2008
Resveratrol Inhibits Electrical Activity and Insulin Release from Insulinoma Cells by Block of Voltage-Gated Ca2+ Channels and Swelling-Dependent Cl- Currents
Jakab M, Lach S, Bacová Z, Langelüddecke C, Strbák V, Schmidt S, Iglseder E, Paulmichl M, Geibel J, Ritter M. Resveratrol Inhibits Electrical Activity and Insulin Release from Insulinoma Cells by Block of Voltage-Gated Ca2+ Channels and Swelling-Dependent Cl- Currents. Cellular Physiology And Biochemistry 2008, 22: 567-578. PMID: 19088439, DOI: 10.1159/000185541.Peer-Reviewed Original ResearchConceptsHigh extracellular glucoseElectrical activityInsulin releaseCl- currentBlock of VoltageWhole-cell patch-clamp experimentsPresence of iberiotoxinRat INS-1E cellsInsulinoma cellsMembrane depolarizationBasal insulin releaseGlucose-induced depolarizationExtracellular glucoseApplication of resveratrolCell membrane depolarizationPatch-clamp experimentsInsulin-Secreting CellsINS-1E cellsInsulin secretionRV doseAnimal modelsSecreting cellsInsulin responsivenessClamp experimentsResveratrol
2007
SLC26A7 Can function as a chloride-loading mechanism in parietal cells
Kosiek O, Busque SM, Föller M, Shcheynikov N, Kirchhoff P, Bleich M, Muallem S, Geibel JP. SLC26A7 Can function as a chloride-loading mechanism in parietal cells. Pflügers Archiv - European Journal Of Physiology 2007, 454: 989-998. PMID: 17404755, DOI: 10.1007/s00424-007-0254-y.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsAntiportersChloride-Bicarbonate AntiportersChloridesFluorescent Antibody TechniqueGastric AcidGastric MucosaImage Processing, Computer-AssistedIn Vitro TechniquesMaleOocytesParietal Cells, GastricPatch-Clamp TechniquesRatsRats, Sprague-DawleySodiumSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2Sulfate TransportersXenopusConceptsParietal cellsXenopus laevis oocyte expression systemRat parietal cellsExpression of SLC26A7Rat gastric glandsAcid secretionOocyte expression systemNKCC inhibitorWestern blotHistamine stimulationPharmacological inhibitorsAcidic pHiGastric glandsDIDS concentrationExchanger AE2Subsequent readditionTransporter activityDisulfonic acidInhibitorsCellsSLC26A7InfluxImmunohistochemistrySecretagoguesUptake system
2002
Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells
Egan ME, Glöckner-Pagel J, Ambrose C, Cahill PA, Pappoe L, Balamuth N, Cho E, Canny S, Wagner CA, Geibel J, Caplan MJ. Calcium-pump inhibitors induce functional surface expression of ΔF508-CFTR protein in cystic fibrosis epithelial cells. Nature Medicine 2002, 8: 485-492. PMID: 11984593, DOI: 10.1038/nm0502-485.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumCalcium pump inhibitorΔF508-CFTR proteinCystic fibrosis epithelial cellsCystic fibrosis transmembrane conductance regulator (CFTR) proteinCystic fibrosis cell lineFunctional surface expressionSurface expressionChaperone activityChaperone proteinsRegulator proteinPlasma membraneCystic fibrosis defectCell surfaceProteinCell linesPotential targetOptimal activityInhibitor thapsigarginEpithelial cellsExpressionCommon mutationsInhibitorsMouse modelReticulum
2001
Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis
Wagner C, Ott M, Klingel K, Beck S, Melzig J, Friedrich B, Wild K, Bröer S, Moschen I, Albers A, Waldegger S, Tümmler B, Egan M, Geibel J, Kandolf R, Lang F. Effects of the Serine/Threonine Kinase SGK1 on the Epithelial Na+ Channel (ENaC) and CFTR: Implications for Cystic Fibrosis. Cellular Physiology And Biochemistry 2001, 11: 209-218. PMID: 11509829, DOI: 10.1159/000051935.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAmino Acid SubstitutionAnimalsBronchiCell LineCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEpithelial CellsEpithelial Sodium ChannelsHumansIn Situ HybridizationLungMacrophages, AlveolarMutationOocytesPatch-Clamp TechniquesProtein Serine-Threonine KinasesPulmonary AlveoliRNA, ComplementaryRNA, MessengerSodiumSodium ChannelsXenopus laevisConceptsSerine/threonine kinase SGK1Lung tissueCystic fibrosisCF patientsKinase SGK1CF lung tissueXenopus oocytesLoss of CFTRLung epithelial cell lineCoexpression of CFTREffect of SGK1Pathophysiological factorsEpithelial cell lineRespiratory epitheliumLung phenotypeVariety of stimuliCl(-) secretionSGK1 expressionInhibitor amilorideInhibitory effectEpithelial cellsEnhanced expressionChannel ENaC.CFTR mutationsChannel activity
2000
Calcium, ATP and nuclear pore channel gating
Bustamante J, Michelette E, Geibel J, Dean D, Hanover J, McDonnell T. Calcium, ATP and nuclear pore channel gating. Pflügers Archiv - European Journal Of Physiology 2000, 439: 433-444. PMID: 10678739, PMCID: PMC4400178, DOI: 10.1007/s004249900189.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntibodies, MonoclonalBiological TransportCalciumCalcium ChannelsChelating AgentsCytosolDextransEgtazic AcidEndoplasmic ReticulumEnzyme InhibitorsFluorescein-5-isothiocyanateFluorescent DyesGene Expression Regulation, NeoplasticInositol 1,4,5-TrisphosphateIon Channel GatingMaleNuclear EnvelopeOocytesPatch-Clamp TechniquesProstatic NeoplasmsThapsigarginTumor Cells, CulturedXenopus laevisConceptsNPC channelGene activityChannel gatingMacromolecular transportCytosolic Ca2Fluorescence microscopy observationsTranscription factorsNE membranesProstate cancer cellsCytosolic ATPIntracellular messengerCellular rhythmsRole of Ca2ATPCancer cellsPatch-clamp studiesPresent patch-clamp studyRNAIon channel behaviorRegulationCardiomyocyte nucleiGatingExpressionCa2ThapsigarginDendrimer-assisted patch-clamp sizing of nuclear pores
Bustamante J, Michelette E, Geibel J, Hanover J, McDonnell T, Dean D. Dendrimer-assisted patch-clamp sizing of nuclear pores. Pflügers Archiv - European Journal Of Physiology 2000, 439: 829-837. PMID: 10784359, PMCID: PMC4397974, DOI: 10.1007/s004249900233.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear localization signalMacromolecular translocationFluorescence microscopy dataNuclear envelopeGreen fluorescence proteinLocalization signalPore complexNuclear poresMicroscopy dataColloidal gold particlesFluorescence proteinElectron microscopyCell lysatesFluorescence microscopyPhysiological conditionsDistinct classesCellular structureGold particlesProteinTranslocationUseful probeCardiomyocyte nucleiB-phycoerythrinParticles