2004
The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity
Xu J, Wang P, Li Y, Li G, Kaczmarek LK, Wu Y, Koni PA, Flavell RA, Desir GV. The voltage-gated potassium channel Kv1.3 regulates peripheral insulin sensitivity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 3112-3117. PMID: 14981264, PMCID: PMC365752, DOI: 10.1073/pnas.0308450100.Peer-Reviewed Original ResearchMeSH KeywordsAdipose TissueAnimalsBiological TransportFastingGlucoseInsulinInterleukin-6JNK Mitogen-Activated Protein KinasesKineticsKv1.3 Potassium ChannelMaleMiceMice, Inbred C57BLMice, KnockoutMice, ObeseMitogen-Activated Protein KinasesModels, BiologicalMuscle, SkeletalPotassium ChannelsPotassium Channels, Voltage-GatedTumor Necrosis Factor-alphaConceptsKv1.3-/- micePeripheral glucose homeostasisPeripheral insulin sensitivityPlasma membraneGene inactivationInsulin sensitivityAmount of GLUT4Skeletal muscleTerminal kinase (JNK) activityGlucose homeostasisAdipose tissueLower blood insulin levelsVoltage-gated potassium channelsInsulin-stimulated glucose uptakeVoltage-gated potassium channel Kv1.3Tumor necrosis factor productionExperimental autoimmune encephalitisBlood insulin levelsHigh-fat dietPotassium channel Kv1.3Tumor necrosis factor secretionPeripheral T lymphocytesKinase activityNecrosis factor productionNumber of tissuesKv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics
Fadool DA, Tucker K, Perkins R, Fasciani G, Thompson RN, Parsons AD, Overton JM, Koni PA, Flavell RA, Kaczmarek LK. Kv1.3 Channel Gene-Targeted Deletion Produces “Super-Smeller Mice” with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics. Neuron 2004, 41: 389-404. PMID: 14766178, PMCID: PMC2737549, DOI: 10.1016/s0896-6273(03)00844-4.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAdaptor Proteins, Vesicular TransportAnimalsBehavior, AnimalBlotting, WesternBody WeightBrain-Derived Neurotrophic FactorCalcium ChannelsCells, CulturedDensitometryDifferential ThresholdDiscrimination, PsychologicalDose-Response Relationship, DrugDrinkingElectric StimulationEmbryo, MammalianEnergy IntakeExploratory BehaviorGene DeletionGRB10 Adaptor ProteinHabituation, PsychophysiologicHumansInsulinKidneyKineticsKv1.3 Potassium ChannelMembrane PotentialsMiceMice, KnockoutMotor ActivityNerve Tissue ProteinsNeuronsNeurotoxinsNuclear Matrix-Associated ProteinsOdorantsOlfactory BulbPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Voltage-GatedProteinsRas ProteinsReceptor, trkBReverse Transcriptase Polymerase Chain ReactionRNA, MessengerScorpion VenomsSensory ThresholdsSrc-Family KinasesTime FactorsTyrosine 3-MonooxygenaseConceptsKv1.3-/- miceProtein-protein interactionsGene-targeted deletionKv1.3-null miceSignal transductionScaffolding proteinSignaling cascadesChannel genesC-type inactivationDeletionMembrane potentialNull miceOlfactory codingDetection of odorsPotassium channelsKv1.3 channelsProteinSense of smellSlow inactivation kineticsWild-type miceTransductionGenesOlfactory bulb mitral cellsMiceRole
1997
Regulation by insulin of a unique neuronal Ca2+ pool and of neuropeptide secretion
Jonas E, Knox R, Smith T, Wayne N, Connor J, Kaczmarek L. Regulation by insulin of a unique neuronal Ca2+ pool and of neuropeptide secretion. Nature 1997, 385: 343-346. PMID: 9002519, DOI: 10.1038/385343a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoquinonesCalciumCalcium ChannelsCells, CulturedCyclic AMPEndoplasmic ReticulumHeparinInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsInsulinInvertebrate HormonesLactams, MacrocyclicNeuronsNeuropeptidesProtein-Tyrosine KinasesQuinonesReceptors, Cytoplasmic and NuclearRifabutinThapsigarginConceptsIntracellular Ca2Neuropeptide secretionSpontaneous action potentialsEffect of insulinSecretion of neuropeptidesTyrosine kinase receptorsAcute riseBag cell neuronsDistal tipNeuronal dischargeNeuronal Ca2Distinct intracellular poolsCell neuronsAction potentialsCyclic AMP analogueInsulinNeuropeptidesInsulin receptorKinase receptorsSecretionPresumed siteNeuronsIntracellular poolMitochondrial Ca2Receptors
1996
Insulin receptor in Aplysia neurons: characterization, molecular cloning, and modulation of ion currents
Jonas E, Knox R, Kaczmarek L, Schwartz J, Solomon D. Insulin receptor in Aplysia neurons: characterization, molecular cloning, and modulation of ion currents. Journal Of Neuroscience 1996, 16: 1645-1658. PMID: 8774433, PMCID: PMC6578688, DOI: 10.1523/jneurosci.16-05-01645.1996.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino Acid SequenceAnimalsAplysiaBase SequenceCalcium ChannelsCloning, MolecularDNA, ComplementaryElectrophysiologyImmunohistochemistryInsulinIon ChannelsMolecular ProbesMolecular Sequence DataNeuronsPotassium ChannelsProtein-Tyrosine KinasesReceptor, InsulinTissue DistributionConceptsBag cell neuronsInsulin receptorInsulin-like peptidesImmunocytochemical staining showCell neuronsTyrosine kinase receptorsVertebrate insulinsMolecular cloningHerbimycin ATyrosine residuesTyrosine kinaseKinase receptorsInsulin-like growth factor-1Factor 1Staining showsVoltage-clamped neuronsVoltage-dependent Ca2Growth factor-1Aplysia californicaAplysia neuronsNervous systemReceptorsAction potentialsNeuronsInsulin