2023
Effects of Ethanolic and Aqueous Extracts of Garcinia gardneriana Leaves in an In Vivo Experimental Model Induced by a Hyperlipidic Diet
do Espirito Santo B, Santana L, Kato W, de Araújo F, Tatara M, Croda J, Bogo D, de Cássia Freitas K, de Cássia Avellaneda Guimarães R, Hiane P, Pott A, de Oliveira Filiú W, de Faria B, de Oliveira Figueiredo P, do Nascimento V, Ayres F, de Oliveira Bastos P. Effects of Ethanolic and Aqueous Extracts of Garcinia gardneriana Leaves in an In Vivo Experimental Model Induced by a Hyperlipidic Diet. Nutrients 2023, 15: 1308. PMID: 36986038, PMCID: PMC10051817, DOI: 10.3390/nu15061308.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiet, High-FatEthanolFatty LiverGarciniaMicePlant ExtractsPlants, MedicinalWaterConceptsMonocyte chemoattractant protein-1 concentrationsChemoattractant protein-1 concentrationsNon-communicable chronic diseasesExperimental modelProtein-1 concentrationsHigh-fat dietHigh-density lipoproteinPossible metabolic alterationsVivo experimental modelsEffect of ethanolicAqueous extractTotal cholesterolInterleukin-10Microvesicular steatosisHepatic steatosisInsulin resistanceBlood glucoseSwiss miceHyperlipidic dietChronic diseasesDensity lipoproteinMetabolic alterationsControl groupWeight gainEthanolic extract
2022
Effects of the Seed Oil of Carica papaya Linn on Food Consumption, Adiposity, Metabolic and Inflammatory Profile of Mice Using Hyperlipidic Diet
Santana L, do Espirito Santo B, Tatara M, Negrão F, Croda J, Alves F, de Oliveira Filiú W, Cavalheiro L, Nazário C, Asato M, de Faria B, do Nascimento V, de Cássia Avellaneda Guimarães R, de Cássia Freitas K, Hiane P. Effects of the Seed Oil of Carica papaya Linn on Food Consumption, Adiposity, Metabolic and Inflammatory Profile of Mice Using Hyperlipidic Diet. Molecules 2022, 27: 6705. PMID: 36235241, PMCID: PMC9570947, DOI: 10.3390/molecules27196705.Peer-Reviewed Original ResearchConceptsOral glucose toleranceHigh-fat dietArea of adipocytesInsulin sensitivity testAcute toxicity testInflammatory profileGlucose toleranceTotal cholesterolFatty acidsIL-6Insulin resistanceMCP-1Swiss miceHyperlipidic dietLower insulinCaloric intakeMonounsaturated fatty acidsProtective effectHypoglycemic effectAdipose tissueCytokine resistanceWeight gainAdiposityFood consumptionFatty acid composition
2021
High-Fat Diet with Lyophilized Acrocomia aculeata Pulp Increases High-Density Lipoprotein-Cholesterol Levels and Inhibits Adipocyte Hypertrophy in Mice
Fernandes M, Rezende K, Inada A, de Cássia Freitas K, de Oliveira Filiú W, Cavalheiro L, Nazário C, Hiane P, Croda J, Tatara M, de Souza A, Pott A, de Cássia Avellaneda Guimarães R. High-Fat Diet with Lyophilized Acrocomia aculeata Pulp Increases High-Density Lipoprotein-Cholesterol Levels and Inhibits Adipocyte Hypertrophy in Mice. Journal Of Medicinal Food 2021, 24: 841-851. PMID: 34342510, DOI: 10.1089/jmf.2020.0124.Peer-Reviewed Original ResearchConceptsDensity lipoprotein cholesterol levelsLipoprotein cholesterol levelsHigh-fat dietChronic noncommunicable diseasesHF diet groupSevere comorbiditiesFatty acidsCholesterol HDLAdipocyte hypertrophyFat dietSwiss miceHF dietRelevant health hazardWestern dietDiet groupHistological parametersProtective effectNoncommunicable diseasesBiochemical parametersBeneficial effectsFat depositsMiceBrazilian palmDietHealth parametersAnti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves
