2004
CXCR2 Blockade Influences Anaplasma phagocytophilum Propagation but Not Histopathology in the Mouse Model of Human Granulocytic Anaplasmosis
Scorpio DG, Akkoyunlu M, Fikrig E, Dumler JS. CXCR2 Blockade Influences Anaplasma phagocytophilum Propagation but Not Histopathology in the Mouse Model of Human Granulocytic Anaplasmosis. MSphere 2004, 11: 963-968. PMID: 15358660, PMCID: PMC515272, DOI: 10.1128/cdli.11.5.963-968.2004.Peer-Reviewed Original ResearchConceptsHuman granulocytic anaplasmosisControl miceGranulocytic anaplasmosisC3H-scid miceInfected cellsTissue loadsObligate intracellular bacteriumNeutrophil recruitmentNeutrophil secretionAntibody blockadeChemokine inductionHepatic pathologyLiver histopathologyInterleukin-8Tissue injuryMouse modelControl animalsDay 14Intracellular bacteriumMiceInfectionAnaplasma phagocytophilumA. phagocytophilumHistopathologyNeutrophils
2001
Exploitation of Interleukin-8-Induced Neutrophil Chemotaxis by the Agent of Human Granulocytic Ehrlichiosis
Akkoyunlu M, Malawista S, Anguita J, Fikrig E. Exploitation of Interleukin-8-Induced Neutrophil Chemotaxis by the Agent of Human Granulocytic Ehrlichiosis. Infection And Immunity 2001, 69: 5577-5588. PMID: 11500432, PMCID: PMC98672, DOI: 10.1128/iai.69.9.5577-5588.2001.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisInterleukin-8Granulocytic ehrlichiosisHGE bacteriaUpregulation of CXCR2IL-8 receptorsIL-8 productionIL-8 secretionSite of infectionInfected HL-60 cellsObligate intracellular bacteriumChemotaxis chamber assayNeutrophils migrateNeutrophil migrationNeutrophil chemotaxisImmunodeficient miceControl animalsBacterial disseminationHost defensePromyelocytic cell lineChamber assayIntracellular bacteriumHL-60 cellsRetinoic acidNeutrophil lineage
2000
Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase
Banerjee R, Anguita J, Fikrig E. Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase. Infection And Immunity 2000, 68: 4361-4362. PMID: 10858261, PMCID: PMC101771, DOI: 10.1128/iai.68.7.4361-4362.2000.Peer-Reviewed Original Research
1999
Human Granulocytic Ehrlichiosis in Western Europe
van Dobbenburgh A, van Dam A, Fikrig E. Human Granulocytic Ehrlichiosis in Western Europe. New England Journal Of Medicine 1999, 340: 1214-1216. PMID: 10206853, DOI: 10.1056/nejm199904153401517.Peer-Reviewed Original Research
1998
Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays
Magnarelli L, IJdo J, Dumler J, Heimer R, Fikrig E. Reactivity of Human Sera to Different Strains of Granulocytic Ehrlichiae in Immunodiagnostic Assays. The Journal Of Infectious Diseases 1998, 178: 1835-1838. PMID: 9815246, DOI: 10.1086/314516.Peer-Reviewed Original ResearchConceptsNCH-1 strainIndirect IFAHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisWestern blot analysisInfected neutrophilsPromyelocytic leukemia cellsHuman promyelocytic leukemia cellsAntibody detectionGranulocytic ehrlichiosisEhrlichia equiDifferent strainsHuman isolatesLeukemia cellsSerumImmunodiagnostic assaysBlot analysisGranulocytic ehrlichiaeIFA analysisHuman serumIFAEhrlichiaeAssaysSeropositivityPatientsCloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response
