2011
Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation
Magaldi TG, Almstead LL, Bellone S, Prevatt EG, Santin AD, DiMaio D. Primary human cervical carcinoma cells require human papillomavirus E6 and E7 expression for ongoing proliferation. Virology 2011, 422: 114-124. PMID: 22056390, PMCID: PMC3229657, DOI: 10.1016/j.virol.2011.10.012.Peer-Reviewed Original ResearchConceptsCervical carcinoma cellsCervical cancer cellsHuman papillomavirus E6Human cervical carcinoma cellsCarcinoma cellsPrimary cervical cancer cellsCancer cellsPapillomavirus E6Cervical carcinoma cell linesE2 proteinHuman cervical cancer cellsCarcinoma cell linesE7 expressionE7 oncogenesLow passage numberSerum-free conditionsCell surface receptorsSV40 infectionTumor suppressor pathwayCell linesPrimary cellsViral vectorsE6Suppressor pathwayPassage numberExpression of Tissue factor in Adenocarcinoma and Squamous Cell Carcinoma of the Uterine Cervix: Implications for immunotherapy with hI-con1, a factor VII-IgGFcchimeric protein targeting tissue factor
Cocco E, Varughese J, Buza N, Bellone S, Glasgow M, Bellone M, Todeschini P, Carrara L, Silasi DA, Azodi M, Schwartz PE, Rutherford TJ, Pecorelli S, Lockwood CJ, Santin AD. Expression of Tissue factor in Adenocarcinoma and Squamous Cell Carcinoma of the Uterine Cervix: Implications for immunotherapy with hI-con1, a factor VII-IgGFcchimeric protein targeting tissue factor. BMC Cancer 2011, 11: 263. PMID: 21693061, PMCID: PMC3141777, DOI: 10.1186/1471-2407-11-263.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinomaCarcinoma, Squamous CellCell Line, TumorComplement System ProteinsCytotoxicity Tests, ImmunologicDrug Screening Assays, AntitumorFemaleHuman papillomavirus 16Human papillomavirus 18HumansImmunoconjugatesImmunoglobulin GImmunotherapyInterleukin-2KeratinocytesMolecular Targeted TherapyNeoplasm ProteinsNeovascularization, PathologicPapillomavirus InfectionsRNA, MessengerRNA, NeoplasmThromboplastinUterine Cervical NeoplasmsConceptsCervical cancer cell linesPrimary cervical cancer cell linesCervical carcinoma cell linesCancer cell linesCervical cancerCarcinoma cell linesFactor VII/VIIaTissue factorUterine cervixCell linesImportant worldwide health problemTargeting tissue factorStandard treatment modalitySquamous cell carcinomaExpression of TFWorldwide health problemNovel therapeutic agentsNormal cervical keratinocytesAdenocarcinoma histologyBackgroundCervical cancerCancer refractoryRecurrent diseaseCell carcinomaTreatment modalitiesNovel therapies
2010
Primary Cervical Carcinoma Cell Lines Overexpress Epithelial Cell Adhesion Molecule (EpCAM) and Are Highly Sensitive to Immunotherapy With MT201, a Fully Human Monoclonal Anti-EpCAM Antibody
Richter CE, Cocco E, Bellone S, Bellone M, Casagrande F, Todeschini P, Rüttinger D, Silasi DA, Azodi M, Schwartz PE, Rutherford TJ, Pecorelli S, Santin AD. Primary Cervical Carcinoma Cell Lines Overexpress Epithelial Cell Adhesion Molecule (EpCAM) and Are Highly Sensitive to Immunotherapy With MT201, a Fully Human Monoclonal Anti-EpCAM Antibody. International Journal Of Gynecological Cancer 2010, 20: 1440-1447. PMID: 21370592, PMCID: PMC3701951, DOI: 10.1111/igc.0b013e3181fb18a1.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedAntigens, NeoplasmCarcinomaCell Adhesion MoleculesCell Culture TechniquesCell Line, TumorEpithelial Cell Adhesion MoleculeFemaleFlow CytometryGene Expression ProfilingGene Expression Regulation, NeoplasticHumansImmunotherapyMiddle AgedTreatment OutcomeUterine Cervical NeoplasmsYoung AdultConceptsCervical carcinoma cell linesEpithelial cell adhesion moleculeComplement-dependent cytotoxicityCervical cancer cell linesInterleukin-2Real-time polymerase chain reactionCarcinoma cell linesCell adhesion moleculeCancer cell linesAggressive tumorsPolymerase chain reactionAdhesion moleculesPrimary cervical cancer cell linesCell linesRelease assaysFlow cytometryHighest messenger RNA expressionStandard salvage therapyCell adhesion molecule expressionEffective treatment optionAdhesion molecule expressionChain reactionHuman monoclonal antibodyMessenger RNA expressionEpithelial cell adhesion molecule (EpCAM) expression
