2008
Preferential Protection of Borrelia burgdorferi Sensu Stricto by a Salp 15 Homologue in Ixodes ricinus Saliva
Hovius JW, Schuijt TJ, de Groot KA, Roelofs JJ, Oei GA, Marquart JA, de Beer R, Veer C, van der Poll T, Ramamoorthi N, Fikrig E, van Dam AP. Preferential Protection of Borrelia burgdorferi Sensu Stricto by a Salp 15 Homologue in Ixodes ricinus Saliva. The Journal Of Infectious Diseases 2008, 198: 1189-1197. PMID: 18752445, PMCID: PMC4317250, DOI: 10.1086/591917.Peer-Reviewed Original ResearchConceptsAntibody-mediated killingB. burgdorferiB. afzeliiB. gariniiEnzyme-linked immunosorbent assaySole causative agentBorrelia burgdorferi sensu latoBorrelia speciesSevere carditisOuter surface protein CImmune miceControl miceBorrelia burgdorferi sensu strictoBurgdorferi sensu latoSurface protein CSurvival advantageBurgdorferi sensu strictoSolid-phase overlayLyme borreliosisImmunosorbent assayProtein CCausative agentIxodes ticksBurgdorferiMice
2004
Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses
Liang FT, Yan J, Mbow ML, Sviat SL, Gilmore RD, Mamula M, Fikrig E. Borrelia burgdorferi Changes Its Surface Antigenic Expression in Response to Host Immune Responses. Infection And Immunity 2004, 72: 5759-5767. PMID: 15385475, PMCID: PMC517580, DOI: 10.1128/iai.72.10.5759-5767.2004.Peer-Reviewed Original ResearchConceptsSurface antigenic expressionAntigenic expressionImmune attackImmune responseSevere combined immunodeficiency miceHostile immune environmentVigorous immune responseB. burgdorferiCombined immunodeficiency miceHost immune responseOspC antibodiesOuter surface protein CImmune environmentDecorin-binding protein AImmunocompetent miceImmune conditionsImmunodeficiency miceSurface protein CImmune pressureVlsE expressionLyme disease spirocheteProtein CBorrelia burgdorferiOspC expressionMammalian infectionOspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
Pal U, Yang X, Chen M, Bockenstedt LK, Anderson JF, Flavell RA, Norgard MV, Fikrig E. OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands. Journal Of Clinical Investigation 2004, 113: 220-230. PMID: 14722614, PMCID: PMC311436, DOI: 10.1172/jci19894.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAntigens, BacterialBacterial Outer Membrane ProteinsBorrelia burgdorferiDisease Models, AnimalEnzyme-Linked Immunosorbent AssayGenetic VectorsImmunoglobulin Fab FragmentsIxodesLipoproteinsLyme DiseaseMiceMice, SCIDMicroscopy, ConfocalModels, GeneticPlasmidsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSalivary GlandsSpirochaetalesTime FactorsUp-RegulationConceptsTick salivary glandsWild-type B. burgdorferiArthropod vectorsWild-type spirochetesTransmission of spirochetesB. burgdorferiMajor surface lipoproteinMammalian hostsGene expressionSalivary glandsOspC geneSurface lipoproteinsSalivary gland colonizationInfectious cloneOuter surface protein CGland colonizationSurface protein CTick gutMutantsOspCUnfed ticksInvasionCritical stepBorrelia burgdorferiSpirochetes
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1999
The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis
Akin E, McHugh G, Flavell R, Fikrig E, Steere A. The Immunoglobulin (IgG) Antibody Response to OspA and OspB Correlates with Severe and Prolonged Lyme Arthritis and the IgG Response to P35 Correlates with Mild and Brief Arthritis. Infection And Immunity 1999, 67: 173-181. PMID: 9864212, PMCID: PMC96293, DOI: 10.1128/iai.67.1.173-181.1999.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAntigens, BacterialAntigens, SurfaceArthritis, InfectiousBacterial Outer Membrane ProteinsBacterial ProteinsBacterial VaccinesBorrelia burgdorferi GroupChildChild, PreschoolFemaleHumansImmunoglobulin GLipoproteinsLongitudinal StudiesLyme DiseaseMaleMiddle AgedSeverity of Illness IndexConceptsAntibody responseIgG antibodiesEarly arthritisLyme arthritisIgG responsesEarly infectionDuration of arthritisChronic Lyme arthritisIgG antibody responseSerial serum samplesBorrelia burgdorferi proteinsSubsequent arthritisB. burgdorferi proteinsSurface protein CC-terminal epitopeImmune responseArthritisSubsequent severityNatural historyLyme diseasePatientsSerum samplesImmunoglobulin GProtein CInfection