2025
Alternative Splicing Alterations in Patients With Amyotrophic Lateral Sclerosis: Link to the Disruption of TAR DNA‐Binding Protein 43 kDa Functions
Miwa T, Takeuchi E, Ogawa K, Abdelhamid R, Morita J, Hiraki Y, Yasumizu Y, Nakamura Y, Ohkura N, Saito Y, Murayama S, Nagai Y, Mochizuki H, Nagano S. Alternative Splicing Alterations in Patients With Amyotrophic Lateral Sclerosis: Link to the Disruption of TAR DNA‐Binding Protein 43 kDa Functions. Neurology And Clinical Neuroscience 2025, 13: 187-194. DOI: 10.1111/ncn3.12880.Peer-Reviewed Original ResearchAlternative splicing alterationsAlternative splicingTDP-43Splicing changesSplicing alterationsAmyotrophic lateral sclerosis pathologyAmyotrophic lateral sclerosisDNA-binding proteinsDysregulation of alternative splicingTAR DNA-binding proteinAberrant alternative splicingTAR DNA-binding protein 43 kDaSH-SY5Y cellsRNA metabolismDNA-binding protein 43 kDaNovel genesSplicing patternsNeurons of patientsRNA sequencingSplicingLateral sclerosisMotor neuronsTreatment of amyotrophic lateral sclerosisPolymerase chain reaction analysisMotor neurons of patients
2024
Acatulides A-G, neuroprotective macrolides from Acaulium album H-JQSF
Tong Z, Wang T, Yang P, Sun J, Zhang C, Khan S, Wang X, Jiao R, Ge H, Zhuang W, Hu G, Tan R. Acatulides A-G, neuroprotective macrolides from Acaulium album H-JQSF. Chinese Chemical Letters 2024, 35: 108488. DOI: 10.1016/j.cclet.2023.108488.Peer-Reviewed Original Research
2023
Cellular protection from H2O2 toxicity by Fv-Hsp70: protection via catalase and gamma-glutamyl-cysteine synthase
Hino C, Chan G, Jordaan G, Chang S, Saunders J, Bashir M, Hansen J, Gera J, Weisbart R, Nishimura R. Cellular protection from H2O2 toxicity by Fv-Hsp70: protection via catalase and gamma-glutamyl-cysteine synthase. Cell Stress And Chaperones 2023, 28: 429-439. PMID: 37171750, PMCID: PMC10352194, DOI: 10.1007/s12192-023-01349-6.Peer-Reviewed Original ResearchConceptsHeat shock proteinsGamma-glutamylcysteine synthaseCellular protectionAcute oxidative stressLid domainProtein-binding domainsATPase domainCysteine synthaseOxidative stressCellular stressRecombinant proteinsSH-SY5Y cellsEndogenous heat shock proteinsShock proteinsCell deathProteinProtein catalaseHSP70H2O2 toxicityCellsSynthaseΜM concentrationsDomainCatalaseDirect response
2013
Glutaredoxin 1 is a major player in copper metabolism in neuroblastoma cells
De Benedetto ML, Capo CR, Ferri A, Valle C, Polimanti R, Carrì MT, Rossi L. Glutaredoxin 1 is a major player in copper metabolism in neuroblastoma cells. Biochimica Et Biophysica Acta 2013, 1840: 255-261. PMID: 24041990, DOI: 10.1016/j.bbagen.2013.09.008.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBlotting, WesternCation Transport ProteinsCell ProliferationChromatography, AffinityCopperCopper Transporter 1GlutaredoxinsGlutathioneHumansMitochondriaNeuroblastomaOxidation-ReductionReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSuperoxide DismutaseSuperoxide Dismutase-1Tumor Cells, CulturedConceptsIntracellular copper levelsCopper transporter 1Copper-induced toxicityHomeostasis of copperCopper metabolismCopper-binding propertiesGRX1 overexpressionProtein chaperonesHuman neuroblastoma SH-SY5Y cellsCopper overloadCopper chaperoneMajor playersNeuroblastoma SH-SY5Y cellsThioredoxin familyProtein disulfidesGrx1Glutaredoxin-1SH-SY5Y cellsSmall proteinsCuSO4 treatmentNeuronal cellsMixed disulfidesChaperonesControl cellsEnzyme activity
2010
Effect of Trehalose on the Properties of Mutant γPKC, Which Causes Spinocerebellar Ataxia Type 14, in Neuronal Cell Lines and Cultured Purkinje Cells*
Seki T, Abe-Seki N, Kikawada T, Takahashi H, Yamamoto K, Adachi N, Tanaka S, Hide I, Saito N, Sakai N. Effect of Trehalose on the Properties of Mutant γPKC, Which Causes Spinocerebellar Ataxia Type 14, in Neuronal Cell Lines and Cultured Purkinje Cells*. Journal Of Biological Chemistry 2010, 285: 33252-33264. PMID: 20705605, PMCID: PMC2963337, DOI: 10.1074/jbc.m110.146704.Peer-Reviewed Original ResearchConceptsPrimary cultured Purkinje cellsApoptotic cell deathSH-SY5Y cellsNeuronal cell lineCell deathDevelopment of dendritesSH-SY5YSpinocerebellar ataxia type 14Autosomal dominant neurodegenerative diseaseCell linesDominant neurodegenerative diseaseModel of Huntington's diseaseSusceptible to aggregationAnimal models of Huntington's diseaseMissense mutationsPrion diseasesHuntington's diseaseProtein turnoverType 14Spinocerebellar ataxia typeDisaccharide trehaloseAlzheimer's diseaseNeurodegenerative diseasesCellular dysfunctionTrehaloseHaloperidol disrupts lipid rafts and impairs insulin signaling in SH-SY5Y cells