Santos E, Silva-Filho S, Santos Radai J, Arena A, Fraga T, Lima Cardoso C, Croda J, Leite Kassuya C. Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves. Journal Of Ethnopharmacology 2021, 278: 114308. PMID: 34102271, DOI: 10.1016/j.jep.2021.114308.Peer-Reviewed Original ResearchConceptsAnti-inflammatory propertiesMethylthiazolyldiphenyl-tetrazolium bromideComplete Freund's adjuvantAnti-inflammatory featuresEthanolic extractLong-term treatmentBacillus Calmette-GuerinInflammatory evaluationInflammatory parametersConventional medicationsDaily administrationNeutrophil activityPersistent inflammationRheumatic diseasesFreund's adjuvantInflammatory aspectsPleurisy modelTraditional folk medicineSwiss miceCalmette-GuerinInflammatory diseasesC57BL6 miceVivo modelExperimental modelAdverse effects
2020
Caryocar brasiliense Cambess. Pulp Oil Supplementation Reduces Total Cholesterol, LDL-c, and Non-HDL-c in Animals
Silva G, Di Pietro Fernandes C, Hiane P, de Cássia Freitas K, Figueiredo P, Inada A, Filiú W, Maldonade I, Nunes Â, de Oliveira L, Caires A, Michels F, Candido C, Cavalheiro L, Asato M, Donadon J, de Faria B, Tatara M, Croda J, Pott A, Nazário C, de Cássia Avellaneda Guimarães R. Caryocar brasiliense Cambess. Pulp Oil Supplementation Reduces Total Cholesterol, LDL-c, and Non-HDL-c in Animals. Molecules 2020, 25: 4530. PMID: 33022905, PMCID: PMC7582708, DOI: 10.3390/molecules25194530.Peer-Reviewed Original ResearchAntimicrobial activity of plant extracts against carbapenem-producing Klebsiella pneumoniae and in vivo toxicological assessment
Vasconcelos N, Vaz M, Radai J, Kassuya C, Formagio A, Graciani F, Leal M, Oliveira R, da Silva K, Croda J, Simionatto S. Antimicrobial activity of plant extracts against carbapenem-producing Klebsiella pneumoniae and in vivo toxicological assessment. Journal Of Toxicology And Environmental Health Part A 2020, 83: 719-729. PMID: 32981476, DOI: 10.1080/15287394.2020.1824135.Peer-Reviewed Original ResearchConceptsSwiss male miceMultidrug-resistant strainsSplenic phagocytosisMale miceAgar disk diffusionBeneficial effectsToxicological assessmentMicronucleus assayKlebsiella pneumoniaePlant extractsVivo toxicological assessmentDisk diffusionAntibioticsAntimicrobial activityMutagenic activityGlobal spreadExtractMolecular characterizationAssessmentAntibacterial activityActivityMiceActive productsSynergismBlutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in mice
Kassuya R, Radai J, Macorini L, Nunes V, Salvador M, Leite P, Oliveira R, Croda J, Arena A, Kassuya C. Blutaparon portulacoides ethanolic extract reduced IL-1β and inflammatory parameters induced by the Mycobacterium complex and carrageenan in mice. Inflammopharmacology 2020, 29: 439-450. PMID: 32910315, DOI: 10.1007/s10787-020-00752-0.Peer-Reviewed Original ResearchMeSH KeywordsAmaranthaceaeAnimalsAnti-Inflammatory AgentsAntitubercular AgentsCarrageenanDisease Models, AnimalDose-Response Relationship, DrugEdemaFemaleInflammationInterleukin-1betaMaleMiceMice, Inbred C57BLMicrobial Sensitivity TestsMycobacterium tuberculosisPlant ExtractsPleurisyRatsRats, WistarConceptsSwiss miceIL-1β levelsC57BL-6 miceEthanolic extractInflammation/infectionSafe new drugsM. tuberculosis growthMechanical hyperalgesiaInflammatory parametersOral treatmentIL-1βPleurisy modelMinimum inhibitory concentrationPharmacoeconomic perspectiveC57BL6 miceComplete adjuvantSubcutaneous injectionOral administrationWistar ratsMiceMycobacterium tuberculosisNew drugsHealth benefitsTuberculosis growthInhibitory concentrationYellow fever transmission in non-human primates, Bahia, Northeastern Brazil
