2024
Increasing the Level of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the <i>CXCR4</i> Locus in the CEM/R5 T Cell Line
Golubev D, Komkov D, Shepelev M, Mazurov D, Kruglova N. Increasing the Level of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line. Молекулярная Биология 2024, 58 DOI: 10.31857/s0026898424040044.Peer-Reviewed Original ResearchNuclear localization signalNonhomologous end-joining pathwayEnd-joining pathwayKnock-inKnock-in modelDNA repairDNA-dependent protein kinase inhibitorT cell linesBlock DNA repairGenome editing technologyPeptide fusion inhibitorsTranscription factor NF-kBLocalization signalCXCR4 locusDonor plasmidCas9 nucleaseCas9 proteinDNA modificationsPrimary human cellsProtein kinase inhibitorsHIV-1Transporter sequencesInhibit DNA repairPlasmid transportEffective gene therapy approach[Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line].
Golubev D, Komkov D, Shepelev M, Mazurov D, Kruglova N. [Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line]. Молекулярная Биология 2024, 58: 575-589. PMID: 39709562, DOI: 10.31857/s0026898424040044, edn: incwav.Peer-Reviewed Original ResearchConceptsNuclear localization signalNonhomologous End JoiningDNA nuclear targeting sequencesKnock-inDNA repairNonhomologous end-joining pathwayNuclear targeting sequenceCXCR4 locusDNA-dependent protein kinase inhibitorBlock DNA repairKnock-in efficiencyEffective gene therapy approachGenome editing technologyTranscription factor NF-kBLocalization signalTreat HIV infectionGene therapy approachesTarget sequenceDonor plasmidCas9 nucleaseCas9 proteinEnd joiningDNA modificationsPrimary human cellsProtein kinase inhibitors
2023
Quantification of cell energetics in human subcutaneous adipose progenitor cells after target gene knockdown
Li L, Gunewardena A, Nyima T, Feldman B. Quantification of cell energetics in human subcutaneous adipose progenitor cells after target gene knockdown. STAR Protocols 2023, 4: 102607. PMID: 37742183, PMCID: PMC10751552, DOI: 10.1016/j.xpro.2023.102607.Peer-Reviewed Original ResearchHedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells
Jiang B, Wang S, Song G, Jiang Q, Fan M, Fang C, Li X, Soh C, Manes T, Cheru N, Qin L, Ren P, Jortner B, Wang Q, Quaranta E, Yoo P, Geirsson A, Davis R, Tellides G, Pober J, Jane-Wit D. Hedgehog-induced ZFYVE21 promotes chronic vascular inflammation by activating NLRP3 inflammasomes in T cells. Science Signaling 2023, 16: eabo3406. PMID: 36943921, PMCID: PMC10061549, DOI: 10.1126/scisignal.abo3406.Peer-Reviewed Original ResearchConceptsIschemia-reperfusion injuryChronic vascular inflammationT cellsNLRP3 inflammasomeVascular inflammationChronic inflammationEndothelial cellsIFN-γ responsesControl T cellsNLRP3 inflammasome activityT memory cellsAllograft vasculopathyVascular sequelaeHuman endothelial cellsCoronary arteryEffector responsesCell-autonomous roleInflammasome activityMouse modelInflammationPatient samplesVigorous recruitmentInflammasomePrimary human cellsImmune signaling
2020
Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome
Shchukina I, Bagaitkar J, Shpynov O, Loginicheva E, Porter S, Mogilenko DA, Wolin E, Collins P, Demidov G, Artomov M, Zaitsev K, Sidorov S, Camell C, Bambouskova M, Arthur L, Swain A, Panteleeva A, Dievskii A, Kurbatsky E, Tsurinov P, Chernyatchik R, Dixit VD, Jovanovic M, Stewart SA, Daly MJ, Dmitriev S, Oltz EM, Artyomov MN. Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome. Nature Aging 2020, 1: 124-141. PMID: 34796338, PMCID: PMC8597198, DOI: 10.1038/s43587-020-00002-6.Peer-Reviewed Original ResearchConceptsDNA methylomeAge-associated transcriptional changesPromoters of lowlyDNA methylation profilingHuman classical monocytesCorresponding genesPrimary human cellsTranscriptional changesEpigenetic profilingDNA methylationCpG islandsProteomic alterationsSpecific signaturesHypermethylation eventsMethylation profilingHuman cellsMolecular programmingMethylomeGenesMetabolomics dataProfilingComprehensive characterizationHuman monocytesH3K4me1H3K27me3
