2022
LINE-1 activation in the cerebellum drives ataxia
Takahashi T, Stoiljkovic M, Song E, Gao XB, Yasumoto Y, Kudo E, Carvalho F, Kong Y, Park A, Shanabrough M, Szigeti-Buck K, Liu ZW, Kristant A, Zhang Y, Sulkowski P, Glazer PM, Kaczmarek LK, Horvath TL, Iwasaki A. LINE-1 activation in the cerebellum drives ataxia. Neuron 2022, 110: 3278-3287.e8. PMID: 36070749, PMCID: PMC9588660, DOI: 10.1016/j.neuron.2022.08.011.Peer-Reviewed Original ResearchConceptsLINE-1 activationL1 activationAtaxia telangiectasia patientsNuclear element-1Transposable elementsEpigenetic silencersHuman genomeL1 promoterMolecular regulatorsDNA damagePurkinje cell dysfunctionElement 1First direct evidenceTelangiectasia patientsDirect targetingCerebellar expressionNeurodegenerative diseasesDisease etiologyCalcium homeostasis
2016
International Union of Basic and Clinical Pharmacology. C. Nomenclature and Properties of Calcium-Activated and Sodium-Activated Potassium Channels
Kaczmarek LK, Aldrich RW, Chandy KG, Grissmer S, Wei AD, Wulff H. International Union of Basic and Clinical Pharmacology. C. Nomenclature and Properties of Calcium-Activated and Sodium-Activated Potassium Channels. Pharmacological Reviews 2016, 69: 1-11. PMID: 28267675, PMCID: PMC11060434, DOI: 10.1124/pr.116.012864.Peer-Reviewed Original ResearchT Cell Receptor Mediated Calcium Entry Requires Alternatively Spliced Cav1.1 Channels
Matza D, Badou A, Klemic KG, Stein J, Govindarajulu U, Nadler MJ, Kinet JP, Peled A, Shapira OM, Kaczmarek LK, Flavell RA. T Cell Receptor Mediated Calcium Entry Requires Alternatively Spliced Cav1.1 Channels. PLOS ONE 2016, 11: e0147379. PMID: 26815481, PMCID: PMC4729531, DOI: 10.1371/journal.pone.0147379.Peer-Reviewed Original Research
2010
Controlling auditory excitability: the benefits of a cultured environment
Kaczmarek LK. Controlling auditory excitability: the benefits of a cultured environment. The Journal Of Physiology 2010, 588: 1387-1388. PMID: 20436041, PMCID: PMC2876793, DOI: 10.1113/jphysiol.2010.189712.Peer-Reviewed Original Research
2007
Comparative effects of sodium pyrithione evoked intracellular calcium elevation in rodent and primate ventral horn motor neurons
Knox RJ, Keen KL, Luchansky L, Terasawa E, Freyer H, Barbee SJ, Kaczmarek LK. Comparative effects of sodium pyrithione evoked intracellular calcium elevation in rodent and primate ventral horn motor neurons. Biochemical And Biophysical Research Communications 2007, 366: 48-53. PMID: 18053804, DOI: 10.1016/j.bbrc.2007.11.083.Peer-Reviewed Original ResearchConceptsNeuron-specific enolaseMotor neuronsDose-response curveFura-PE3/AMVentral horn motor neuronsAplysia neuronsSpinal cord slicesIntracellular calcium elevationStore-operated calcium entryCord slicesHindlimb weaknessSpecific enolaseSKF-96365Oral administrationRhesus monkey embryosCalcium entryEffects of NAPCalcium elevationPersistent influxNeuronsMammalian neuronsIslet-1Sodium pyrithioneComparative effectsPlasma membraneSodium‐dependent potassium channels of a Slack‐like subtype contribute to the slow afterhyperpolarization in lamprey spinal neurons