de Jesus J, Gräf T, Giovanetti M, Mares-Guia M, Xavier J, Maia M, Fonseca V, Fabri A, dos Santos R, Pereira F, Santos L, da Silva L, Maia Z, Cerqueira J, Thèze J, Abade L, de Carvalho Santana Cordeiro M, Torquato S, Santana E, de Jesus Silva N, Dourado R, Alves A, do Socorro Guedes A, da Silva Filho P, Faria N, de Albuquerque C, de Abreu A, Romano A, Croda J, do Carmo Said R, Cunha G, da Fonseca Cerqueira J, de Mello A, de Filippis A, Alcantara L. Yellow fever transmission in non-human primates, Bahia, Northeastern Brazil. PLOS Neglected Tropical Diseases 2020, 14: e0008405. PMID: 32780745, PMCID: PMC7418952, DOI: 10.1371/journal.pntd.0008405.Peer-Reviewed Original ResearchConceptsYellow fever virusNon-human primatesHuman casesPositive tissue samplesYFV genomesAutochthonous human casesClinical syndromeMolecular epidemiologySurveillance teamYFV transmissionFever virusTissue samplesYellow fever transmissionYFV outbreakGenotype IPrimatesAffected areaHepatitisHumansCasesSyndromeEpidemiologyEffect of Supplementation with Hydroethanolic Extract of Campomanesia xanthocarpa (Berg.) Leaves and Two Isolated Substances from the Extract on Metabolic Parameters of Mice Fed a High-Fat Diet
Cardozo C, Inada A, Cardoso C, de Oliveira Filiú W, de Farias B, Alves F, Tatara M, Croda J, de Cássia Avellaneda Guimarães R, Hiane P, de Cássia Freitas K. Effect of Supplementation with Hydroethanolic Extract of Campomanesia xanthocarpa (Berg.) Leaves and Two Isolated Substances from the Extract on Metabolic Parameters of Mice Fed a High-Fat Diet. Molecules 2020, 25: 2693. PMID: 32531999, PMCID: PMC7321075, DOI: 10.3390/molecules25112693.Peer-Reviewed Original ResearchConceptsHigh-fat dietInterleukin-6Diet-induced obesity modelBeneficial effectsHydroethanolic extractMale Swiss miceHydroethanolic leaf extractEffect of supplementationLipid parametersMice fedAdiposity gainFat dietSwiss miceObesity modelObesity featuresMetabolic parametersFood intakeHistological parametersMetabolic disordersBody weightNutrition dietProtein expressionDietFood behaviorFurther investigation
2019
Return of the founder Chikungunya virus to its place of introduction into Brazil is revealed by genomic characterization of exanthematic disease cases
Maia Z, Pereira F, do Carmo Said R, Fonseca V, Gräf T, de Bruycker Nogueira F, Nardy V, Xavier J, Maia M, Abreu A, de Albuquerque C, Oliveira W, Croda J, de Filippis A, Cunha R, Lourenço J, de Oliveira T, Faria N, Alcantara L, Giovanetti M. Return of the founder Chikungunya virus to its place of introduction into Brazil is revealed by genomic characterization of exanthematic disease cases. Emerging Microbes & Infections 2019, 9: 53-57. PMID: 31880218, PMCID: PMC6968431, DOI: 10.1080/22221751.2019.1701954.Peer-Reviewed Original ResearchConceptsNumber of patientsGenomic surveillance strategiesFebrile illnessArboviral infectionsEpidemic seasonChikungunya virusWhole-genome sequencingDisease casesSurveillance strategiesGenetic diversityEpidemiological analysisMolecular clockCHIKV ECSA genotypeTransmission potentialGenome sequencingArboviral transmissionGenomic characterizationCHIKVLocal persistenceDetection, survival and infectious potential of Mycobacterium tuberculosis in the environment: a review of the evidence and epidemiological implications
