2025
Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope
Chandler T, Guo M, Su Y, Chen J, Wu Y, Liu J, Agashe A, Fischer R, Mehta S, Kumar A, Baskin T, Jaumouillé V, Liu H, Swaminathan V, Nain A, Oldenbourg R, La Riviere P, Shroff H. Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope. Proceedings Of The National Academy Of Sciences Of The United States Of America 2025, 122: e2406679122. PMID: 39982748, DOI: 10.1073/pnas.2406679122.Peer-Reviewed Original ResearchConceptsPhalloidin-labeled actinXylem cellsPolarized fluorescence microscopyGiant unilamellar vesiclesU2OS cellsActin alignmentOrientation distribution functionCellular coordinationEfficiency of excitationMouse fibroblastsModel of image formationPolarization-dependent efficiencyActinFluorescence microscopyPhysics-informed modelThree-dimensional orientationFluorescent dipolesLight-sheet microscopeUnilamellar vesiclesCoarse-grained representationThree-dimensional distributionDistribution functionCellular structureProfiling toolsXylem
2022
The evolution of performing a kidney biopsy: a single center experience comparing native and transplant kidney biopsies performed by interventional radiologists and nephrologists
Emelianova D, Prikis M, Morris CS, Gibson PC, Solomon R, Scriver G, Smith ZT, Bhave A, Shields J, DeSarno M, Kumar A. The evolution of performing a kidney biopsy: a single center experience comparing native and transplant kidney biopsies performed by interventional radiologists and nephrologists. BMC Nephrology 2022, 23: 226. PMID: 35752759, PMCID: PMC9233823, DOI: 10.1186/s12882-022-02860-1.Peer-Reviewed Original ResearchConceptsKidney biopsyComplication rateNative biopsiesInterventional radiologistsCovariates of interestBiopsy procedureSingle-center retrospective analysisSignificant differencesCenter retrospective analysisSingle-center experienceMultivariable logistic regressionTransplant kidney biopsiesLikelihood of complicationsReal-time ultrasoundHemoglobin checkBaseline characteristicsCenter experienceFrequent complicationMicroscopic hematuriaPost biopsyBlood pressureEntire cohortGeneral anesthesiaBlood countKidney disease