2022
Degradation of gene silencer is essential for expression of foreign genes and bacterial colonization of the mammalian gut
Choi J, Schmukler M, Groisman EA. Degradation of gene silencer is essential for expression of foreign genes and bacterial colonization of the mammalian gut. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2210239119. PMID: 36161931, PMCID: PMC9546599, DOI: 10.1073/pnas.2210239119.Peer-Reviewed Original ResearchConceptsH-NSForeign genesHeat-stable nucleoid-structuring (H-NS) proteinWild-type H-NSNucleoid-associated proteinsH-NS proteinsNucleoid structuring proteinHorizontal gene transferBacterial evolutionLon proteaseSilences expressionCorresponding genesForeign DNAMammalian gutMammalian hostsStructuring proteinGene silencersGenesMurine gutEnteric bacteriaGene transferBiofilm formationProteinGeneral mechanismColonization
2020
Salmonella expresses foreign genes during infection by degrading their silencer
Choi J, Groisman EA. Salmonella expresses foreign genes during infection by degrading their silencer. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 8074-8082. PMID: 32209674, PMCID: PMC7149492, DOI: 10.1073/pnas.1912808117.Peer-Reviewed Original ResearchConceptsForeign genesH-NSForeign DNAHeat-stable nucleoid-structuring (H-NS) proteinH-NS bindingNucleoid structuring proteinGram-negative bacterial speciesFacultative intracellular pathogenPhoP proteinLon proteaseProtease LonRegulatory sequencesVirulence regulatorGene silencingIntramacrophage survivalStructuring proteinBacterial speciesGenesInside macrophagesIntracellular pathogensSerovar TyphimuriumUncovered mechanismProteinVirulence genesDNA
2008
Overcoming H-NS-mediated Transcriptional Silencing of Horizontally Acquired Genes by the PhoP and SlyA Proteins in Salmonella enterica *
Perez JC, Latifi T, Groisman EA. Overcoming H-NS-mediated Transcriptional Silencing of Horizontally Acquired Genes by the PhoP and SlyA Proteins in Salmonella enterica *. Journal Of Biological Chemistry 2008, 283: 10773-10783. PMID: 18270203, PMCID: PMC2447644, DOI: 10.1074/jbc.m709843200.Peer-Reviewed Original ResearchConceptsHistone-like nucleoid structuring proteinPagC geneSlyA proteinRNA polymeraseRNA polymerase recruitmentH-NS repressionNucleoid structuring proteinHorizontal gene transferDifferent regulatory proteinsSalmonella entericaPolymerase recruitmentTranscriptional silencingNew traitsForeign DNAGene transcriptionRegulatory proteinsUgtLRespective promotersTranscriptionPhoPRecipient organismGenesPagC promoterGene transferProtein
2001
Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar M, Keller B, Makalou N, Sutton R. Systematic Determination of the Packaging Limit of Lentiviral Vectors. Human Gene Therapy 2001, 12: 1893-1905. PMID: 11589831, DOI: 10.1089/104303401753153947.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsBlotting, SouthernCapsidCell LineDNAEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesGenetic VectorsHeLa CellsHIV-1HumansLentivirusMembrane GlycoproteinsModels, GeneticPlasmidsReverse Transcriptase Polymerase Chain ReactionRNA, ViralTransduction, GeneticTransfectionViral Envelope ProteinsConceptsPackaging limitLentiviral vectorsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1Oncoretroviral vectorsTherapeutic deliveryVesicular stomatitis virus G proteinMeasurable titersLentiviral particlesLow titersViral titersType 1Great potentialForeign DNATitersProviral RNAVirus G proteinViral encapsidationHIVG proteinsLarge vectorsChromosomal DNA fragmentLimited utilityCells
1999
cis Requirements for the Efficient Production of Recombinant DNA Vectors Based on Autonomous Parvoviruses
Kestler J, Neeb B, Struyf S, Van Damme J, Cotmore S, D'Abramo A, Tattersall P, Rommelaere J, Dinsart C, Cornelis J. cis Requirements for the Efficient Production of Recombinant DNA Vectors Based on Autonomous Parvoviruses. Human Gene Therapy 1999, 10: 1619-1632. PMID: 10428207, DOI: 10.1089/10430349950017626.Peer-Reviewed Original ResearchConceptsViral vectorsViral vector productionRecombinant viral vectorsRecombinant DNA vectorsVector productionRecombinant parvovirusesDNA vectorsRecombinant particlesVirus vectorsOverall sizeRecombinant vectorSmall transgenesEfficient productionHelper plasmidForeign DNAEfficient packagingPlasmid DNAParvovirus MVMpRecombinant clonesAutonomous parvovirusesViral terminiInfectious unitsVP sequencesDNA lengthVector
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