2018
Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
Ravi B, Nassar LM, Kopchock RJ, Dhakal P, Scheetz M, Collins KM. Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans. Journal Of Visualized Experiments 2018, 56911. PMID: 29443112, PMCID: PMC5912386, DOI: 10.3791/56911.Peer-Reviewed Original ResearchConceptsHermaphrodite-specific neuronsEgg-laying circuitCaenorhabditis elegans wormsEgg-laying behaviorNon-invasive optical approachSerotonergic Hermaphrodite Specific NeuronsCaenorhabditis elegansMolecular mechanismsFluorescent reportersFluorescent proteinSensitive CaIntracellular CaNeural circuit activityRatiometric CaSpecific neuronsDifferent behavior statesRatiometric calcium imagingAnimal trackingBrightfield imagingCircuit activitySynaptic activityElegansGlass coverslipsWormsCells
2017
Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin?
Henderson D, Huebner C, Markowitz M, Taube N, Harvanek ZM, Jakob U, Knoefler D. Do developmental temperatures affect redox level and lifespan in C. elegans through upregulation of peroxiredoxin? Redox Biology 2017, 14: 386-390. PMID: 29055282, PMCID: PMC5647470, DOI: 10.1016/j.redox.2017.10.003.Peer-Reviewed Original ResearchConceptsC. elegansC. elegans larvaeRedox stateVivo redox stateReduced redox statePoikilothermic organismsDevelopmental temperatureLifespan extensionRedox statusMechanistic underpinningsBeneficial long-term consequencesElegansExpression levelsOrganismsVivo redox statusWormsRedox levelsCaenorhabditisLifespanPeroxiredoxinsGrowth temperatureLarvaeAnimalsUpregulationEffect of temperatureUbiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange During Spermiogenesis
Gou L, Kang J, Dai P, Wang X, Li F, Zhao S, Zhang M, Hua M, Lu Y, Zhu Y, Li Z, Chen H, Wu L, Li D, Fu X, Li J, Shi H, Liu M. Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange During Spermiogenesis. Obstetrical & Gynecological Survey 2017, 72: 540-541. DOI: 10.1097/ogx.0000000000000482.Peer-Reviewed Original ResearchGenome-Wide RNA Interference: Functional Genomics in the Postgenomics Era.
Politi K, Wajapeyee N. Genome-Wide RNA Interference: Functional Genomics in the Postgenomics Era. Cold Spring Harbor Protocols 2017, 2017: pdb.top097550. PMID: 28864574, DOI: 10.1101/pdb.top097550.Chapters
2014
Comparative analysis of regulatory information and circuits across distant species
Boyle AP, Araya CL, Brdlik C, Cayting P, Cheng C, Cheng Y, Gardner K, Hillier LW, Janette J, Jiang L, Kasper D, Kawli T, Kheradpour P, Kundaje A, Li JJ, Ma L, Niu W, Rehm EJ, Rozowsky J, Slattery M, Spokony R, Terrell R, Vafeados D, Wang D, Weisdepp P, Wu YC, Xie D, Yan KK, Feingold EA, Good PJ, Pazin MJ, Huang H, Bickel PJ, Brenner SE, Reinke V, Waterston RH, Gerstein M, White KP, Kellis M, Snyder M. Comparative analysis of regulatory information and circuits across distant species. Nature 2014, 512: 453-456. PMID: 25164757, PMCID: PMC4336544, DOI: 10.1038/nature13668.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCaenorhabditis elegansChromatin ImmunoprecipitationConserved SequenceDrosophila melanogasterEvolution, MolecularGene Expression RegulationGene Expression Regulation, DevelopmentalGene Regulatory NetworksGenomeHumansMolecular Sequence AnnotationNucleotide MotifsOrgan SpecificityTranscription FactorsComparative analysis of the transcriptome across distant species
Gerstein MB, Rozowsky J, Yan KK, Wang D, Cheng C, Brown JB, Davis CA, Hillier L, Sisu C, Li JJ, Pei B, Harmanci AO, Duff MO, Djebali S, Alexander RP, Alver BH, Auerbach R, Bell K, Bickel PJ, Boeck ME, Boley NP, Booth BW, Cherbas L, Cherbas P, Di C, Dobin A, Drenkow J, Ewing B, Fang G, Fastuca M, Feingold EA, Frankish A, Gao G, Good PJ, Guigó R, Hammonds A, Harrow J, Hoskins RA, Howald C, Hu L, Huang H, Hubbard TJ, Huynh C, Jha S, Kasper D, Kato M, Kaufman TC, Kitchen RR, Ladewig E, Lagarde J, Lai E, Leng J, Lu Z, MacCoss M, May G, McWhirter R, Merrihew G, Miller DM, Mortazavi A, Murad R, Oliver B, Olson S, Park PJ, Pazin MJ, Perrimon N, Pervouchine D, Reinke V, Reymond A, Robinson G, Samsonova A, Saunders GI, Schlesinger F, Sethi A, Slack FJ, Spencer WC, Stoiber MH, Strasbourger P, Tanzer A, Thompson OA, Wan KH, Wang G, Wang H, Watkins KL, Wen J, Wen K, Xue C, Yang L, Yip K, Zaleski C, Zhang Y, Zheng H, Brenner SE, Graveley BR, Celniker SE, Gingeras TR, Waterston R. Comparative analysis of the transcriptome across distant species. Nature 2014, 512: 445-448. PMID: 25164755, PMCID: PMC4155737, DOI: 10.1038/nature13424.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansChromatinCluster AnalysisDrosophila melanogasterGene Expression ProfilingGene Expression Regulation, DevelopmentalHistonesHumansLarvaModels, GeneticMolecular Sequence AnnotationPromoter Regions, GeneticPupaRNA, UntranslatedSequence Analysis, RNATranscriptome
