMID: 28388375, DOI: 10.1177/0884533617694611.Peer-Reviewed Original ResearchDevelopment of an inducible platform for intercellular protein delivery
Siller R, Dufour E, Lycke M, Wilmut I, Jung YW, Park IH, Sullivan GJ. Development of an inducible platform for intercellular protein delivery. International Journal Of Pharmaceutics 2017, 522: 1-10. PMID: 28254654, DOI: 10.1016/j.ijpharm.2017.02.067.Peer-Reviewed Original ResearchConceptsNovel platformBiomolecules of interestCell penetrating peptideGene of interestHuman chorionic gonadotropin beta subunitProtein deliveryStem cell biologyChorionic gonadotropin beta subunitPenetrating peptidePotential applicationsProtein transduction domainProtein of interestGreen fluorescent proteinFusion proteinGonadotropin beta subunitEfficient uptakeN-terminal signal peptideCell culture mediumPlatformFluorescent proteinTransduction domainLack of specificityBeta subunitActive proteinFixation process
2015
The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135
Stoops EH, Hull M, Olesen C, Mistry K, Harder JL, Rivera-Molina F, Toomre D, Caplan MJ. The periciliary ring in polarized epithelial cells is a hot spot for delivery of the apical protein gp135. Journal Of Cell Biology 2015, 211: 287-294. PMID: 26504168, PMCID: PMC4621837, DOI: 10.1083/jcb.201502045.Peer-Reviewed Original ResearchConceptsPrimary ciliaSurface proteinsTrans-Golgi networkPolarized epithelial cellsApical surface proteinsSNAP-tag systemBasolateral plasma membraneCell surface proteinsEpithelial cellsApical proteinsPericiliary regionGolgi networkPolarized traffickingCarrier vesiclesProtein deliveryPlasma membraneApical membraneProteinGp135Basolateral membraneCiliaMembraneHot spotsCellsTrafficking
2014
Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells
Galán JE, Lara-Tejero M, Marlovits TC, Wagner S. Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells. Annual Review Of Microbiology 2014, 68: 1-24. PMID: 25002086, PMCID: PMC4388319, DOI: 10.1146/annurev-micro-092412-155725.Peer-Reviewed Original ResearchConceptsEukaryotic cellsEffector proteinsBacterial envelopeType III secretion systemTarget eukaryotic cellsComplex nanomachinesNovel antimicrobial strategiesPathogen's benefitCellular functionsSecretion systemNeedle complexBacterial pathogenesisCytoplasmic componentsSpecialized nanomachinesSpecific substratesProteinPlantsCentral roleProtein deliveryAntimicrobial strategiesBacteriaNanomachinesTarget cellsCentral componentCells
2009
Engineered Molecular Delivery for Control and Enhancement of Transplanted Endothelial Cell Fate in Tissue Engineering
Jay S, Shepherd B, Andrejecsk J, Kyriakides T, Pober J, Saltzman W. Engineered Molecular Delivery for Control and Enhancement of Transplanted Endothelial Cell Fate in Tissue Engineering. 2009, 1: 1-2. DOI: 10.1109/nebc.2009.4967788.Peer-Reviewed Original ResearchTissue engineeringAdvanced tissue engineeringMultifunctional hydrogelsTissue constructsFunctional microvascular networksTransplantation of ECsEndothelial cell fateBiomaterial strategiesAlginate microparticlesVascular endothelial growth factorEndothelial cellsEngineeringHydrogelsCell fateGrowth factorSustained releaseProtein 1Mural cellsMolecular deliveryMonocyte chemoattractant protein-1Cell populationsProtein therapeuticsProtein matrixAngiogenic growth factorsProtein delivery
1999
Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán J, Collmer A. Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells. Science 1999, 284: 1322-1328. PMID: 10334981, DOI: 10.1126/science.284.5418.1322.Peer-Reviewed Original ResearchConceptsSecretion systemComplex protein secretion systemsHost cellsType III secretion systemBacterial effector proteinsProtein secretion systemAnimal pathogenic bacteriaHost cellular functionsGram-negative pathogenic bacteriaBacterial devicesPathogenic bacteriaEffector proteinsCellular functionsFlagellar apparatusBacterial speciesEffector moleculesProtein deliveryBacteriaCellsNovel preventionBiologySpeciesPlantsProteinTherapeutic approaches
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