2008
The Tick Salivary Protein Salp15 Inhibits the Killing of Serum-Sensitive Borrelia burgdorferi Sensu Lato Isolates
Schuijt TJ, Hovius JW, van Burgel ND, Ramamoorthi N, Fikrig E, van Dam AP. The Tick Salivary Protein Salp15 Inhibits the Killing of Serum-Sensitive Borrelia burgdorferi Sensu Lato Isolates. Infection And Immunity 2008, 76: 2888-2894. PMID: 18426890, PMCID: PMC2446733, DOI: 10.1128/iai.00232-08.Peer-Reviewed Original ResearchConceptsComplement-mediated killingBurgdorferi-specific antibodiesBorrelia burgdorferiStrong protective effectSerum-sensitive isolatesNormal human serumBorrelia burgdorferi sensu latoBurgdorferi sensu latoProtective effectMembrane attack complexTick salivaLyme diseaseBorrelia burgdorferi sensu lato isolatesComplement complexI. scapularis Salp15B. burgdorferiSalp15Attack complexBurgdorferiHuman serumKillingSpirochetesIxodes scapularisIsolatesTicks
2007
Age-Associated Defect in Human TLR-1/2 Function
van Duin D, Mohanty S, Thomas V, Ginter S, Montgomery RR, Fikrig E, Allore HG, Medzhitov R, Shaw AC. Age-Associated Defect in Human TLR-1/2 Function. The Journal Of Immunology 2007, 178: 970-975. PMID: 17202359, DOI: 10.4049/jimmunol.178.2.970.Peer-Reviewed Original ResearchConceptsTLR1 surface expressionCytokine productionTLR functionTNF-alphaSurface expressionMultivariable mixed-effects modelsOlder adultsImpaired vaccine responsesTLR2 surface expressionInfection-related morbidityPeripheral blood monocytesYears of ageVaccine responsesIL-6Aged miceBlood monocytesYoung controlsFlow cytometryMixed effects modelsAge categoriesTLR1/2TLREffects modelN-palmitoylAdults
2004
Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection
Carlyon JA, Latif D, Pypaert M, Lacy P, Fikrig E. Anaplasma phagocytophilum Utilizes Multiple Host Evasion Mechanisms To Thwart NADPH Oxidase-Mediated Killing during Neutrophil Infection. Infection And Immunity 2004, 72: 4772-4783. PMID: 15271939, PMCID: PMC470610, DOI: 10.1128/iai.72.8.4772-4783.2004.Peer-Reviewed Original ResearchConceptsPhorbol myristate acetateUnique tropismA. phagocytophilumNADPH oxidase assemblyOxidative killingNADPH oxidaseAnaplasma phagocytophilumSerum-opsonized zymosanA. phagocytophilum infectionHost evasion mechanismNeutrophil infectionOxygen species productionEvasion mechanismsEtiologic agentNADPH oxidase complexHuman anaplasmosisNeutrophilsPhagocytophilum infectionSecondary activationMyristate acetateDependent decreaseInitial stimulationNeutrophil plasma membranesSpecies productionPhagocytophilum
2000
Cutting Edge: Infection by the Agent of Human Granulocytic Ehrlichiosis Prevents the Respiratory Burst by Down-Regulating gp91phox
Banerjee R, Anguita J, Roos D, Fikrig E. Cutting Edge: Infection by the Agent of Human Granulocytic Ehrlichiosis Prevents the Respiratory Burst by Down-Regulating gp91phox. The Journal Of Immunology 2000, 164: 3946-3949. PMID: 10754283, DOI: 10.4049/jimmunol.164.8.3946.Peer-Reviewed Original ResearchConceptsHGE bacteriaNADPH oxidase enzyme complexHL-60 cellsNADPH oxidaseHuman granulocytic ehrlichiosisEnzyme complexPromyelocytic cell lineTick-borne pathogensInfected cellsCell linesOrganismsMRNA levelsGp91phox proteinRT-PCRDirect inhibitionBacteriaFACS analysisPathogensMRNA expressionOxidaseGenerate superoxide anionCellsRespiratory burstSplenic neutrophilsMicrobes