Wallén P, Robertson B, Cangiano L, Löw P, Bhattacharjee A, Kaczmarek LK, Grillner S. Sodium‐dependent potassium channels of a Slack‐like subtype contribute to the slow afterhyperpolarization in lamprey spinal neurons. The Journal Of Physiology 2007, 585: 75-90. PMID: 17884929, PMCID: PMC2375474, DOI: 10.1113/jphysiol.2007.138156.Peer-Reviewed Original ResearchConceptsSodium-dependent potassium channelSlow afterhyperpolarizationAction potentialsPotassium channelsSingle action potentialLamprey spinal neuronsLamprey spinal cordLamprey locomotor networkSlow AHPLarge neuronsSpinal neuronsSpinal cordLocomotor networksBurst activityKNa channelsDistinct immunoreactivityGray matterReversal potentialNeuronsChloride injectionAfterhyperpolarizationRapid activationFunctional roleHigh-level activitiesSlack gene
2005
For K+ channels, Na+ is the new Ca2+
Bhattacharjee A, Kaczmarek LK. For K+ channels, Na+ is the new Ca2+. Trends In Neurosciences 2005, 28: 422-428. PMID: 15979166, DOI: 10.1016/j.tins.2005.06.003.Peer-Reviewed Original ResearchRequirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions
Badou A, Basavappa S, Desai R, Peng YQ, Matza D, Mehal WZ, Kaczmarek LK, Boulpaep EL, Flavell RA. Requirement of Voltage-Gated Calcium Channel ß4 Subunit for T Lymphocyte Functions. Science 2005, 307: 117-121. PMID: 15637280, DOI: 10.1126/science.1100582.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channels, L-TypeCalcium SignalingCD4-Positive T-LymphocytesCytokinesDNA-Binding ProteinsIon Channel GatingLymphocyte ActivationMembrane PotentialsMiceMice, Inbred C3HMice, Inbred C57BLMutationNFATC Transcription FactorsNuclear ProteinsPatch-Clamp TechniquesPhosphorylationProtein SubunitsReceptors, Antigen, T-CellT-LymphocytesTranscription FactorsConceptsT lymphocytesCalcium channelsVoltage-gated calcium channelsT lymphocyte functionT cell receptor stimulationCell receptor stimulationCytokine productionLymphocyte functionCalcium influxReceptor stimulationCalcium responseCalcium entryTranscription factor NFATCav1 channelsLymphocytesAlpha1 subunitCav channelsNormal functionNonexcitable cellsDisplay impairmentsExcitable cellsChannel openingMolecular identityDiverse physiological processesPhysiological processes
2004
Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia
Knox RJ, Magoski NS, Wing D, Barbee SJ, Kaczmarek LK. Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia. Developmental Neurobiology 2004, 60: 411-423. PMID: 15307146, DOI: 10.1002/neu.20029.Peer-Reviewed Original ResearchConceptsAplysia bag cell neuronsWhole-cell current-clamp recordingsBag cell neuronsPlasma membraneCurrent-clamp recordingsNeuronal physiologyCytosolic pHCytosolic freeMembrane potentialCell neuronsSodium pyrithionePresence of externalRatiometric imagingMV depolarizationClose structural analogueHill coefficientNapStructural analoguesSpecies
2002
Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons
Zhang Y, Magoski NS, Kaczmarek LK. Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons. Journal Of Neuroscience 2002, 22: 10134-10141. PMID: 12451114, PMCID: PMC6758731, DOI: 10.1523/jneurosci.22-23-10134.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCells, CulturedElectric StimulationEnzyme ActivatorsEnzyme InhibitorsLarge-Conductance Calcium-Activated Potassium ChannelsNeural InhibitionNeuronsPatch-Clamp TechniquesPhloretinPotassiumPotassium Channel BlockersPotassium Channels, Calcium-ActivatedProtein Kinase CRefractory Period, ElectrophysiologicalSphingosineTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsRefractory periodBK currentsProtein kinase COnset of afterdischargeBK channel activityApplication of phloretinBK channel activatorsProlonged refractory periodAbility of stimulationRole of Ca2Blocker paxillinePharmacological characteristicsChannel activatorIntracellular Ca2Prolonged increaseOutward currentsInhibitor of PKCAfterdischargesNeuronsAdditional stimulationProlonged activationActivator of PKCChannel activity