2017
An inflammatory bowel disease–risk variant in INAVA decreases pattern recognition receptor–induced outcomes
Yan J, Hedl M, Abraham C. An inflammatory bowel disease–risk variant in INAVA decreases pattern recognition receptor–induced outcomes. Journal Of Clinical Investigation 2017, 127: 2192-2205. PMID: 28436939, PMCID: PMC5451247, DOI: 10.1172/jci86282.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusCarrier ProteinsCase-Control StudiesCytokinesEnterococcus faecalisGene ExpressionGenetic Association StudiesGenetic Predisposition to DiseaseHEK293 CellsHeterozygoteHumansInflammatory Bowel DiseasesMacrophagesMAP Kinase Signaling SystemMyeloid CellsPolymorphism, Single NucleotidePrimary Cell CultureReceptors, Pattern RecognitionRisk FactorsStaphylococcus aureusConceptsInflammatory bowel diseasePattern recognition receptor signalingDisease risk variantsIntestinal immune homeostasisActivation of MAPKIBD risk lociINAVAPrimary human cellsBacterial clearanceIntestinal myeloid cellsRisk lociAutophagy pathwayProper regulationIntronic regionsHuman cellsImmune homeostasisReceptor signalingDownstream signalsPRR stimulationReactive oxygenIntestinal microbesNF-κB activationGenesNF-κB pathwayMAPK
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbers
2008
NFBD1/MDC1, 53BP1 and BRCA1 have both redundant and unique roles in the ATM pathway
Wilson KA, Stern DF. NFBD1/MDC1, 53BP1 and BRCA1 have both redundant and unique roles in the ATM pathway. Cell Cycle 2008, 7: 3584-3594. PMID: 19001859, PMCID: PMC2763172, DOI: 10.4161/cc.7.22.7102.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAtaxia Telangiectasia Mutated ProteinsBRCA1 ProteinCell Cycle ProteinsCell LineCheckpoint Kinase 2DNA-Binding ProteinsFibroblastsHumansIntracellular Signaling Peptides and ProteinsNuclear ProteinsPhosphorylationProtein Serine-Threonine KinasesRadiation, IonizingRNA, Small InterferingTrans-ActivatorsTumor Suppressor p53-Binding Protein 1Tumor Suppressor ProteinsConceptsNFBD1/MDC1DNA damage checkpoint proteinsRadiation-induced phosphorylationATM-Chk2 pathwayNormal genetic backgroundBRCT domainCheckpoint responseRedundant functionsPrimary human cellsRedundant rolesATM pathwayNFBD1Checkpoint proteinsMouse cellsHuman cellsGenetic backgroundMDC1Cancer cellsLocalization eventsPhosphorylationBRCA1Unique rolePathwayCellsHuman foreskin
2002
Role for the Related Poly(ADP-Ribose) Polymerases Tankyrase 1 and 2 at Human Telomeres
Cook BD, Dynek JN, Chang W, Shostak G, Smith S. Role for the Related Poly(ADP-Ribose) Polymerases Tankyrase 1 and 2 at Human Telomeres. Molecular And Cellular Biology 2002, 22: 332-342. PMID: 11739745, PMCID: PMC134233, DOI: 10.1128/mcb.22.1.332-342.2002.Peer-Reviewed Original ResearchConceptsTankyrase 2Tankyrase 1Telomeric complexTelomeric DNA-binding proteinAccess of telomerasePolymerase tankyrase 1DNA-binding proteinsSpecialized reverse transcriptaseEndogenous TRF1Telomere maintenanceTelomeric functionPrimary human cellsRelated homologsPARP domainTelomeric DNAHuman telomeresTRF1TelomeresHuman cellsADP-ribosylationPARP assaysRecent identificationTelomeraseReverse transcriptaseOverexpression
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