2009
Notch1 regulates the functional contribution of RhoC to cervical carcinoma progression
Srivastava S, Ramdass B, Nagarajan S, Rehman M, Mukherjee G, Krishna S. Notch1 regulates the functional contribution of RhoC to cervical carcinoma progression. British Journal Of Cancer 2009, 102: 196-205. PMID: 19953094, PMCID: PMC2813755, DOI: 10.1038/sj.bjc.6605451.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnoikisCarcinoma, Squamous CellCell DivisionCell Line, TumorCell MovementCell TransdifferentiationDisease ProgressionFemaleGene Knockdown TechniquesHumansMiceMice, NudeNeoplasm InvasivenessNeoplasm ProteinsNeovascularization, PathologicReceptor, Notch1rho GTP-Binding ProteinsrhoC GTP-Binding ProteinRNA, Small InterferingSignal TransductionTumor Stem Cell AssayUterine Cervical NeoplasmsConceptsCervical carcinoma progressionRhoC protein expressionHuman cervical cancerCervical carcinoma cell linesPro-oncogenic roleHuman epithelial cancersNotch1 inactivationCervical cancerCarcinoma cell linesCervical carcinomaImmunohistochemical studyRole of NotchActive RhoCEpithelial cancersCarcinoma progressionSiHa cellsMesenchymal transitionNotch1 inhibitionWestern blottingProtein expressionClinical sectionTumor formationRhoC expressionColony formationTube formation
2000
Rapid induction of senescence in human cervical carcinoma cells
Goodwin E, Yang E, Lee C, Lee H, DiMaio D, Hwang E. Rapid induction of senescence in human cervical carcinoma cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 10978-10983. PMID: 11005870, PMCID: PMC27134, DOI: 10.1073/pnas.97.20.10978.Peer-Reviewed Original ResearchConceptsHuman papillomavirus E6Cervical carcinoma cellsCarcinoma cellsPapillomavirus E6Cervical carcinoma cell linesCell cycle regulatory proteinsViral oncogene expressionHuman cervical carcinoma cell lineCarcinoma cell linesCycle regulatory proteinsHuman cervical carcinoma cellsE7 oncogenesE7 proteinTransient alterationsCancer cellsOncogene expressionHuman cancersPhenotypic markersTelomerase activityCell linesRegulatory proteinsRapid inductionSenescence pathwaysCellsE2 regulatory protein
1999
Secretion of Vascular Endothelial Growth Factor in Adenocarcinoma and Squamous Cell Carcinoma of the Uterine Cervix
SANTIN A, HERMONAT P, RAVAGGI A, PECORELLI S, CANNON M, PARHAM G. Secretion of Vascular Endothelial Growth Factor in Adenocarcinoma and Squamous Cell Carcinoma of the Uterine Cervix. Obstetrics And Gynecology 1999, 94: 78-82. DOI: 10.1097/00006250-199907000-00015.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorSquamous cell carcinomaEndothelial growth factorVascular endothelial growth factor (VEGF) secretionSquamous cell linesUterine cervixCell carcinomaGrowth factor secretionGrowth factorFactor secretionCell linesSensitive enzyme-linked immunosorbentSignificant differencesEnzyme-linked immunosorbentCervical carcinoma cell linesImmunosuppressive cytokinesHistologic typeCervical cancerCarcinoma cell linesCervical tumorsBiologic behaviorHematogenous metastasisAdenocarcinomaCervixRadiation treatmentSecretion of vascular endothelial growth factor in adenocarcinoma and squamous cell carcinoma of the uterine cervix.