Martinez L, Verma R, Croda J, Horsburgh C, Walter K, Degner N, Middelkoop K, Koch A, Hermans S, Warner D, Wood R, Cobelens F, Andrews J. Detection, survival and infectious potential of Mycobacterium tuberculosis in the environment: a review of the evidence and epidemiological implications. European Respiratory Journal 2019, 53: 1802302. PMID: 31048345, PMCID: PMC6753378, DOI: 10.1183/13993003.02302-2018.Peer-Reviewed Original ResearchConceptsInfectious tuberculosis patientsTuberculosis patientsTB transmissionCongregate settingsMouse modelGuinea pigsInfectious threatsMycobacterium tuberculosisDroplet nucleiConsistent findingScientific evidenceInfectious potentialEpidemiological implicationsPrimary routeFurther investigationSeminal experimental studiesRecent studiesHuman sourcesEvidencePatientsTuberculosisFindingsStudyInfectiousnessViability
2018
Building capacity for advances in tuberculosis research; proceedings of the third RePORT international meeting
van der Heijden Y, Abdullah F, Andrade B, Andrews J, Christopher D, Croda J, Ewing H, Haas D, Hatherill M, Horsburgh C, Mave V, Nakaya H, Rolla V, Srinivasan S, Sugiyono R, Ugarte-Gil C, Hamilton C. Building capacity for advances in tuberculosis research; proceedings of the third RePORT international meeting. Tuberculosis 2018, 113: 153-162. PMID: 30514497, PMCID: PMC6349374, DOI: 10.1016/j.tube.2018.09.009.Peer-Reviewed Original ResearchConceptsType 2 diabetes mellitusTuberculosis researchActive pulmonary tuberculosisHigh-risk populationEnrollment of participantsContext of HIVUnderstanding of tuberculosisHousehold contactsActive tuberculosisObservational cohortPulmonary tuberculosisDiabetes mellitusTuberculosis transmissionClinical dataIncipient tuberculosisNovel biomarkersTB participantsHost immunityStandardized research protocolsTuberculosisResearch capacity developmentHIVResearch protocolBiomarker identificationInternational meetingsThe safe use of Doliocarpus dentatus in the gestational period: Absence of changes in maternal reproductive performance, embryo-fetal development and DNA integrity
Ishikawa R, Vani J, das Neves S, Rabacow A, Kassuya C, Croda J, Cardoso C, Monreal A, Antoniolli A, Cunha-Laura A, Oliveira R. The safe use of Doliocarpus dentatus in the gestational period: Absence of changes in maternal reproductive performance, embryo-fetal development and DNA integrity. Journal Of Ethnopharmacology 2018, 217: 1-6. PMID: 29408581, DOI: 10.1016/j.jep.2018.01.034.Peer-Reviewed Original ResearchConceptsFemale Swiss miceDoliocarpus dentatusSwiss miceEmbryofetal developmentGestational periodControl groupPregnant female Swiss miceEnd of pregnancyEmbryo-fetal developmentMaternal reproductive performanceReproductive performanceSafe useEmbryofoetal developmentUrinary retentionOral gavageAbsence of changesDNA integrityAntimycobacterial actionFetal developmentReproductive parametersFormation of micronucleiEthanolic extractPregnancySignificant differencesExperimental group
