Native Top–Down Analysis of Membrane Protein Complexes Directly From In Vitro and Native Membranes
Jung W, Panda A, Lee J, Ghosh S, Shaw J, Gupta K. Native Top–Down Analysis of Membrane Protein Complexes Directly From In Vitro and Native Membranes. Molecular & Cellular Proteomics 2025, 24: 100993. PMID: 40378922, PMCID: PMC12305242, DOI: 10.1016/j.mcpro.2025.100993.Peer-Reviewed Original ResearchNative mass spectrometryProtein complexesMembrane proteinsBAM complexTop-down MS/MSAnalysis of membrane protein complexesMembrane-associated protein complexesTarget membrane proteinsNative membranesIntegral membrane proteinsTop-down identificationMembrane protein complexesCell-derived membrane vesiclesMass spectrometryMembrane vesiclesOrganization of proteinsDestabilize lipid bilayersAntibiotic targetsProtein-lipid complexesHeteromeric natureSynthetic liposomesCellular membranesCo-factorNative membrane vesiclesTotal membranes
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