Santin A, Hermonat P, Ravaggi A, Pecorelli S, Cannon M, Parham G. Secretion of vascular endothelial growth factor in adenocarcinoma and squamous cell carcinoma of the uterine cervix. Obstetrics And Gynecology 1999, 94: 78-82. PMID: 10389722, DOI: 10.1016/s0029-7844(99)00282-3.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorSquamous cell carcinomaEndothelial growth factorVascular endothelial growth factor (VEGF) secretionSquamous cell linesUterine cervixCell carcinomaGrowth factor secretionGrowth factorFactor secretionCell linesSensitive enzyme-linked immunosorbentSignificant differencesEnzyme-linked immunosorbentCervical carcinoma cell linesImmunosuppressive cytokinesHistologic typeCervical cancerCarcinoma cell linesCervical tumorsBiologic behaviorHematogenous metastasisAdenocarcinomaCervixRadiation treatmentBovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein.
Naeger L, Goodwin E, Hwang E, DeFilippis R, Zhang H, DiMaio D. Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein. Molecular Cancer Research 1999, 10: 413-22. PMID: 10392903.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCattlecdc25 PhosphatasesCDC2-CDC28 KinasesCell Cycle ProteinsCell DivisionCyclin ACyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNA-Binding ProteinsE2F Transcription FactorsE2F1 Transcription FactorEnzyme InhibitorsFemaleGene Expression RegulationGrowth InhibitorsHeLa CellsHumansPhosphoprotein PhosphatasesPhosphorylationProtein Serine-Threonine KinasesProtein Tyrosine PhosphatasesRepressor ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Transcription Factor DP1Transcription FactorsTumor Cells, CulturedTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsCervical carcinoma cell linesHT-3 cellsCarcinoma cell linesE2 proteinE6/E7 genesCell linesGrowth inhibitionCervical carcinoma cellsCyclin ACyclin-dependent kinase 2 activityExpression of CDC25AKinase 2 activityE7 genesCell cycle componentsCarcinoma cellsCDC25B expressionE2 expressionProtein levels
1998
Radiation‐enhanced expression of E6/E7 transforming oncogenes of human papillomavirus‐16 in human cervical carcinoma
Santin A, Hermonat P, Ravaggi A, Chiriva‐Internati M, Pecorelli S, Parham G. Radiation‐enhanced expression of E6/E7 transforming oncogenes of human papillomavirus‐16 in human cervical carcinoma. Cancer 1998, 83: 2346-2352. PMID: 9840534, DOI: 10.1002/(sici)1097-0142(19981201)83:11<2346::aid-cncr14>3.0.co;2-g.Peer-Reviewed Original ResearchConceptsE6/E7 oncoproteinsMHC class ICervical carcinoma cell linesCarcinoma cell linesCervical carcinomaDose-dependent mannerE6/E7High dosesE7 oncoproteinsSiHa cell lineClass IRadiation therapyMajor histocompatibility complex restriction elementCell linesRadiation-induced drug resistanceHigh-risk HPV genotypesHPV-positive cervical carcinoma cell linesTumor cellsE6/E7 oncogenesE7 oncoprotein expressionE7 viral proteinsHuman papillomavirus infectionImportant risk factorTumor rejection antigensHuman papillomavirus 16Retinoic acid up-regulates the expression of major histocompatibility complex molecules and adhesion/costimulation molecules (specifically, intercellular adhesion molecule ICAM-1) in human cervical cancer