2017
Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol
do Nascimento K, Moreira F, Santos J, Kassuya C, Croda J, Cardoso C, do Carmo Vieira M, Ruiz A, Foglio M, de Carvalho J, Formagio A. Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of Psidium guineense Sw. and spathulenol. Journal Of Ethnopharmacology 2017, 210: 351-358. PMID: 28844678, DOI: 10.1016/j.jep.2017.08.030.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Inflammatory AgentsAntineoplastic Agents, PhytogenicAntioxidantsAntitubercular AgentsCell Line, TumorCell ProliferationDisease Models, AnimalEdemaFemaleGas Chromatography-Mass SpectrometryHumansInhibitory Concentration 50MaleMiceOils, VolatilePlant ExtractsPlant LeavesPleurisyPsidiumRatsRats, WistarSesquiterpenesConceptsGas chromatography-mass spectrometryNuclear magnetic resonanceAntimycobacterial activityModerate antimycobacterial activityChromatography-mass spectrometryAnti-inflammatory effectsOvarian cancer cell linesAnti-inflammatory potentialAnti-Mycobacterium tuberculosis activityPsidium guineense SwEssential oilCancer cell linesSulforhodamine B assayChromatographic columnPG-1Pleurisy modelInflammatory diseasesOral administrationAntioxidant activityAntimycobacterial propertiesREMA methodTuberculosis activityB assaySignificant inhibitionCell proliferationAnti-inflammatory, antimycobacterial and genotoxic evaluation of Doliocarpus dentatus
Ishikawa R, Leitão M, Kassuya R, Macorini L, Moreira F, Cardoso C, Coelho R, Pott A, Gelfuso G, Croda J, Oliveira R, Kassuya C. Anti-inflammatory, antimycobacterial and genotoxic evaluation of Doliocarpus dentatus. Journal Of Ethnopharmacology 2017, 204: 18-25. PMID: 28392458, DOI: 10.1016/j.jep.2017.04.004.Peer-Reviewed Original ResearchConceptsMinimal inhibitory concentrationDoliocarpus dentatusPeripheral leukocyte countAnti-inflammatory agentsMechanical hypersensitivityUrine retentionCarrageenan modelLeukocyte countOral administrationLeukocyte migrationInflammation processPhagocytic activityPhagocytosis testMycobacterium tuberculosisMicronucleus testSignificant decreaseEthanolic extractGenotoxic aspectsGenotoxic evaluationInhibitory concentrationAntimycobacterial activitySafe useBetulinic acidMagnetic resonanceCount
2016
Limonene reduces hyperalgesia induced by gp120 and cytokines by modulation of IL-1 β and protein expression in spinal cord of mice
Piccinelli A, Morato P, dos Santos Barbosa M, Croda J, Sampson J, Kong X, Konkiewitz E, Ziff E, Amaya-Farfan J, Kassuya C. Limonene reduces hyperalgesia induced by gp120 and cytokines by modulation of IL-1 β and protein expression in spinal cord of mice. Life Sciences 2016, 174: 28-34. PMID: 27888114, DOI: 10.1016/j.lfs.2016.11.017.Peer-Reviewed Original ResearchConceptsIL-1βSpinal cordMechanical hyperalgesiaIntrathecal injectionIL-10Electronic von Frey apparatusAbility of gp120SOD expressionAnti-inflammatory actionIL-1β injectionMale Swiss miceIL-1 βIL-1β applicationGp120 administrationGp120 injectionAntihyperalgesic effectIntrathecal administrationMechanical sensitivityPrior administrationCytokine productionSwiss miceBlood samplesSterile salineHyperalgesiaPeripheral increaseAnti-inflammatory, antioxidant and anti-Mycobacterium tuberculosis activity of viridiflorol: The major constituent of Allophylus edulis (A. St.-Hil., A. Juss. & Cambess.) Radlk.
