2005
The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylation
2003
Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignment
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression
1997
Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites
Sumpio B, Chang R, Xu W, Wang X, Du W. Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites. American Journal Of Physiology 1997, 273: c1441-c1448. PMID: 9374627, DOI: 10.1152/ajpcell.1997.273.5.c1441.Peer-Reviewed Original ResearchAnimalsAorta, ThoracicBase SequenceBinding SitesCattleCell CycleCell NucleusCells, CulturedChloramphenicol O-AcetyltransferaseCyclic AMP Response Element-Binding ProteinDNA-Binding ProteinsEndothelium, VascularGene Expression Regulation, EnzymologicHumansKineticsMutagenesis, Site-DirectedOligodeoxyribonucleotidesPromoter Regions, GeneticRecombinant Fusion ProteinsStress, MechanicalTime FactorsTissue Plasminogen ActivatorTranscription Factor AP-2Transcription FactorsTransfectionTissue plasminogen activator-biologic perspective for surgeons.
Chang R, Powell RJ, Sumpio BE. Tissue plasminogen activator-biologic perspective for surgeons. Journal Of The American College Of Surgeons 1997, 184: 529-39. PMID: 9145078.Peer-Reviewed Original Research