2024
Enhanced eMAGE applied to identify genetic factors of nuclear hormone receptor dysfunction via combinatorial gene editing
Ciaccia P, Liang Z, Schweitzer A, Metzner E, Isaacs F. Enhanced eMAGE applied to identify genetic factors of nuclear hormone receptor dysfunction via combinatorial gene editing. Nature Communications 2024, 15: 5218. PMID: 38890276, PMCID: PMC11189492, DOI: 10.1038/s41467-024-49365-z.Peer-Reviewed Original ResearchConceptsGenome modificationEngineered nucleasesMultiplex genome engineeringEfficient multiplex editingLigand-binding domain of human estrogen receptor alphaMethods of genome editingCancer-associated mutationsHomology-directed repairMismatch repair systemLigand-binding domainSaccharomyces cerevisiaeYeast modelSynthetic genomesGenome engineeringPolygenic basisComplex phenotypesBackground mutationsGenomeGenome editingMultiplex editingEditing frequencyHuman estrogen receptor alphaDNA breaksEstrogen receptor alphaMMR inactivationThe RRE-REV module has no effect on the packaging efficiency of cas9 and Gag proteins into nanomedic virus-like particles
Kruglova N, Komkov D, Mazurov D, Shepelev M. The RRE-REV module has no effect on the packaging efficiency of cas9 and Gag proteins into nanomedic virus-like particles. Доклады Российской Академии Наук Науки О Жизни 2024, 515: 64-70. DOI: 10.31857/s2686738924020121.Peer-Reviewed Original ResearchRev expression plasmidVirus-like particlesEmpty control plasmidGene therapy of human diseasesGag proteinTherapy of human diseasesGene therapyViral Gag proteinTarget cellsControl plasmidProtein levelsCas9 nucleaseGenome editingGagEfficiency of genome editingMethods of genome editingExpression of Cas9Plasmid constructsCotransfectionHuman diseasesPlasmidCell lysatesNuclear export
2023
The RRE–Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Kruglova N, Komkov D, Mazurov D, Shepelev M. The RRE–Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles. Doklady Biological Sciences 2023, 513: s45-s50. PMID: 38472686, DOI: 10.1134/s0012496623700886.Peer-Reviewed Original ResearchConceptsRev expression plasmidVirus-like particlesRev response elementEmpty control plasmidGene therapy of human diseasesGag proteinTherapy of human diseasesCas9 nucleaseGene therapyGenome editingGag expressionViral Gag proteinHIV Rev response elementTarget cellsEfficiency of genome editingMethods of genome editingControl plasmidProtein levelsNuclear exportNO effectPlasmid modificationGagResponse elementAccessory proteinsPlasmid constructs
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