2013
Hepatitis C Virus RNA Replication and Virus Particle Assembly Require Specific Dimerization of the NS4A Protein Transmembrane Domain
Kohlway A, Pirakitikulr N, Barrera FN, Potapova O, Engelman DM, Pyle AM, Lindenbach BD. Hepatitis C Virus RNA Replication and Virus Particle Assembly Require Specific Dimerization of the NS4A Protein Transmembrane Domain. Journal Of Virology 2013, 88: 628-642. PMID: 24173222, PMCID: PMC3911751, DOI: 10.1128/jvi.02052-13.Peer-Reviewed Original ResearchConceptsTM domainVirus particle assemblyRNA replicationTransmembrane protein essentialProtein transmembrane domainProtein-protein interactionsProtein interaction systemForward geneticsVirus RNA replicationParticle assemblySpecific dimerizationTransmembrane domainHuman blood group antigensProtein essentialTM helicesMutational effectsHomotypic interactionsDimer interfacePotential dimerizationTM interactionsVirus assemblyNonstructural proteinsDimeric interactionsHepatitis C Virus RNA ReplicationMutations
2011
Structural Insights into RNA Recognition by RIG-I
Luo D, Ding SC, Vela A, Kohlway A, Lindenbach BD, Pyle AM. Structural Insights into RNA Recognition by RIG-I. Cell 2011, 147: 409-422. PMID: 22000018, PMCID: PMC3222294, DOI: 10.1016/j.cell.2011.09.023.Peer-Reviewed Original ResearchConceptsPathogen-associated molecular patternsTerminal regulatory domainProtein familyRegulatory domainProtein domainsInsertion domainRNA recognitionMolecular basisATP hydrolysisStructural insightsIntracellular RIGRNAMolecular patternsMotor domainLike receptorsAntiviral immune responseViral RNANanomechanical FunctionDomainHelicasesComplex interplayHelicaseCladeHel2DsRNA
1999
Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function
Lindenbach B, Rice C. Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function. Journal Of Virology 1999, 73: 4611-4621. PMID: 10233920, PMCID: PMC112502, DOI: 10.1128/jvi.73.6.4611-4621.1999.Peer-Reviewed Original ResearchConceptsNonstructural protein 1Genetic interactionsRNA replicationViral RNA replicationGenetic screenSuppressor mutantsExtracytoplasmic compartmentsReplicase functionCytoplasmic complexDeletion mutantsReplicase componentsSingle mutationYellow fever virusProtein 1Additional mutationsNS1 geneRelated virusesInfected cellsIndependent selectionNS4A genesMutantsMutationsGenesReplicationDengue virus
1998
Noncytopathic Sindbis virus RNA vectors for heterologous gene expression
Agapov E, Frolov I, Lindenbach B, Prágai B, Schlesinger S, Rice C. Noncytopathic Sindbis virus RNA vectors for heterologous gene expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 12989-12994. PMID: 9789028, PMCID: PMC23682, DOI: 10.1073/pnas.95.22.12989.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GalactosidaseCell LineCloning, MolecularCricetinaeDNA-Directed RNA PolymerasesElectroporationGenes, ReporterGenetic VectorsGreen Fluorescent ProteinsKidneyLuminescent ProteinsPlasmidsRecombinant ProteinsRepliconRNA, ViralSindbis VirusTranscription, GeneticTransfectionViral ProteinsConceptsForeign gene expressionGene expressionLong-term foreign gene expressionSindbis vectorsHeterologous gene expressionDefective interfering genomesVirus-encoded genesHost protein synthesisVertebrate cellsHeterologous genesRecombinant Sindbis virusForeign genesDrug puromycinCellular metabolismProtein synthesisDNA transfectionRNA vectorSubgenomic transcriptsForeign proteinsRapid expressionBHK cellsGenesRepliconSindbis virusDramatic inhibition
1997
trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication
Lindenbach B, Rice C. trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication. Journal Of Virology 1997, 71: 9608-9617. PMID: 9371625, PMCID: PMC230269, DOI: 10.1128/jvi.71.12.9608-9617.1997.Peer-Reviewed Original Research
1994
Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini
Lin C, Lindenbach B, Prágai B, McCourt D, Rice C. Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. Journal Of Virology 1994, 68: 5063-5073. PMID: 7518529, PMCID: PMC236449, DOI: 10.1128/jvi.68.8.5063-5073.1994.Peer-Reviewed Original Research