Sánchez-Wandelmer J, Dávalos A, de la Peña G, Cano S, Giera M, Canfrán-Duque A, Bracher F, Martín-Hidalgo A, Fernández-Hernando C, Lasunción M, Busto R. Haloperidol disrupts lipid rafts and impairs insulin signaling in SH-SY5Y cells. Neuroscience 2010, 167: 143-153. PMID: 20123000, DOI: 10.1016/j.neuroscience.2010.01.051.Peer-Reviewed Original ResearchConceptsEffects of haloperidolCholesterol contentLipid raft compositionCholesterol biosynthesisCell cholesterol contentSH-SY5Y cellsNeuroblastoma SH-SY5YHL-60 human cell linesDopamine receptorsMetabolic effectsTherapeutic effectHaloperidolInsulin-Akt signalingImpairs insulinCellular cholesterol homeostasisLipid raftsSH-SY5YFree cholesterolCholesterol homeostasisDependent insulinDrug inhibitionHuman cell linesCell linesPhysiological vehicleInsulinCongo Red, an Amyloid-Inhibiting Compound, Alleviates Various Types of Cellular Dysfunction Triggered by Mutant Protein Kinase Cγ That Causes Spinocerebellar Ataxia Type 14 (SCA14) by Inhibiting Oligomerization and Aggregation
Seki T, Takahashi H, Yamamoto K, Ogawa K, Onji T, Adachi N, Tanaka S, Hide I, Saito N, Sakai N. Congo Red, an Amyloid-Inhibiting Compound, Alleviates Various Types of Cellular Dysfunction Triggered by Mutant Protein Kinase Cγ That Causes Spinocerebellar Ataxia Type 14 (SCA14) by Inhibiting Oligomerization and Aggregation. Journal Of Pharmacological Sciences 2010, 114: 206. PMID: 20938103, DOI: 10.1254/jphs.10170fp.Peer-Reviewed Original ResearchMeSH KeywordsAmyloidAnimalsApoptosisCells, CulturedCerebellumColoring AgentsCongo RedDendritesDose-Response Relationship, DrugEmbryo, MammalianHumansMiceMice, Inbred ICRMutant ProteinsMutation, MissenseNeuroblastomaNeurodegenerative DiseasesProtein Kinase CPurkinje CellsSpinocerebellar AtaxiasSpinocerebellar DegenerationsConceptsGreen fluorescent proteinApoptotic cell deathCell deathFibril formationFormation of misfolded proteinsSpinocerebellar ataxia type 14Autosomal dominant neurodegenerative diseaseDominant neurodegenerative diseaseAggregate formationSH-SY5Y cellsAmyloid oligomersCongo redSusceptible to aggregationMissense mutationsFluorescent proteinType 14SH-SY5YDevelopment of dendritesSCA14Spinocerebellar ataxia typeNeurodegenerative diseasesHistological dyesCellular dysfunctionExpression levelsProtein
2006
Hemodynamic Effects of Di-Sec-Butyl Phenol, an Anesthetic Substituted Phenol
Deau J, Heerdt PM, H.S. The A, Wang Q. Hemodynamic Effects of Di-Sec-Butyl Phenol, an Anesthetic Substituted Phenol. Pharmacology 2006, 76: 117-122. PMID: 16374073, DOI: 10.1159/000090501.Peer-Reviewed Original ResearchConceptsMean blood pressureBlood pressureHeart rateHemodynamic effectsSympathetic nervous systemAge-dependent elevationHuman neuroblastoma cell lineRelease of catecholaminesSH-SY5Y cellsNeuroblastoma cell linesGanglionic blockadeNorepinephrine releaseNervous systemControl valuesYoung rabbitsCell linesElderly rabbitsRabbitsHypnosisHexamethoniumBlockadeCatecholaminesDoseRelease
2000
Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis
Nath R, Huggins M, Glantz S, Morrow J, McGinnis K, Nadimpalli R, Wang K. Development and characterization of antibodies specific to caspase-3-produced alpha II-spectrin 120 kDa breakdown product: marker for neuronal apoptosis. Neurochemistry International 2000, 37: 351-361. PMID: 10825575, DOI: 10.1016/s0197-0186(00)00040-1.Peer-Reviewed Original ResearchConceptsWestern blotRat cerebellar granule neuronsHuman neuroblastoma SH-SY5Y cellsNeuroblastoma SH-SY5Y cellsSpectrin breakdown productsCerebellar granule neuronsSH-SY5Y cellsApoptotic neuronsCharacterization of antibodiesNeuronal apoptosisNeurodegenerative conditionsGranule neuronsBreakdown productsImmunocytochemical studySH-SY5YII-spectrinWithdrawal-induced apoptosisAntibodiesNeuronsCaspase-3Apoptotic deathPowerful markerChicken antibodiesApoptosisAlpha-spectrin
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