2023
Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
Mazuecos L, Alberdi P, Hernández-Jarguín A, Contreras M, Villar M, Cabezas-Cruz A, Simo L, González-García A, Díaz-Sánchez S, Neelakanta G, Bonnet S, Fikrig E, de la Fuente J. Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector. IScience 2023, 26: 106697. PMID: 37168564, PMCID: PMC10165458, DOI: 10.1016/j.isci.2023.106697.Peer-Reviewed Original ResearchHuman granulocytic anaplasmosisPathogen infection/transmissionTick-borne pathogensTick-borne diseasesInfection/transmissionTick vectorGranulocytic anaplasmosisWildtype populationTick microbiotaPathogensModel pathogenTransovarialAnaplasmosisMSP4TicksAssociated reductionCompetitionLarvaeDisease riskParatransgenesisSymbiontsInfectionCommensal bacteriaBacteriaControl
2017
Infection-derived lipids elicit an immune deficiency circuit in arthropods
Shaw DK, Wang X, Brown LJ, Chávez AS, Reif KE, Smith AA, Scott AJ, McClure EE, Boradia VM, Hammond HL, Sundberg EJ, Snyder GA, Liu L, DePonte K, Villar M, Ueti MW, de la Fuente J, Ernst RK, Pal U, Fikrig E, Pedra JH. Infection-derived lipids elicit an immune deficiency circuit in arthropods. Nature Communications 2017, 8: 14401. PMID: 28195158, PMCID: PMC5316886, DOI: 10.1038/ncomms14401.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnaplasma marginaleAnaplasma phagocytophilumAnimalsArthropodsBorrelia burgdorferiCarrier ProteinsDisease Models, AnimalDrosophila melanogasterDrosophila ProteinsEscherichia coliFas-Associated Death Domain ProteinGene SilencingHEK293 CellsHumansImmunologic Deficiency SyndromesIxodesLipidsLyme DiseasePhosphatidylglycerolsRecombinant ProteinsRNA, Small InterferingSignal TransductionTranscription FactorsUbiquitin-Conjugating EnzymesUbiquitin-Protein LigasesX-Linked Inhibitor of Apoptosis ProteinConceptsAdaptor molecule FasImmune deficiency (IMD) pathwayPeptidoglycan recognition proteinsE3 ubiquitin ligaseLyme disease spirochete Borrelia burgdorferiIMD pathwayGram-negative bacteriaRecognition proteinsUbiquitin ligaseDeath domainApoptosis proteinDistinct bacteriaBiochemical interactionsMolecule FasSpirochete Borrelia burgdorferiReceptor networkA. marginaleProteinAnaplasma phagocytophilumBacteriaPathwayBorrelia burgdorferiInsectsLipidsArthropods
2015
Tick microbiome: the force within
Narasimhan S, Fikrig E. Tick microbiome: the force within. Trends In Parasitology 2015, 31: 315-323. PMID: 25936226, PMCID: PMC4492851, DOI: 10.1016/j.pt.2015.03.010.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2014
Antivirulence Properties of an Antifreeze Protein
Heisig M, Abraham NM, Liu L, Neelakanta G, Mattessich S, Sultana H, Shang Z, Ansari JM, Killiam C, Walker W, Cooley L, Flavell RA, Agaisse H, Fikrig E. Antivirulence Properties of an Antifreeze Protein. Cell Reports 2014, 9: 417-424. PMID: 25373896, PMCID: PMC4223805, DOI: 10.1016/j.celrep.2014.09.034.Peer-Reviewed Original ResearchConceptsAntifreeze proteinsDiverse bacteriaProtein bindsWild-type animalsBiofilm formationAntivirulence agentsIAFGPMethicillin-resistant Staphylococcus aureusHost controlProteinAntifreeze glycoproteinsIxodes scapularisAntivirulence propertiesBacteriaSeptic shockTherapeutic strategiesBacterial infectionsInfectious diseasesMicrobesStaphylococcus aureusFliesBindsInfectionCatheter tubingPathogens
2002
Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis
IJdo JW, Wu C, Telford SR, Fikrig E. Differential Expression of the p44 Gene Family in the Agent of Human Granulocytic Ehrlichiosis. Infection And Immunity 2002, 70: 5295-5298. PMID: 12183586, PMCID: PMC128253, DOI: 10.1128/iai.70.9.5295-5298.2002.Peer-Reviewed Original ResearchConceptsP44 geneDifferential expressionHuman granulocytic ehrlichiosisGene familyAntigenic variationHGE bacteriaTransmission feedingGranulocytic ehrlichiosisGenesHypervariable regionInfected C3H miceP44 expressionTick transmissionExpressionSCID miceC3H miceHGE infectionMurine modelInfected ticksSalivary glandsNymphal ticksMiceTicksEhrlichiosisBacteria
2000
Cutting Edge: Infection by the Agent of Human Granulocytic Ehrlichiosis Prevents the Respiratory Burst by Down-Regulating gp91phox
Banerjee R, Anguita J, Roos D, Fikrig E. Cutting Edge: Infection by the Agent of Human Granulocytic Ehrlichiosis Prevents the Respiratory Burst by Down-Regulating gp91phox. The Journal Of Immunology 2000, 164: 3946-3949. PMID: 10754283, DOI: 10.4049/jimmunol.164.8.3946.Peer-Reviewed Original ResearchConceptsHGE bacteriaNADPH oxidase enzyme complexHL-60 cellsNADPH oxidaseHuman granulocytic ehrlichiosisEnzyme complexPromyelocytic cell lineTick-borne pathogensInfected cellsCell linesOrganismsMRNA levelsGp91phox proteinRT-PCRDirect inhibitionBacteriaFACS analysisPathogensMRNA expressionOxidaseGenerate superoxide anionCellsRespiratory burstSplenic neutrophilsMicrobes
1998
The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment.
Webster P, IJdo JW, Chicoine LM, Fikrig E. The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment. Journal Of Clinical Investigation 1998, 101: 1932-1941. PMID: 9576758, PMCID: PMC508780, DOI: 10.1172/jci1544.Peer-Reviewed Original ResearchConceptsEndocytic pathwayLysosomal membrane glycoproteins lamp-1Membrane-bound compartmentsMannose-6-phosphate receptorInfected host cellsHL-60 cellsEndosomal compartmentsVacuole membraneLAMP-1Host cellsHGE bacteriaVacuolesTransferrin receptorColloidal gold particlesCompartmentsPathwayCytoplasmic vacuolesCellsAgent of HGEReceptorsOrganismsLow pHHuman granulocytic ehrlichiosisBacteriaHGE agent