Ijdo J, Sun W, Zhang Y, Magnarelli L, Fikrig E. Cloning of the Gene Encoding the 44-Kilodalton Antigen of the Agent of Human Granulocytic Ehrlichiosis and Characterization of the Humoral Response. Infection And Immunity 1998, 66: 3264-3269. PMID: 9632594, PMCID: PMC108341, DOI: 10.1128/iai.66.7.3264-3269.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisAgent of HGESera of patientsBorrelia burgdorferi infectionMsp-2 genesElicit specific antibodiesHumoral responseBurgdorferi infectionMurine infectionHealthy individualsPatientsSerumSpecific antibodiesHL-60 cellsInfectionAntibodiesEhrlichia chaffeensisAntigenMiceEhrlichiosisGenomic DNA expression libraryMultigene familyGlutathione transferase fusion proteinFusion proteinGranulocytic Ehrlichiosis in the Laboratory Mouse
Hodzic E, IJdo J, Feng S, Katavolos P, Sun W, Maretzki C, Fish D, Fikrig E, Telford S, Barthold S. Granulocytic Ehrlichiosis in the Laboratory Mouse. The Journal Of Infectious Diseases 1998, 177: 737-745. PMID: 9498456, DOI: 10.1086/514236.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisMouse passageGranulocytic ehrlichiosisHL-60 cell culturesPeripheral bloodC3H miceLate infectionEarly infectionCell culturesDay 17Day 5Blood smearsNormal valuesDay 24Polymerase chain reaction amplificationHGE agentChain reaction amplificationSpleen smearsMiceInfectionInfectivity assaysBloodLaboratory miceSmearsReaction amplificationHeat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies.
Ijdo J, Zhang Y, Anderson M, Goldberg D, Fikrig E. Heat shock protein 70 of the agent of human granulocytic ehrlichiosis binds to Borrelia burgdorferi antibodies. MSphere 1998, 5: 118-20. PMID: 9455892, PMCID: PMC121403, DOI: 10.1128/cdli.5.1.118-120.1998.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisEhrlichial antigenHSP-70Borrelia burgdorferi antibodiesSerologic test resultsHeat shock protein 70Shock protein 70Burgdorferi antibodiesPatient seraGranulocytic ehrlichiosisLyme diseaseFirst weekHeat shock proteinsLyme disease spirochetePrior exposureImmunoglobulin GProtein 70Immunoblot analysisAntigenShock proteinsSerumAntibodiesPatientsIllnessIgG
1997
Immunization against the agent of human granulocytic ehrlichiosis in a murine model.
Sun W, IJdo JW, Telford SR, Hodzic E, Zhang Y, Barthold SW, Fikrig E. Immunization against the agent of human granulocytic ehrlichiosis in a murine model. Journal Of Clinical Investigation 1997, 100: 3014-3018. PMID: 9399947, PMCID: PMC508513, DOI: 10.1172/jci119855.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisGranulocytic ehrlichiosisC3H/HeN miceAgent of HGEMimic human diseaseTick-borne infectionsTransient neutropeniaHeN miceMurine modelPolymorphonuclear leukocytesTick-borne pathogensAoHGEMiceImmunityEhrlichiosisSyringe inoculationHuman diseasesAgentsNeutropeniaImmunizationInfectionLeukocytesDiseaseAntibodiesThe Early Humoral Response in Human Granulocytic Ehrlichiosis
IJdo J, Zhang Y, Hodzic E, Magnarelli L, Wilson M, Telford S, Barthold S, Fikrig E. The Early Humoral Response in Human Granulocytic Ehrlichiosis. The Journal Of Infectious Diseases 1997, 176: 687-692. PMID: 9291316, DOI: 10.1086/514091.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAntibody responseGranulocytic ehrlichiosisHGE agentC3H/HeJ miceEarly humoral responseEarly antibody responseStrong antibody responseHumoral responseHeJ miceClinical infectionPatientsAntigenHL-60 cellsMiceInfectionIgGEhrlichiosisSerumWeeksResponseIgMAgentsDiseaseDiagnosis