2024
Electron tomography visualization of HIV-1 virions trapped by fusion inhibitors to host cells in infected tissues
Ladinsky M, Zhu L, Ullah I, Uchil P, Kumar P, Kay M, Bjorkman P. Electron tomography visualization of HIV-1 virions trapped by fusion inhibitors to host cells in infected tissues. Journal Of Virology 2024, 98: e01432-24. PMID: 39475277, PMCID: PMC11575291, DOI: 10.1128/jvi.01432-24.Peer-Reviewed Original ResearchHIV-1 virionsHIV-1 target cellsHIV-1Fusion inhibitorsTarget cellsBone marrow/liver/thymus miceHIV-1 pseudovirionsCo-receptorHost cell membraneTZM-bl cellsPre-hairpin intermediateVascular endothelial cellsCell surfaceHumanized miceTZM-blCell membraneInfected tissuesEnvEndothelial cellsViral envelopeBind host receptorsHost receptorsInhibitorsCo-receptor proteinsPresence of virions
2023
Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry
Leonhardt S, Purdy M, Grover J, Yang Z, Poulos S, McIntire W, Tatham E, Erramilli S, Nosol K, Lai K, Ding S, Lu M, Uchil P, Finzi A, Rein A, Kossiakoff A, Mothes W, Yeager M. Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nature Communications 2023, 14: 4368. PMID: 37474505, PMCID: PMC10359404, DOI: 10.1038/s41467-023-39262-2.Peer-Reviewed Original Research
2021
Modulating HIV-1 envelope glycoprotein conformation to decrease the HIV-1 reservoir
Rajashekar JK, Richard J, Beloor J, Prévost J, Anand SP, Beaudoin-Bussières G, Shan L, Herndler-Brandstetter D, Gendron-Lepage G, Medjahed H, Bourassa C, Gaudette F, Ullah I, Symmes K, Peric A, Lindemuth E, Bibollet-Ruche F, Park J, Chen HC, Kaufmann DE, Hahn BH, Sodroski J, Pazgier M, Flavell RA, Smith AB, Finzi A, Kumar P. Modulating HIV-1 envelope glycoprotein conformation to decrease the HIV-1 reservoir. Cell Host & Microbe 2021, 29: 904-916.e6. PMID: 34019804, PMCID: PMC8214472, DOI: 10.1016/j.chom.2021.04.014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, NeutralizingAntibody-Dependent Cell CytotoxicityAntiviral AgentsCD4 AntigensCD4-Positive T-LymphocytesCell Lineenv Gene Products, Human Immunodeficiency VirusEpitopesFemaleGlycoproteinsHEK293 CellsHIV InfectionsHIV-1HumansImmunoglobulin Fc FragmentsKiller Cells, NaturalMaleMiceMice, SCIDModels, AnimalProtein ConformationVirus ReplicationConceptsAntibody-dependent cellular cytotoxicityHIV-1 reservoirFc effector functionsViral reboundHumanized miceHIV-1HIV-1-infected individualsHIV-1-infected cellsAutologous HIV-1Natural killer cellsCD4-mimetic compoundsHIV-1 replicationSmall CD4-mimetic compoundsART interruptionFunctional cureNK cellsKiller cellsCellular cytotoxicityTherapeutic utilityInfected individualsCD4mcVirus reservoirMiceViral envelopeAntibody recognition
2017
Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes
Chandra S, Kalaivani R, Kumar M, Srinivasan N, Sarkar D. Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes. Molecular Biology Of The Cell 2017, 28: 3801-3814. PMID: 29074568, PMCID: PMC5739296, DOI: 10.1091/mbc.e17-06-0400.Peer-Reviewed Original ResearchConceptsMembrane fusionQuantitative mass spectrometryTwo-dimensional differentialViral envelope glycoproteinsSendai viral envelopesEarly molecular eventsHost cell proteinsActin cytoskeletonThreonine 206Actin dynamicsSendai virusAnimal cellsMolecular eventsCellular membranesHost cellsCell proteinsFusion-mediated deliveryNovel mechanismBiochemical analysisVillinEnvelope glycoproteinGel electrophoresisViral envelopeProteinViral entry
2015
Membrane Fusion Mediated Targeted Cytosolic Drug Delivery Through scFv Engineered Sendai Viral Envelopes.
Kumar M, Mani P, Pratheesh P, Chandra S, Jeyakkodi M, Chattopadhyay P, Sarkar D, Sinha S. Membrane Fusion Mediated Targeted Cytosolic Drug Delivery Through scFv Engineered Sendai Viral Envelopes. 2015, 15: 386-400. PMID: 25941820, DOI: 10.2174/1566524015666150505155949.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAntibiotics, AntineoplasticAntibodies, MonoclonalCell Line, TumorCHO CellsCricetinaeCricetulusCytoplasmDoxorubicinDrug Delivery SystemsFluorescein-5-isothiocyanateGPI-Linked ProteinsHeLa CellsHumansIsoenzymesMembrane FusionPeptide LibrarySendai virusStaining and LabelingViral Envelope ProteinsViral Fusion ProteinsConceptsOnco-fetal antigenF proteinNumber of malignanciesNative F proteinVariety of cancersAlkaline phosphataseViral envelopeCytoplasmic deliveryBreast cancerSendai viral envelopesSpecific antibodiesPAPPlacental isozymeCancerSpecific bindingAntibodiesDrugsDeliveryCytosolic drug deliveryPotential usefulnessRecombinant scFvReduced degradationHN proteinUbiquitous expression
1985
Effects of monovalent cations on Semliki Forest virus entry into BHK-21 cells.
Helenius A, Kielian M, Wellsteed J, Mellman I, Rudnick G. Effects of monovalent cations on Semliki Forest virus entry into BHK-21 cells. Journal Of Biological Chemistry 1985, 260: 5691-5697. PMID: 3988769, DOI: 10.1016/s0021-9258(18)89078-0.Peer-Reviewed Original ResearchConceptsSemliki Forest virusEndosome membraneViral envelopeBaby hamster kidney cellsMammalian cellsEndosomal membranesLow endosomalBHK-21 cellsHamster kidney cellsVirus endocytosisIntact cellsConformational changesEndosomesPrelysosomal endosomesViral spike glycoproteinVirus fusionKidney cellsAcidic endosomal pHViral RNAVoltage-sensitive probeEndocytosisVirus entryEndosomal pHCellsSpike glycoprotein
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