Santin A, Hermonat P, Ravaggi A, Chiriva-Internati M, Pecorelli S, Parham G. Retinoic acid up-regulates the expression of major histocompatibility complex molecules and adhesion/costimulation molecules (specifically, intercellular adhesion molecule ICAM-1) in human cervical cancer. American Journal Of Obstetrics And Gynecology 1998, 179: 1020-1025. PMID: 9790391, DOI: 10.1016/s0002-9378(98)70209-1.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernFemaleFlow CytometryFluorescent Antibody Technique, IndirectGene Expression Regulation, NeoplasticHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansIntercellular Adhesion Molecule-1RNA, MessengerTretinoinTumor Cells, CulturedUterine Cervical NeoplasmsConceptsMajor histocompatibility complex class IHistocompatibility complex class IComplex class IICAM-1 antigenCervical cancerCervical carcinoma cell linesSurface antigenClass IHuman cervical carcinoma cell lineRetinoic acidCarcinoma cell linesTherapeutic dosesHuman leukocyte antigen (HLA) class IClass II surface antigensCell linesAdhesion molecules ICAM-1Fluorescence-activated cell sorter analysisIntercellular adhesion molecule ICAM-1Additional clinical researchHuman leukocyte antigenMajor histocompatibility complex moleculesAntigen class IMolecules ICAM-1Use of retinoidsUntreated tumor cellsTransactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines
Goodwin E, Naeger L, Breiding D, Androphy E, DiMaio D. Transactivation-Competent Bovine Papillomavirus E2 Protein Is Specifically Required for Efficient Repression of Human Papillomavirus Oncogene Expression and for Acute Growth Inhibition of Cervical Carcinoma Cell Lines. Journal Of Virology 1998, 72: 3925-3934. PMID: 9557678, PMCID: PMC109618, DOI: 10.1128/jvi.72.5.3925-3934.1998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesBovine papillomavirus 1CattleCell DivisionCell NucleusCOS CellsDNADNA-Binding ProteinsFemaleGene Expression Regulation, ViralHeLa CellsHumansMutagenesisOncogene Proteins, ViralOncogenesPapillomaviridaeRepressor ProteinsRNA, MessengerRNA, ViralTrans-ActivatorsTranscriptional ActivationTumor Cells, CulturedUterine Cervical NeoplasmsViral ProteinsConceptsPapillomavirus E2 proteinGrowth arrestHT-3 cellsEfficient repressionTransactivation domainE2 proteinHeLa cellsG1/S-phase growth arrestE2 mutantsBovine papillomavirus type 1 E2 proteinBovine papillomavirus E2 proteinHerpes simplex virus VP16Reporter plasmidAcute growth inhibitionE2 transactivation domainGrowth inhibitionCervical carcinoma cell linesBPV1 E2 proteinCarcinoma cell linesHuman papillomavirus oncogene expressionViral DNA replicationPhase growth arrestSequence-specific transactivatorCell linesWild-type p53 geneEffects of retinoic acid combined with interferon‐gamma on the expression of major‐histocompatibility‐complex molecules and intercellular adhesion molecule‐1 in human cervical cancer
Santin A, Hermonat P, Ravaggi A, Chiriva‐Internati M, Hiserodt J, Tian E, Carter C, Pecorelli S, Parham G. Effects of retinoic acid combined with interferon‐gamma on the expression of major‐histocompatibility‐complex molecules and intercellular adhesion molecule‐1 in human cervical cancer. International Journal Of Cancer 1998, 75: 254-258. PMID: 9462716, DOI: 10.1002/(sici)1097-0215(19980119)75:2<254::aid-ijc14>3.0.co;2-d.Peer-Reviewed Original ResearchConceptsMHC class IIIFN-gammaHuman cervical cancerCervical cancerHLA classTrans retinoic acidMHC classI mRNA expressionRetinoic acidSurface antigenTumor cellsIntercellular adhesion molecule-1Biologic response modifiersICAM-1 antigenAdhesion molecule-1ICAM-1 moleculesUntreated tumor cellsCell linesCervical cancer cellsICAM-1 surfaceCervical carcinoma cell linesAnti-proliferative effectsHuman cervical cancer cellsHuman cervical carcinoma cell lineResponse modifiersEffects of retinoic acid on the expression of a tumor rejection antigen (heat shock protein gp96) in human cervical cancer.