2005
Unsuccessful search for DNA transfer from transgenic plants to bacteria in the intestine of the tobacco horn worm, Manduca sexta
Deni J, Message B, Chioccioli M, Tepfer D. Unsuccessful search for DNA transfer from transgenic plants to bacteria in the intestine of the tobacco horn worm, Manduca sexta. Transgenic Research 2005, 14: 207-215. PMID: 16022391, DOI: 10.1007/s11248-004-6701-z.Peer-Reviewed Original ResearchConceptsTobacco horn wormTransgenic plantsDNA transferIntact leaf cellsKanamycin resistance geneLeaf cellsNptII geneHeat-labile activityResistance genesNative bacteriaGene transferTransfer of fragmentsExchange assayDNAGenesBacteriaPlantsIntestinal bacteriaNptIIAcinetobacterWormsChloroplastsGutBD413Manduca
2004
Identification of 315 genes essential for early zebrafish development
Amsterdam A, Nissen R, Sun Z, Swindell E, Farrington S, Hopkins N. Identification of 315 genes essential for early zebrafish development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 12792-12797. PMID: 15256591, PMCID: PMC516474, DOI: 10.1073/pnas.0403929101.Peer-Reviewed Original ResearchConceptsZebrafish developmentOrthologues of genesInsertional mutagenesis screenMutant collectionFish genesEssential genesMutagenesis screenEarly larval developmentYeastGenesMutantsDevelopmental biologyStudies of cellsDevelopmental defectsEmbryonic developmentHomologuesLarval developmentCell typesIndividual organsCellsInvertebratesRe-screeningWormsMutationsGenetics
2001
The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling
Salcini A, Hilliard M, Croce A, Arbucci S, Luzzi P, Tacchetti C, Daniell L, De Camilli P, Pelicci P, Di Fiore P, Bazzicalupo P. The Eps15 C. elegans homologue EHS-1 is implicated in synaptic vesicle recycling. Nature Cell Biology 2001, 3: 755-760. PMID: 11483962, DOI: 10.1038/35087075.Peer-Reviewed Original ResearchMeSH KeywordsAldicarbAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCalcium-Binding ProteinsDynaminsFluorescent Antibody TechniqueGanglia, InvertebrateGene DeletionGenes, ReporterGTP PhosphohydrolasesInsecticidesMicroscopy, ElectronMolecular Sequence DataMovement DisordersMutationNerve Tissue ProteinsNervous SystemPhenotypePhosphoproteinsProtein TransportSequence Homology, Nucleic AcidSynaptic VesiclesTemperatureConceptsSynaptic vesicle recyclingVesicle recyclingEHS-1Protein-protein interactionsMammalian Eps15Dynamin proteinsEH domainEndocytic machineryEps15Mutant formsPermissive temperatureFunctional studiesSynaptic vesiclesDynaminUncoordinated movementsPresynaptic defectsProteinPhenotypeOrthologuesCaenorhabditisWormsGenesNematodesMachineryVesicles
1989
Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
Reis M, Kuhns J, Blanton R, Davis A. Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular And Biochemical Parasitology 1989, 32: 113-119. PMID: 2927441, DOI: 10.1016/0166-6851(89)90062-5.Peer-Reviewed Original ResearchConceptsSex-specific gene expressionFemale-specific mRNAOpen reading frameFunction of eggsPattern of expressionMature female wormsNucleotide mRNACDNA sequenceReading frameGene expressionSpecific mRNAsFemale wormsFs800Central stretchAmino acidsVitelline cellsSitu hybridizationGenesMRNASchistosoma mansoniEgg productionExpressionWormsSequenceCDNA
1987
Lack of demonstrable density-dependent fecundity of schistosomiasis mansoni: analyses of Egyptian quantitative human autopsies.
Wertheimer S, Vermund S, Lumey L, Singer B. Lack of demonstrable density-dependent fecundity of schistosomiasis mansoni: analyses of Egyptian quantitative human autopsies. American Journal Of Tropical Medicine And Hygiene 1987, 37: 79-84. PMID: 3111282, DOI: 10.4269/ajtmh.1987.37.79.Peer-Reviewed Original ResearchConceptsFemale wormsActive Schistosoma mansoni infectionSchistosoma mansoni infectionMansoni infectionSchistosomiasis mansoniHuman autopsiesWorm fecundityWorm burdenS. mansoniExploratory regression analysisHuman hostRegression analysisNumber of eggsAutopsiesMansoniFecesHost tissuesTissueEggs/InfectionWorms
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