Trevizan L, do Nascimento K, Santos J, Kassuya C, Cardoso C, do Carmo Vieira M, Moreira F, Croda J, Formagio A. Anti-inflammatory, antioxidant and anti-Mycobacterium tuberculosis activity of viridiflorol: The major constituent of Allophylus edulis (A. St.-Hil., A. Juss. & Cambess.) Radlk. Journal Of Ethnopharmacology 2016, 192: 510-515. PMID: 27612433, DOI: 10.1016/j.jep.2016.08.053.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Inflammatory AgentsAntioxidantsAntitubercular AgentsBenzothiazolesBiphenyl CompoundsCarrageenanChemotaxis, LeukocyteDexamethasoneDisease Models, AnimalDose-Response Relationship, DrugEdemaFemaleGas Chromatography-Mass SpectrometryMaleMiceMycobacterium tuberculosisOils, VolatilePhytotherapyPicratesPlant ExtractsPlant LeavesPlant OilsPlants, MedicinalPleurisySapindaceaeSulfonic AcidsTerpenesTime FactorsConceptsNatural anti-inflammatory agentAnti-inflammatory agentsSubcutaneous injectionOral administrationAnti-Mycobacterium tuberculosis activityAnti-mycobacterial activityAntioxidant activityTotal leucocytesEOAETuberculosis activityMycobacterium tuberculosisGas chromatography-mass spectrometrySignificant inhibitionMiceTraditional medicineEdemaPleurisyPositive controlReference standardDexamethasoneCarrageenanAdministrationModerate antioxidant activityInjectionMajor constituents
2014
Evaluation of the anti-mycobacterium tuberculosis activity and in vivo acute toxicity of Annona sylvatic
Araujo R, Neves F, Formagio A, Kassuya C, Stefanello M, Souza V, Pavan F, Croda J. Evaluation of the anti-mycobacterium tuberculosis activity and in vivo acute toxicity of Annona sylvatic. BMC Complementary Medicine And Therapies 2014, 14: 209. PMID: 24974069, PMCID: PMC4082671, DOI: 10.1186/1472-6882-14-209.Peer-Reviewed Original ResearchConceptsVivo acute toxicityAnti-mycobacterial activityLC-MSFirst chemical characterizationAnti-Mycobacterium tuberculosis activityLiquid-liquid partitioningMinimal inhibitory concentrationEthyl acetate fractionChemical characterizationAntituberculosis activitySpectroscopic dataBody weightIsolation of quercetinAcute toxicityChemical fractionationChromatographic analysisFemale Swiss miceMultidrug-resistant Mycobacterium tuberculosis strainsControl of tuberculosisAcute toxicity evaluationToxicity evaluationMycobacterium tuberculosis strainsTuberculosis activityAlmunequinEAF fractionsAntihyperalgesic and antidepressive actions of (R)-(+)-limonene, α-phellandrene, and essential oil from Schinus terebinthifolius fruits in a neuropathic pain model
Piccinelli A, Santos J, Konkiewitz E, Oesterreich S, Formagio A, Croda J, Ziff E, Kassuya C. Antihyperalgesic and antidepressive actions of (R)-(+)-limonene, α-phellandrene, and essential oil from Schinus terebinthifolius fruits in a neuropathic pain model. Nutritional Neuroscience 2014, 18: 217-224. PMID: 24661285, DOI: 10.1179/1476830514y.0000000119.Peer-Reviewed Original ResearchConceptsCold hyperalgesiaMechanical hyperalgesiaAntidepressive actionOral treatmentLocomotor activityExhibit anti-inflammatory activityNerve injury modelNeuropathic pain modelDepressive-like behaviorDose of ketamineAnti-inflammatory activityOpen field testAntihyperalgesic effectNeuropathic painSNI ratsPain modelSwim testS. terebinthifolius fruitsInjury modelOral administrationHyperalgesiaSchinus terebinthifolius fruitsCold stimuliRatsTreatment
2011
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectin
Figueira CP, Croda J, Choy HA, Haake DA, Reis MG, Ko AI, Picardeau M. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology 2011, 11: 129. PMID: 21658265, PMCID: PMC3133549, DOI: 10.1186/1471-2180-11-129.Peer-Reviewed Original ResearchConceptsHeterologous expressionL. biflexaFunctional genetic analysisCultured cellsPathogen-specific proteinsVirulence-associated phenotypesSaprophytic L. biflexaPathogenesis of leptospirosisImmunoglobulin-like proteinEukaryotic cellsKey virulence factorsExtracellular matrix componentsLeptospiral immunoglobulin-like (Lig) proteinsSurrogate hostLig geneLeptospira biflexa serovar patocMolecular basisReplicative plasmidGenetic analysisLig proteinsSurface localizationCell adhesionSurface proteinsVirulence factorsLigB