Santin AD, Hermonat PL, Ravaggi A, Chiriva-Internati M, Pecorelli S, Parham GP. Effects of retinoic acid on the expression of a tumor rejection antigen (heat shock protein gp96) in human cervical cancer. European Journal Of Gynaecological Oncology 1998, 19: 229-33. PMID: 9641219.Peer-Reviewed Original ResearchConceptsHeat shock protein gp96Shock protein gp96Tumor rejection antigensHuman cervical cancerCervical cancerRetinoic acidRejection antigensTumor cellsMajor histocompatibility complex class IPresentation of tumorHistocompatibility complex class IBiologic response modifiersCervical cancer cell linesCo-stimulation moleculesComplex class ICell linesCervical carcinoma cell linesHuman cervical carcinoma cell lineCancer cell linesCarcinoma cell linesHumoral immunityTherapeutic dosesViral antigensResponse modifiersICAM-1The effects of irradiation on the expression of a tumour rejection antigen (heat shock protein gp96) in human cervical cancer
Santin AD, Hermonat PL, Ravaggi A, Chiriva-Internati M, Hiserodt JC, Batchu RB, Pecorelli S, Parham GP. The effects of irradiation on the expression of a tumour rejection antigen (heat shock protein gp96) in human cervical cancer. International Journal Of Radiation Biology 1998, 73: 699-704. PMID: 9690688, DOI: 10.1080/095530098141951.Peer-Reviewed Original ResearchConceptsTumor rejection antigensHeat shock protein gp96Shock protein gp96Human cervical cancerCervical carcinoma cell linesRejection antigensHigh dosesHuman cervical carcinoma cell lineCervical cancerCarcinoma cell linesTumor cellsMajor histocompatibility complex class ILocal radiation therapyHistocompatibility complex class IBiologic response modifiersCo-stimulation moleculesComplex class ICell linesCervical cancer cellsDose-dependent mannerViral antigensResponse modifiersICAM-1Radiation therapyGp96 mRNA
1997
Effects of irradiation on the expression of major histocompatibility complex class I antigen and adhesion costimulation molecules ICAM-1 in human cervical cancer
Santin A, Hermonat P, Hiserodt J, Chiriva-Internati M, Woodliff J, Theus J, Barclay D, Pecorelli S, Parham G. Effects of irradiation on the expression of major histocompatibility complex class I antigen and adhesion costimulation molecules ICAM-1 in human cervical cancer. International Journal Of Radiation Oncology • Biology • Physics 1997, 39: 737-742. PMID: 9336157, DOI: 10.1016/s0360-3016(97)00372-6.Peer-Reviewed Original ResearchConceptsMHC class ISurface antigenClass IAntigen expressionICAM-1High dosesClass II surface antigensCell linesSiHa cervical cancer cellsMajor histocompatibility complex class IHistocompatibility complex class IICAM-1 antigenMolecules ICAM-1Cell replicationSurface antigen expressionHuman cervical cancerComplex class ICervical cancer cellsCervical carcinoma cell linesCervical cell linesHuman cervical carcinoma cell lineDifferent time pointsCervical cancerCarcinoma cell linesHigh radiation doses
1993
Inhibition of cervical carcinoma cell line proliferation by the introduction of a bovine papillomavirus regulatory gene
Hwang E, Riese D, Settleman J, Nilson L, Honig J, Flynn S, DiMaio D. Inhibition of cervical carcinoma cell line proliferation by the introduction of a bovine papillomavirus regulatory gene. Journal Of Virology 1993, 67: 3720-3729. PMID: 8389903, PMCID: PMC237735, DOI: 10.1128/jvi.67.7.3720-3729.1993.Peer-Reviewed Original ResearchConceptsBPV E2 proteinGene expressionCervical carcinoma cell linesRegulatory genesHPV gene expressionCarcinoma cell linesCell linesE2 proteinHeLa cellsConsequence of abrogationP53 tumor suppressor proteinHPV18 E6Tumor suppressor proteinE2 geneDestabilization of p53HT-3 cellsGrowth inhibitionBPV E2Simian virus 40Suppressor proteinEpithelial cell lineHuman papillomavirus E6Cell cycleGenesS phase
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