2001
Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica
Ohnuma K, Whim M, Fetter R, Kaczmarek L, Zucker R. Presynaptic target of Ca2+ action on neuropeptide and acetylcholine release in Aplysia californica. The Journal Of Physiology 2001, 535: 647-662. PMID: 11559764, PMCID: PMC2278817, DOI: 10.1111/j.1469-7793.2001.00647.x.Peer-Reviewed Original ResearchConceptsSensory neuronsCholinergic synapsesPeptidergic synapsesAcetylcholine releasePostsynaptic responsesAction potentialsMost synaptic contactsIntracellular calcium concentrationPeptidergic vesiclesSingle action potentialAplysia californicaDose-response curveDense-core vesiclesNeuron B2Neuron B3Cholinergic transmissionSynaptic contactsLinear dose-response curvePostsynaptic neuronsPresynaptic neuronsCholinergic releasePresynaptic targetCholinergic vesiclesReleasable poolPatch pipetteCalmodulin Regulates Assembly and Trafficking of SK4/IK1 Ca2+-activated K+ Channels*
Joiner W, Khanna R, Schlichter L, Kaczmarek L. Calmodulin Regulates Assembly and Trafficking of SK4/IK1 Ca2+-activated K+ Channels*. Journal Of Biological Chemistry 2001, 276: 37980-37985. PMID: 11495911, DOI: 10.1074/jbc.m104965200.Peer-Reviewed Original ResearchConceptsChannel assemblyC-terminusAssembly of channelsIon channelsC-terminal domainDistal C-terminal domainCo-immunoprecipitation experimentsCaM-binding domainSurface expressionDominant negative effectProximal C-terminusWhole-cell currentsCellular functionsCaM genesPlasma membraneCaM proteinFree CaMNovel mechanismTraffickingProteinTerminusAssemblyExpressionSK4Domain
2000
Mitochondrial Memory Banks
Kaczmarek L. Mitochondrial Memory Banks. The Journal Of General Physiology 2000, 115: 347-350. PMID: 10694262, PMCID: PMC2217212, DOI: 10.1085/jgp.115.3.347.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsExocytotic Insertion of Calcium Channels Constrains Compensatory Endocytosis to Sites of Exocytosis
Smith R, Baibakov B, Ikebuchi Y, White B, Lambert N, Kaczmarek L, Vogel S. Exocytotic Insertion of Calcium Channels Constrains Compensatory Endocytosis to Sites of Exocytosis. Journal Of Cell Biology 2000, 148: 755-768. PMID: 10684256, PMCID: PMC2169375, DOI: 10.1083/jcb.148.4.755.Peer-Reviewed Original ResearchActivation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones
Magoski N, Knox R, Kaczmarek L. Activation of a Ca2+‐permeable cation channel produces a prolonged attenuation of intracellular Ca2+ release in Aplysia bag cell neurones. The Journal Of Physiology 2000, 522: 271-283. PMID: 10639103, PMCID: PMC2269759, DOI: 10.1111/j.1469-7793.2000.t01-2-00271.x.Peer-Reviewed Original ResearchConceptsVoltage-gated Ca2Non-selective cation channelsRefractory periodCation channelsElectrical stimulationFree salineAbility of nifedipinePresence of nifedipinePresence of TTXIntact abdominal gangliaProlonged refractory periodOnset of refractorinessAfferent inputBrief synaptic stimulationHigh external potassiumPermeable cation channelIntracellular calciumAbdominal ganglionExtracellular Ca2Synaptic stimulationAfterdischargesNormal responseIntracellular Ca2Intracellular storesRepeated stimulation
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1998
Heterologous Expression of the Kv3.1 Potassium Channel Eliminates Spike Broadening and the Induction of a Depolarizing Afterpotential in the Peptidergic Bag Cell Neurons
