2020
The pseudoGTPase group of pseudoenzymes
Stiegler AL, Boggon TJ. The pseudoGTPase group of pseudoenzymes. The FEBS Journal 2020, 287: 4232-4245. PMID: 32893973, PMCID: PMC7544640, DOI: 10.1111/febs.15554.Peer-Reviewed Original ResearchConceptsSignal transductionAmino acid divergenceEnzymatic activityLeucine-rich repeat kinase 2Amino acid differencesP190RhoGAP proteinsRepeat kinase 2Membrane traffickingCellular rolesEnzyme foldImportant mechanistic componentSmall GTPasesTranscriptional controlCellular functionsGTPasesKinase 2PseudoenzymesAcid differencesEC numbersCell migrationFunctional roleMitochondrial activityProteinCargo transportMechanistic components
2016
A Molecular Determinant of Subtype-Specific Desensitization in Ionotropic Glutamate Receptors
Alsaloum M, Kazi R, Gan Q, Amin J, Wollmuth LP. A Molecular Determinant of Subtype-Specific Desensitization in Ionotropic Glutamate Receptors. Journal Of Neuroscience 2016, 36: 2617-2622. PMID: 26937003, PMCID: PMC4879209, DOI: 10.1523/jneurosci.2667-15.2016.Peer-Reviewed Original ResearchConceptsIon channelsIonotropic glutamate receptorsHydrophobic boxDiverse animal phylaExtensive sequence alignmentDiverse animal speciesGlutamate-gated ion channelsSingle amino acid differenceExchange mutationAmino acid differencesFast excitatory synaptic transmissionAvailable structural informationAnimal phylaTransmembrane helicesSequence alignmentGlutamate receptorsAcid differencesMolecular determinantsGluN2 subunitsAnimal speciesExtracellular interfaceMotifSynaptic functionReceptor functionNervous system
2012
An Allele of an Ancestral Transcription Factor Dependent on a Horizontally Acquired Gene Product
Chen HD, Jewett MW, Groisman EA. An Allele of an Ancestral Transcription Factor Dependent on a Horizontally Acquired Gene Product. PLOS Genetics 2012, 8: e1003060. PMID: 23300460, PMCID: PMC3531487, DOI: 10.1371/journal.pgen.1003060.Peer-Reviewed Original ResearchConceptsPolymyxin B resistanceGene productsTranscription of PmrAGene regulatory circuitsB resistanceSingle amino acid differenceAmino acid differencesPmrA proteinAncestral genomesAncestral genePmrD proteinRegulatory circuitsB alleleHorizontal transferGene expressionPhenotypic differencesAcid differencesGenesBiochemical propertiesBiofilm formationPmrAAllelesSalmonella entericaS. entericaProtein
2007
Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †
Weerachayaphorn J, Pajor AM. Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †. Biochemistry 2007, 47: 1087-1093. PMID: 18161988, PMCID: PMC2570185, DOI: 10.1021/bi701417h.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionAnimalsChlorocebus aethiopsCitric AcidCOS CellsDicarboxylic Acid TransportersGene ExpressionHumansKineticsMolecular Sequence DataMutationOrganic Anion Transporters, Sodium-DependentRabbitsRecombinant ProteinsSequence Homology, Amino AcidSodiumSubstrate SpecificitySuccinic AcidSymportersThreonineConceptsTransmembrane helix 7Functional differencesApparent affinityApparent sodium affinitySubstrate Binding SiteAmino acid differencesCitric acid cycleCitrate affinityHelix 7Substrate specificityPosition -509Rabbit sequenceIndividual residuesAcid cycleAcid differencesSimilar apparent affinitiesLoop regionIntermediate KmAmino acidsBinding sitesMutantsNaDC1SerineThreonineExhibit differences
1999
Targeted deletion of the ATP binding domain of left-right dynein confirms its role in specifying development of left-right asymmetries
