2019
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, Li J, Genet N, Henrique Geraldo L, Benedetti L, Künzel S, Pibouin-Fragner L, Thomas JL, Eichmann A. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis. Nature Communications 2019, 10: 2350. PMID: 31138815, PMCID: PMC6538628, DOI: 10.1038/s41467-019-10359-x.Peer-Reviewed Original ResearchMeSH KeywordsAcyltransferasesAnimalsCell MovementCell PolarityCell ProliferationCell SurvivalEndocytosisEndothelial CellsIntercellular Signaling Peptides and ProteinsMAP Kinase Signaling SystemMiceMice, KnockoutNeovascularization, PhysiologicNerve Tissue Proteinsp21-Activated KinasesReceptors, ImmunologicRetinal VesselsVascular Endothelial Growth Factor Receptor-2ConceptsEndophilin A2Endothelial cell migrationSprouting angiogenesisCell migrationFront-rear polarityBAR domain proteinsFront-rear polarizationClathrin-independent internalizationSpecific endocytic pathwaysVEGFR2 endocytosisEndocytic pathwayAngiogenesis defectsEffector PAKTip cellsSlit-RoboActivation of VEGFR2Downstream activationVEGFR2 internalizationCell behaviorPathological angiogenesisCritical mediatorEndocytosisPathological conditions
2017
Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics
Lacy MM, Baddeley D, Berro J. Single-molecule imaging of the BAR-domain protein Pil1p reveals filament-end dynamics. Molecular Biology Of The Cell 2017, 28: 2251-2259. PMID: 28659415, PMCID: PMC5555653, DOI: 10.1091/mbc.e17-04-0238.Peer-Reviewed Original ResearchConceptsBAR domain proteinsSingle-molecule strategySingle-molecule imagingOligomeric filamentsFission yeastConventional fluorescence microscopyDynamic filamentsCytoplasmic faceMolecular assembliesLocalized recruitmentPlasma membraneEisosomesMacromolecular assembliesProtein dynamicsStable assemblyFluorescence microscopySuch assembliesPhysiological conditionsNanometer-scale featuresFirst direct observationProteinAssemblyHeterogeneous dynamicsPhotobleachingFilaments
2009
Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits
Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Ferguson A, Destaing O, Ko G, Takasaki J, Cremona O, Toole E, De Camilli P. Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits. Developmental Cell 2009, 17: 811-822. PMID: 20059951, PMCID: PMC2861561, DOI: 10.1016/j.devcel.2009.11.005.Peer-Reviewed Original ResearchConceptsEndocytic roleEndocytic clathrin-coated pitsBAR domain proteinsSense membrane curvatureClathrin-coated pitsWild-type cellsGTPase dynaminEndocytic intermediatesHigher eukaryotesDomain proteinsMembrane fissionActin dynamicsNumerous proteinsDynaminMembrane curvatureProtein upstreamTubular neckConcerted actionProteinActinCoordinated actionKey playersFunctional relationshipCellsSequence of eventsMechanistic Insights into Membrane Remodeling Through BAR‐Domain Proteins
Frost A, Mim C, Perera R, Spasov K, Egelman E, De Camilli P, Unger V. Mechanistic Insights into Membrane Remodeling Through BAR‐Domain Proteins. The FASEB Journal 2009, 23: 82.2-82.2. DOI: 10.1096/fasebj.23.1_supplement.82.2.Peer-Reviewed Original ResearchF-BAR domainBAR domain proteinsMembrane-bound stateDifferent molecular surfacesMembrane remodelingCellular physiologyMembrane curvatureElectron cryomicroscopyMechanistic insightsCooperative assemblyConstituent membranesProteinStructural informationMolecular surfaceFunctional stateMembraneDomainSubtle changesCryomicroscopyBilayer substratePhysiologyStructural modelAssemblyRemodelingCells
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