Whim M, Kaczmarek L. Heterologous Expression of the Kv3.1 Potassium Channel Eliminates Spike Broadening and the Induction of a Depolarizing Afterpotential in the Peptidergic Bag Cell Neurons. Journal Of Neuroscience 1998, 18: 9171-9180. PMID: 9801357, PMCID: PMC6792887, DOI: 10.1523/jneurosci.18-22-09171.1998.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsAction potentialsCalcium entryUse-dependent inactivationExpression of Kv3.1Kv3.1 potassium channelPeptidergic bag cell neuronsControl neuronsSpontaneous firingBrief synaptic stimulationCalcium currentNeuronal excitabilityIntracellular calciumCalcium influxSynaptic stimulationDepolarizing afterpotentialsBAPTA-AMAfterpotentialsNeuronsPotassium channelsClusters of cellsKv3.1InductionPhysiological roleDepolarization Selectively Increases the Expression of the Kv3.1 Potassium Channel in Developing Inferior Colliculus Neurons
Liu S, Kaczmarek L. Depolarization Selectively Increases the Expression of the Kv3.1 Potassium Channel in Developing Inferior Colliculus Neurons. Journal Of Neuroscience 1998, 18: 8758-8769. PMID: 9786983, PMCID: PMC6793528, DOI: 10.1523/jneurosci.18-21-08758.1998.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAnimals, NewbornCalciumGene Expression Regulation, DevelopmentalIn Vitro TechniquesInferior ColliculiMembrane PotentialsNeuropeptidesPatch-Clamp TechniquesPotassiumPotassium ChannelsPotassium Channels, Voltage-GatedRatsRats, Sprague-DawleyRNA, MessengerShaw Potassium ChannelsConceptsInferior colliculus neuronsOnset of hearingColliculus neuronsCalcium influxChannel subunitsPotassium currentAction potentialsElevated external potassium concentrationCalcium channel blockersDepolarization-induced increaseSpontaneous neuronal activityNoninactivating potassium currentKv3.1 potassium channelVoltage-clamp experimentsChannel blockersNeuronal excitabilityElevated potassiumAuditory neuronsNeuronal activityExternal potassium concentrationExternal potassium ionsNeuronsPotassium channelsMRNA levelsMarked increaseHigh-frequency firing helps replenish the readily releasable pool of synaptic vesicles
Wang L, Kaczmarek L. High-frequency firing helps replenish the readily releasable pool of synaptic vesicles. Nature 1998, 394: 384-388. PMID: 9690475, DOI: 10.1038/28645.Peer-Reviewed Original ResearchConceptsReleasable poolPotassium channel blocker tetraethylammoniumChannel blocker Cd2Synaptic vesiclesPresynaptic action potentialHigh-frequency stimulationVoltage-gated Ca2Short-term synaptic depressionCentral nervous systemPatch-clamp recordingsHigh-frequency firingGiant synapsesPostsynaptic mechanismsBuffer EGTAMouse auditoryBlocker tetraethylammoniumSynaptic activitySynaptic depressionPresynaptic terminalsNervous systemAction potentialsRate of replenishmentSynapsesCa2Key signal
1997
hSK4, a member of a novel subfamily of calcium-activated potassium channels
Joiner W, Wang L, Tang M, Kaczmarek L. hSK4, a member of a novel subfamily of calcium-activated potassium channels. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 11013-11018. PMID: 9380751, PMCID: PMC23566, DOI: 10.1073/pnas.94.20.11013.Peer-Reviewed Original ResearchConceptsCalcium-activated potassium channelsSK channelsSmall-conductance calcium-activated potassium channelsPotassium channelsHSK4Novel subfamilyAdult animalsPredominant expressionLeucine zipper-like domainChinese hamster ovary cellsNonexcitable tissuesHamster ovary cellsChannel polypeptideOvary cellsLow homologyC-terminusHigh affinityUnknown function