Jaime Grutzendler MD
Associate Professor of Neurology and of Neurobiology; Director, Yale Center for Experimental Neuroimaging (YCEN)
Neuro-glio-vascular interactions, cerebral blood flow and metabolism blood-brain barrier, two-photon microscopy, myelin imaging, synaptic plasticity, development and pathology of the brain microvasculature, Neuroinflammationin, neurodegenerative diseases and stroke
The dynamics and logic of cell-cell interactions in the neurovascular unit
The overall interest of our laboratory centers around cells in the so called "neuro-glio-vascular unit" (neurons, endothelium, astrocytes, pericytes and microglia). Our goal is to learn about the dynamic properties of these cells and how cell-cell interactions are disrupted in brain injury, vascular and neurodegenerative pathologies. The majority of projects in the laboratory are imaging-oriented. We use a variety of techniques including transcranial in vivo two photon microscopy, high-resolution confocal, spectral confocal reflectance microscopy (SCoRe) and electron microscopy in combination with differential fluorescent cell labeling and genetic/pharmacological manipulations.
Extensive Research Description
- IN VIVO IMAGING: Our lab is interested in developeing novel imaging strategies and methods to better understand the pathophysiology of a variety of neurological disorders. We aim to investigate in vivo the normal and pathological cellular dynamics of a variety of cell types including neurons (synapses), microglia, astrocytes,microvessels and oligodendrocytes.
- NEURODEGENERATION: understanding the dynamics of neurodegenerative conditions such as Alzheimer's disease. We study the interactions between microglia, neurons and amyloid plaques to understand the sequence of events leading to neural dysfunction in AD.
- MICROVASCULAR PLASTICITY: microvascular recanalization and angioplasticity after cerebral embolic occlusion. We have recently described a new mechanism of microvascular plasticity which leads to vessel recanalization (Angiophagy). We are investigating the molecular control and physiological relevance of this novel mechanism.
- "NEUROVASCULAR UNIT" FUNCTION AND PATHOLOGY: neurovascular coupling, tissue oxygenation. Development and disruption of the neurovascular unit. Understanding how neurons and astrocytes adapt to metabolic challenges.
- AXON AND SYNAPSE DEGENERATION/REGENERATION: Wa have recently developed a method for imaging myelinated axons in vivo (Spectral confocal reflectance microscopy SCoRe), which we are currently using to explore questions related to myelin's role in regeneration and degeneration.
- NEURO-IMMUNE FUNCTION: interactions between the immune and nervous systems in the normal and pathological brain.