2023
Increasing sensitivity of antibody-antigen interactions using photo-cross-linking
de la Peña A, Sewall L, de Paiva Froes Rocha R, Jackson A, Pratap P, Bangaru S, Cottrell C, Mohanty S, Shaw A, Ward A. Increasing sensitivity of antibody-antigen interactions using photo-cross-linking. Cell Reports Methods 2023, 3: 100509. PMID: 37426749, PMCID: PMC10326447, DOI: 10.1016/j.crmeth.2023.100509.Peer-Reviewed Original ResearchConceptsPolyclonal immune responseImmune responseTime pointsRational vaccine designLater time pointsEarly time pointsVaccine immunogensAnimal modelsAntibody detectionVaccine designIntermediate antibodiesEarly timepointsLater timepointsAntibodiesViral glycoproteinsTimepointsDifferent viral glycoproteinsSensitivity of detectionAntibody-antigen interactionsResponsePatientsVaccination
2009
Redesign of a protein–peptide interaction: Characterization and applications
Jackrel ME, Valverde R, Regan L. Redesign of a protein–peptide interaction: Characterization and applications. Protein Science 2009, 18: 762-774. PMID: 19309728, PMCID: PMC2762588, DOI: 10.1002/pro.75.Peer-Reviewed Original ResearchConceptsProtein-peptide interactionsProtein-peptide pairsPurification applicationsRecognition pairsCorresponding peptide ligandsRepeat protein scaffoldsMolecular recognitionAntibody-antigen interactionsFacile replacementChemical considerationsProtein scaffoldsPeptide ligandsTypical antibody-antigen interactionsHarsh conditionsProtein designModerate affinityScaffoldsPractical applicationsAffinity purificationAffinityLigandsPropertiesInteractionSuccessful applicationDissociation
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