2018
High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis
Ramakrishnan S, Gohlke A, Li F, Coleman J, Xu W, Rothman JE, Pincet F. High-Throughput Monitoring of Single Vesicle Fusion Using Freestanding Membranes and Automated Analysis. Langmuir 2018, 34: 5849-5859. PMID: 29694054, DOI: 10.1021/acs.langmuir.8b00116.Peer-Reviewed Original ResearchConceptsMembrane fusionFusion eventsSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsSNARE-dependent membrane fusionIndividual vesicle fusion eventsFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsT-SNARE proteinsSingle-vesicle fusionProtein receptor proteinsVesicle fusion eventsMobility of proteinsVesicle dockingContent releaseVesicle fusionHigh-throughput monitoringPlanar membranesReceptor proteinLipid mixingProteinLipid bilayersVesiclesCorrect reconstitutionMembraneAqueous compartment
2012
The Legionella pneumophila Effector DrrA Is Sufficient to Stimulate SNARE-Dependent Membrane Fusion
Arasaki K, Toomre DK, Roy CR. The Legionella pneumophila Effector DrrA Is Sufficient to Stimulate SNARE-Dependent Membrane Fusion. Cell Host & Microbe 2012, 11: 46-57. PMID: 22264512, PMCID: PMC3266541, DOI: 10.1016/j.chom.2011.11.009.Peer-Reviewed Original ResearchConceptsMembrane transport pathwaysEndoplasmic reticulumSyntaxin proteinsFusion of ERMembrane fusionSNARE-dependent membrane fusionBacterial pathogen Legionella pneumophilaPathogen-containing vacuolesSNARE protein Sec22bIntracellular bacterial pathogen Legionella pneumophilaPathogen Legionella pneumophilaFusion of vesiclesRab1 activationNoncanonical pairingTransport pathwaysRab1 GTPasePlasma membraneVesicle fusionOrganellesDrrASec22bVesiclesLegionella pneumophilaProteinVacuoles
1999
The Length of the Flexible SNAREpin Juxtamembrane Region Is a Critical Determinant of SNARE-Dependent Fusion
McNew J, Weber T, Engelman D, Söllner T, Rothman J. The Length of the Flexible SNAREpin Juxtamembrane Region Is a Critical Determinant of SNARE-Dependent Fusion. Molecular Cell 1999, 4: 415-421. PMID: 10518222, DOI: 10.1016/s1097-2765(00)80343-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAntigens, SurfaceCarrier ProteinsMembrane FusionMembrane ProteinsMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsPliabilityProlineProtein Structure, SecondaryRecombinant ProteinsR-SNARE ProteinsSNARE ProteinsSynaptosomal-Associated Protein 25Syntaxin 1Vesicular Transport ProteinsConceptsJuxtamembrane regionMembrane fusionSNARE-dependent membrane fusionSNARE-dependent fusionHelix-breaking proline residueSNARE proteinsTransmembrane domainSyntaxin 1ACoil domainProline residuesFlexible linkerLipid bilayersCritical determinantFusion efficiencyFusionVAMPDomainProteinRate of fusionSnareVesiclesResiduesLinkerSame changesRegion
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