Supp D, Brueckner M, Kuehn M, Witte D, Lowe L, McGrath J, Corrales J, Potter S. Targeted deletion of the ATP binding domain of left-right dynein confirms its role in specifying development of left-right asymmetries. Development 1999, 126: 5495-5504. PMID: 10556073, PMCID: PMC1797880, DOI: 10.1242/dev.126.23.5495.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceAnimalsAxonemal DyneinsBinding SitesBody PatterningCatalytic DomainCiliaCloning, MolecularDyneinsFunctional LateralityGene Expression Regulation, DevelopmentalHeadMaleMiceMice, Inbred StrainsMolecular Sequence DataMutationNervous SystemSequence AnalysisSequence DeletionConceptsLeft-right dyneinLeft-right developmentLeft-right asymmetryEmbryonic day 8.0Microtubule-based motor proteinsAsymmetric expression patternLevel of sequenceComplete coding sequenceEmbryonic day 7.5Single amino acid differenceLeft-right specificationAmino acid differencesLeft-right axisLgl mutantsATP bindingConserved positionDay 8.0Inversus viscerum (iv) mouseCoding sequenceMotor proteinsDorsoventral axesExpression patternsGerm layersAcid differencesGenesMolecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1
Mortensen O, Kristensen A, Rudnick G, Wiborg O. Molecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1. Brain Research 1999, 71: 120-126. PMID: 10407194, DOI: 10.1016/s0169-328x(99)00178-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarrier ProteinsCattleCitalopramCloning, MolecularDesipramineFemaleFluoxetineHeLa CellsHumansImipramineKineticsMembrane GlycoproteinsMembrane Transport ProteinsMolecular Sequence DataNerve Tissue ProteinsN-Methyl-3,4-methylenedioxyamphetamineOrgan SpecificityParoxetinePhylogenyPregnancyRatsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSequence AlignmentSequence Homology, Amino AcidSerotoninSerotonin Plasma Membrane Transport ProteinsTransfectionConceptsSerotonin transporterHuman serotonin transporterExpression of SERTAdrenal glandBrain stemParathyroid glandsPharmacological profileBone marrowThyroid glandSmall intestinePharmacological targetsRT-PCR amplificationDecreased sensitivityExtracellular fluidGlandAmino acid differencesBiogenic aminesNeurotransmitter transportersDependent neurotransmitter transportersImportant antidepressantsAcid differencesDifferent tissuesAntidepressantsParoxetineDesipramine
1992
Truncation variants of peptides isolated from MHC class II molecules suggest sequence motifs
Rudensky A, Preston-Hurlburt, P, Al-Ramadi B, Rothbard J, Janeway C. Truncation variants of peptides isolated from MHC class II molecules suggest sequence motifs. Nature 1992, 359: 429-431. PMID: 1328884, DOI: 10.1038/359429a0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigen-Antibody ReactionsBacterial ProteinsBinding Sites, AntibodyCell LineChromatography, High Pressure LiquidHistocompatibility Antigens Class IIImmunoglobulin GImmunoglobulin Heavy ChainsImmunoglobulin Variable RegionMiceMice, Inbred C57BLMolecular Sequence DataPeptide FragmentsReceptors, TransferrinRepressor ProteinsSequence AlignmentSequence Homology, Amino AcidT-LymphocytesViral Envelope ProteinsConceptsMHC class II moleculesClass II moleculesMHC class IMajor histocompatibility complexCD4 T cell recognitionClass IForeign protein antigensMHC class IIT cell recognitionT cellsMHC moleculesClass IIProtein antigensHistocompatibility complexAntigenic peptidesOuter aspectPeptide-binding cleftAmino acid differencesAnchor residuesAllelic variantsSingle peptide sequenceDifferent allelic formsPeptidesTruncation variantsAllelic forms
1986
crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli
Cossart P, Groisman EA, Serre MC, Casadaban MJ, Gicquel-Sanzey B. crp genes of Shigella flexneri, Salmonella typhimurium, and Escherichia coli. Journal Of Bacteriology 1986, 167: 639-646. PMID: 3525518, PMCID: PMC212937, DOI: 10.1128/jb.167.2.639-646.1986.Peer-Reviewed Original ResearchConceptsE. coli crp geneNucleotide sequenceCatabolite gene activator proteinCRP geneGene activator proteinCrp gene productCorresponding amino acid sequencesE. coli CRPComplete nucleotide sequenceAmino acid divergenceEscherichia coli KAmino acid sequenceBase pair changesAmino acid differencesSalmonella typhimurium LT2S. typhimuriumRegulatory genesCodon usageActivator proteinRegulatory regionsGene productsAcid sequenceColi KPair changesAcid differences
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