2005
Differential responsiveness of early- and late-passage endothelial cells to shear stress
Kudo FA, Warycha B, Juran PJ, Asada H, Teso D, Aziz F, Frattini J, Sumpio BE, Nishibe T, Cha C, Dardik A. Differential responsiveness of early- and late-passage endothelial cells to shear stress. The American Journal Of Surgery 2005, 190: 763-769. PMID: 16226955, DOI: 10.1016/j.amjsurg.2005.07.017.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAortaApoptosisBlotting, WesternCattleCell CountCell DivisionCell ProliferationCells, CulturedEndothelium, VascularIn Vitro TechniquesMuscle, Smooth, VascularPhosphorylationProliferating Cell Nuclear AntigenProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktShear StrengthStress, MechanicalTumor Suppressor Protein p53ConceptsLate passage endothelial cellsOrbital shear stressEarly passage cellsSmooth muscle cell migrationMuscle cell migrationEndothelial cellsSenescence modelAkt phosphorylationCell migrationProtein kinase B activationPassage cellsKinase B activationCell proliferationVascular disease increasesLate passage cellsBovine aortic endothelial cellsNuclear antigen reactivityAortic endothelial cellsEndothelial cell proliferationNeointimal hyperplasiaAntigen reactivityTotal AktBoyden chamberB activationWestern blottingDifferential effects of orbital and laminar shear stress on endothelial cells
Dardik A, Chen L, Frattini J, Asada H, Aziz F, Kudo FA, Sumpio BE. Differential effects of orbital and laminar shear stress on endothelial cells. Journal Of Vascular Surgery 2005, 41: 869-880. PMID: 15886673, DOI: 10.1016/j.jvs.2005.01.020.Peer-Reviewed Original Research
2004
Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway
Li W, Sumpio BE. Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. AJP Heart And Circulatory Physiology 2004, 288: h1591-h1597. PMID: 15591103, DOI: 10.1152/ajpheart.00382.2004.Peer-Reviewed Original ResearchMeSH Keywords3-Phosphoinositide-Dependent Protein KinasesAndrostadienesAnimalsAntibiotics, AntineoplasticAortaCarrier ProteinsCattleCell DivisionCells, CulturedChromonesEndothelium, VascularEnzyme InhibitorsFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorpholinesPhosphatidylinositol 3-KinasesPhosphodiesterase InhibitorsPhosphoinositide-3 Kinase InhibitorsPhosphoproteinsPhosphorylationProtein KinasesProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRibosomal Protein S6 KinasesSignal TransductionSirolimusTOR Serine-Threonine KinasesWortmanninConceptsVascular endothelial cell proliferationEndothelial cell proliferationStrain-induced activationSignal pathwayEC proliferationPD 98059Cell proliferationPI3K inhibitor wortmanninPI3K inhibitorsCycles/minExtracellular signal-regulated kinases 1Inhibitor PD 98059MTOR pathwaySignal-regulated kinases 1Bovine aortic ECsMammalian targetMTOR-4EK inhibitorsEukaryotic initiation factor 4EAortic ECsInitiation factor 4EMEK1 inhibitor PD 98059K activationProliferationRapamycinCell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases
Lee T, Sumpio BE. Cell signalling in vascular cells exposed to cyclic strain: the emerging role of protein phosphatases. Biotechnology And Applied Biochemistry 2004, 39: 129-139. PMID: 15032733, DOI: 10.1042/ba20030104.Peer-Reviewed Original ResearchConceptsPhosphorylation stateSerine/threonine residuesProtein phosphatase type 2AAbnormal phosphorylation stateCritical cellular functionsPhosphorylation of tyrosineRole of phosphatasesVascular cellsRole of proteinsThreonine residuesCellular functionsProtein kinaseIntracellular proteinsCellular growthHuman diseasesProteinPhosphataseType 2AEndothelial cellsCellsCardiac hypertrophyKinasePhosphorylationHaemodynamic forcesRole
2003
Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins
Zhang J, Li W, Sanders MA, Sumpio BE, Asit P, Basson MD. Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins. The FASEB Journal 2003, 17: 1-22. PMID: 12626437, DOI: 10.1096/fj.02-0663fje.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell AdhesionCell DivisionCell LineCollagen Type ICollagen Type IVEnzyme ActivationEpithelial CellsExtracellular Matrix ProteinsFibronectinsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesHumansIntegrin alpha5Integrin alphaVIntestinal MucosaLamininMitogen-Activated Protein KinasesPhosphorylationProtein-Tyrosine KinasesStress, MechanicalConceptsExtracellular signal-regulated protein kinaseMatrix proteinsIntestinal epithelial responsesHuman intestinal epithelial proliferationSignal-regulated protein kinaseJun N-terminal kinaseFocal adhesion kinaseIntestinal epithelial biologyMatrix-dependent mannerN-terminal kinaseExtracellular matrix proteinsEpithelial responseCaco-2 proliferationCaco-2BBe cellsIntestinal epithelial proliferationAdhesion kinaseEpidermal growth factorProtein kinaseERK activationEpithelial biologyEpithelial cell proliferationIntegrin subunitsKinaseAnti-integrin antibodiesMEK blockadeInvolvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells
Li W, Chen Q, Mills I, Sumpio BE. Involvement of S6 kinase and p38 mitogen activated protein kinase pathways in strain‐induced alignment and proliferation of bovine aortic smooth muscle cells. Journal Of Cellular Physiology 2003, 195: 202-209. PMID: 12652647, DOI: 10.1002/jcp.10230.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDNA-Binding ProteinsEnzyme InhibitorsImidazolesImmunosuppressive AgentsMAP Kinase Signaling SystemMitogen-Activated Protein KinasesMuscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesPyridinesRegional Blood FlowRibosomal Protein S6 KinasesSirolimusStress, MechanicalTranscription FactorsConceptsAortic smooth muscle cellsBovine aortic smooth muscle cellsKinase pathwaySmooth muscle cell phenotypeSmooth muscle cellsStrain-induced proliferationBovine aortic SMCS6 kinaseMuscle cell phenotypeSpecific inhibitorProliferation of SMCsActivation of p38SMC proliferationAortic SMCsMuscle cellsSMC alignment
2002
Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells
Lee DM, Gasparro FP, Wang XJ, Kopec C, DeLeo K, Sumpio BE. Photochemotherapy of vascular cells with 8‐methoxypsoralen and visible light: differential effects on endothelial and smooth muscle cells. Photodermatology Photoimmunology & Photomedicine 2002, 18: 244-252. PMID: 12390666, DOI: 10.1034/j.1600-0781.2002.02770.x.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsEndothelial cellsPercutaneous transluminal coronary angioplastyMuscle cellsTransluminal coronary angioplastyLong-term efficacyAortic smooth muscle cellsBovine aortic smooth muscle cellsDose-dependent fashionReversible inhibitionCoronary angioplastyIntermediate doseLow doseHigh doseSMC proliferationCell countVascular cellsDoseProliferation of ECsEC proliferationDifferential effectsProliferationCellular migrationPresent studySignificant effectInhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols
Araim O, a J, Waterhouse AL, Sumpio BE. Inhibition of vascular smooth muscle cell proliferation with red wine and red wine polyphenols. Journal Of Vascular Surgery 2002, 35: 1226-1232. PMID: 12042735, DOI: 10.1067/mva.2002.124358.Peer-Reviewed Original ResearchConceptsRed wine polyphenol extractVascular SMC proliferationRed wine polyphenolsVascular smooth muscle cell proliferationSmooth muscle cell proliferationMuscle cell proliferationSMC proliferationAtherosclerotic diseaseBeneficial effectsWine polyphenolsVascular SMCsTrypan blue exclusion studiesDealcoholized red wineRed wine consumptionCell proliferationPolyphenol extractDose-dependent fashionInhibits SMC proliferationPotential beneficial effectsObserved beneficial effectsBovine aortic SMCDehydrogenase cytotoxicityAortic SMCsInhibitory effectRed wineSPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM
THAMILSELVAN V, LI W, SUMPIO BE, BASSON MD. SPHINGOSINE-1-PHOSPHATE STIMULATES HUMAN CACO-2 INTESTINAL EPITHELIAL PROLIFERATION VIA p38 ACTIVATION AND ACTIVATES ERK BY AN INDEPENDENT MECHANISM. In Vitro Cellular & Developmental Biology - Animal 2002, 38: 246-253. PMID: 12197778, DOI: 10.1290/1071-2690(2002)038<0246:spshci>2.0.co;2.Peer-Reviewed Original ResearchConceptsExtracellular signal-regulated kinases 1Mitogen-activated protein kinaseMAP kinase kinaseCaco-2 proliferationMAPK activationHuman intestinal epithelial proliferationP38 activationCell typesSignal-regulated kinases 1Role of ERKMitogenic effectCaco-2 intestinal epithelial cellsIntracellular second messengerMEK inhibitionP38 MAPK activationCancer cell invasionKinase kinaseHuman Caco-2 intestinal epithelial cellsProtein kinaseStimulation of proliferationCell motilityIntestinal epithelial cell proliferationInhibitor PD98059ERK2ERK activation
2001
Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells
Li W, Duzgun A, Sumpio B, Basson M. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. AJP Gastrointestinal And Liver Physiology 2001, 280: g75-g87. PMID: 11123200, DOI: 10.1152/ajpgi.2001.280.1.g75.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell DivisionCell MovementCytoskeletal ProteinsEnzyme InhibitorsExtracellular MatrixFlavonoidsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression Regulation, EnzymologicHumansImidazolesIntegrinsIntestinesMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNaphthalenesP38 Mitogen-Activated Protein KinasesPaxillinPhosphoproteinsPhosphorylationProtein-Tyrosine KinasesPyridinesStress, MechanicalTransfectionConceptsFocal adhesion kinaseMAPK extracellular signal-regulated kinaseCaco-2 cellsMitogen-activated protein kinase activationVitro kinase assaysProtein kinase activationSignal-regulated kinaseMitogenic effectProtein kinase C inhibitionBeta1 integrin subunitsCaco-2 proliferationKinase assaysAdhesion kinaseFAK activationERK signalsJNK1 activationKinase activationTerminal kinaseMAPK activationUpstream signalsC-JunFAK inhibitionP38 inhibitionIntegrin subunitsKinase
2000
Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive formEffects of glutamine isomers on human (Caco-2) intestinal epithelial proliferation, strain-responsiveness, and differentiation
Murnin M, Kumar A, di Li G, Brown M, Sumpio B, Basson M. Effects of glutamine isomers on human (Caco-2) intestinal epithelial proliferation, strain-responsiveness, and differentiation. Journal Of Gastrointestinal Surgery 2000, 4: 435-442. PMID: 11058864, DOI: 10.1016/s1091-255x(00)80025-6.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell CountCell DifferentiationCell DivisionDietary SupplementsDipeptidyl-Peptidases and Tripeptidyl-PeptidasesEnteral NutritionEnterocytesEnzyme InhibitorsEpithelial CellsFastingGamma-GlutamyltransferaseGastrointestinal MotilityGlutamineHumansIntestinal MucosaIsomerismIsoxazolesPeristalsisStatistics, NonparametricStress, MechanicalConceptsGlutamine supplementationIntestinal epithelial cellsBowel motilityGut mucosaCaco-2 proliferationEpithelial cellsCaco-2L-glutamine supplementationGlutamine metabolismCaco-2 intestinal epithelial cellsIntestinal epithelial proliferationFrequency-dependent mannerHuman Caco-2 intestinal epithelial cellsCaco-2 cellsEnteral feedingL-glutamineHT-29 cellsVillous motilitySelective antagonistEpithelial proliferationLess potencyVivo frequencyMalignant colonocytesHuman colonocytesMaximal stimulationInhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis
Willis A, Fuse S, Wang X, Chen E, Tuszynski G, Sumpio B, Gahtan V. Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2000, 31: 1160-1167. PMID: 10842153, DOI: 10.1067/mva.2000.106489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell CountCell DivisionCells, CulturedChemotaxisChromonesEnzyme InhibitorsExtracellular Matrix ProteinsFibronectinsHyperplasiaIndolesMaleimidesMorpholinesMuscle, Smooth, VascularPhosphoinositide-3 Kinase InhibitorsProtein Kinase CSignal TransductionStatistics as TopicThrombospondin 1Tunica IntimaVitronectinConceptsProtein kinase CVSMC migrationLY-294002Thrombospondin-1ECM proteinsKinase CSmooth muscle cell chemotaxisSerum-free mediumCellular signaling pathwaysInhibition of phosphatidylinositolVascular smooth muscle cell chemotaxisVascular smooth muscle cell migrationPI3K inhibitorsSmooth muscle cell migrationSignaling pathwaysMuscle cell migrationBisindolylmaleimide ICell migrationPKC inhibitorGF 109203XExtracellular matrix depositionTop wellsK inhibitorsAortic VSMCsCell chemotaxis
1999
Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1998
Antiproliferative effect of elevated glucose in human microvascular endothelial cells
Kamal K, Du W, Mills I, Sumpio B. Antiproliferative effect of elevated glucose in human microvascular endothelial cells. Journal Of Cellular Biochemistry 1998, 71: 491-501. PMID: 9827695, DOI: 10.1002/(sici)1097-4644(19981215)71:4<491::aid-jcb4>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsMicrovascular endothelial cellsElevated glucoseHMEC-1Endothelial cellsAntiproliferative effectsDiabetic microangiopathyDiabetic foot ulcerationHuman dermal microvascular endothelial cellsCyclic AMP accumulationMicrovascular endothelial cell lineDermal microvascular endothelial cellsHuman microvascular endothelial cellsImmortalized human dermal microvascular endothelial cell lineHigh D-glucoseDeleterious effectsHuman dermal microvascular endothelial cell linePrimary cultured cellsEndothelial cell lineFoot ulcerationHMEC-1 proliferationProliferative responseAMP accumulationPathological complicationsMolecular pathophysiologyRp-cAMPSStrain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals
Han O, Di Li G, Sumpio B, Basson M. Strain induces Caco-2 intestinal epithelial proliferation and differentiation via PKC and tyrosine kinase signals. American Journal Of Physiology 1998, 275: g534-g541. PMID: 9724266, DOI: 10.1152/ajpgi.1998.275.3.g534.Peer-Reviewed Original ResearchMolecular Basis for Tissue Expansion: Clinical Implications for the Surgeon
Takei T, Mills I, Arai K, Sumpio B. Molecular Basis for Tissue Expansion: Clinical Implications for the Surgeon. Plastic & Reconstructive Surgery 1998, 102: 247-258. PMID: 9655439, DOI: 10.1097/00006534-199807000-00044.Peer-Reviewed Original ResearchThe differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro
Sawmiller C, Powell R, Quader M, Dudrick S, Sumpio B. The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro. Journal Of Vascular Surgery 1998, 27: 1128-1140. PMID: 9652475, DOI: 10.1016/s0741-5214(98)70015-1.Peer-Reviewed Original ResearchConceptsNonionic contrastEndothelial cellsIonic contrastSmooth muscle cell proliferationSignificant decreaseSmooth muscle cell growthNonionic contrast agentsCultured bovine aorta endothelial cellsMuscle cell proliferationBrief exposureMuscle cell growthRole of osmolalityContrast exposureEC injuryContrast agentsAorta endothelial cellsLength of exposureDay 3SMC proliferationOsmolar effectsBovine aorta endothelial cellsEffect of contrastCell proliferationConfluent endothelial cellsOsmolar solutionInduction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change
Takei T, Kito H, Du W, Mills I, Sumpio B. Induction of interleukin (IL)‐1α and β gene expression in human keratinocytes exposed to repetitive strain: Their role in strain‐induced keratinocyte proliferation and morphological change. Journal Of Cellular Biochemistry 1998, 69: 95-103. PMID: 9548558, DOI: 10.1002/(sici)1097-4644(19980501)69:2<95::aid-jcb1>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsIL-1 antibodyIL-1 alphaIL-1Keratinocyte proliferationIL-1 mRNA expressionKeratinocyte biologyIL-1 gene expressionInduction of interleukinIL-1 betaIL-1 expressionMorphological changesΒ gene expressionAutocrine regulationMRNA expressionBeta mRNAGene expressionBasal rateBasic cellular propertiesHuman keratinocytesAntibodiesKeratinocyte functionRepetitive strainNorthern blot analysisBlot analysisProliferationAmbient Pulsatile Pressure Modulates Endothelial Cell Proliferation
Vouyouka A, Powell R, Ricotta J, Chen H, Dudrick D, Sawmiller C, Dudrick S, Sumpio B. Ambient Pulsatile Pressure Modulates Endothelial Cell Proliferation. Journal Of Molecular And Cellular Cardiology 1998, 30: 609-615. PMID: 9515036, DOI: 10.1006/jmcc.1997.0625.Peer-Reviewed Original Research