2008
Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2005
Negative Regulation of Monocyte Adhesion to Arterial Elastic Laminae by Signal Regulatory Protein α and Src Homology 2 Domain-containing Protein-Tyrosine Phosphatase-1*
Liu SQ, Alkema PK, Tieché C, Tefft BJ, Liu DZ, Li YC, Sumpio BE, Caprini JA, Paniagua M. Negative Regulation of Monocyte Adhesion to Arterial Elastic Laminae by Signal Regulatory Protein α and Src Homology 2 Domain-containing Protein-Tyrosine Phosphatase-1*. Journal Of Biological Chemistry 2005, 280: 39294-39301. PMID: 16159885, DOI: 10.1074/jbc.m503866200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesCell AdhesionElastic TissueIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsLeukocytesMonocytesPhosphorylationProtein Phosphatase 1Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesRatsRats, Sprague-DawleyReceptors, Cell SurfaceRNA, Small InterferingConceptsSHP-1Arterial elastic laminaeSrc homology 2 domain-containing protein tyrosine phosphataseSIRP-alphaSrc homology 2 domain-containing protein tyrosine phosphatase 1Protein tyrosine phosphatase 1Protein tyrosine phosphataseSignal regulatory protein alphaSignal regulatory protein αRegulatory protein alphaExtracellular matrix constituentsMonocyte adhesionArterial morphogenesisPhosphatase 1Regulatory protein αNegative regulationProtein alphaProtein αImportant functionsInduced activationDegradation peptidesInhibitory effectElasticity of arteriesAdhesionMatrix constituentsThe role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylation
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpressionCyclic Strain Stimulates Early Growth Response Gene Product 1–Mediated Expression of Membrane Type 1 Matrix Metalloproteinase in Endothelium
Yamaguchi S, Yamaguchi M, Yatsuyanagi E, Yun SS, Nakajima N, Madri JA, Sumpio BE. Cyclic Strain Stimulates Early Growth Response Gene Product 1–Mediated Expression of Membrane Type 1 Matrix Metalloproteinase in Endothelium. Laboratory Investigation 2002, 82: 949-956. PMID: 12118097, DOI: 10.1097/01.lab.0000020408.77307.e9.Peer-Reviewed Original Research
1998
Strain-induced dual alignment of L6 rat skeletal muscle cells
Segurola R, Sumpio B, Mills I. Strain-induced dual alignment of L6 rat skeletal muscle cells. In Vitro Cellular & Developmental Biology - Animal 1998, 34: 609-612. PMID: 9769140, DOI: 10.1007/s11626-996-0004-z.Peer-Reviewed Original Research
1994
Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells
Sumpio B, Widmann M, Ricotta J, Awolesi M, Watase M. Increased ambient pressure stimulates proliferation and morphologic changes in cultured endothelial cells. Journal Of Cellular Physiology 1994, 158: 133-139. PMID: 8263020, DOI: 10.1002/jcp.1041580117.Peer-Reviewed Original ResearchConceptsSystemic blood pressureCultured endothelial cellsBlood pressureSystemic pressureCell responsesEndothelial cell responsesBovine aortic ECsMorphologic changesEndothelial cellsAortic ECsRat type IEC phenotypeSignificant increaseCell numberEC proliferationVascular systemSignificant regulatory influenceCalf serumEndotheliumType IDigital monitorRegulatory influenceProliferationHg pressureCells
1991
Nephrotoxic potential of FK 506.
Sumpio BE, Phan S. Nephrotoxic potential of FK 506. Transplantation Proceedings 1991, 23: 2789-90. PMID: 1721278.Peer-Reviewed Original ResearchAmelioration of renal ischemic injury by phosphocreatine
Rodriguez R, Stepke M, Maitz S, Cuono C, Sumpio B. Amelioration of renal ischemic injury by phosphocreatine. Journal Of Surgical Research 1991, 51: 271-274. PMID: 1921364, DOI: 10.1016/0022-4804(91)90106-v.Peer-Reviewed Original ResearchConceptsRenal ischemic injuryInitial ischemic insultPerfusion of kidneysKrebs-bicarbonate solutionMin of normothermicMM phosphocreatineRenal functionIschemic injuryKidney functionIschemic insultRenal arteryVivo ischemiaWarm ischemiaKrebs solutionKidneyPhosphocreatineAddition of phosphocreatineIschemiaSignificant improvementRegeneration of ATPGFRArteryNormothermicUreterInjury
1989
The influence of perfusate viscosity, RBC deformability and drag on the function of an isolated perfused rat kidney
Sumpio B, Upchurch G, Johnson G. The influence of perfusate viscosity, RBC deformability and drag on the function of an isolated perfused rat kidney. Journal Of Surgical Research 1989, 46: 4-8. PMID: 2915534, DOI: 10.1016/0022-4804(89)90174-1.Peer-Reviewed Original ResearchConceptsRenal functionMean renal arterial pressureRat kidneyKrebs-HCO3 bufferRenal arterial pressureReabsorption of sodiumArterial pressureUrine flowOrgan functionClinical interestHCO3- bufferPerfusate solutionHematocritBlood rheologyFunctional parametersPerfusate viscosityRBC deformabilityKidneyPerfusateDegrees CRBCsDrag-reducing agentsGFRCreatinineAgents
1988
Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro.
Buckley MJ, Banes AJ, Levin LG, Sumpio BE, Sato M, Jordan R, Gilbert J, Link GW, Tran Son Tay R. Osteoblasts increase their rate of division and align in response to cyclic, mechanical tension in vitro. Bone And Mineral 1988, 4: 225-36. PMID: 2847838.Peer-Reviewed Original ResearchCyclosporine toxicity in the isolated perfused rat kidney.
Sumpio BE. Cyclosporine toxicity in the isolated perfused rat kidney. Transplantation Proceedings 1988, 20: 712-6. PMID: 3388509.Peer-Reviewed Original Research
1987
Mitochondrial Respiratory and Calcium Studies
SUMPIO B, BAUE A, CHAUDRY I. Mitochondrial Respiratory and Calcium Studies. Annals Of Surgery 1987, 206: 655-660. PMID: 3499878, PMCID: PMC1493310, DOI: 10.1097/00000658-198711000-00017.Peer-Reviewed Original ResearchConceptsRespiratory control ratioVerapamil pretreatmentTotal tissue Ca2Tissue ATP levelsCyA-induced nephrotoxicityCyA treatmentPharmacologic regimenRenal functionMitochondrial respiratory functionSimultaneous administrationCalcium studiesRespiratory functionKrebs bufferCortical mitochondriaMumol/Tissue Ca2Rat kidneyADP/O ratioCyANephrotoxicityATP levelsControl ratioVerapamilMitochondrial respiratoryCa2Treatment with verapamil and adenosine triphosphate-MgCl2 reduces cyclosporine nephrotoxicity.
Sumpio B, Baue A, Chaudry I. Treatment with verapamil and adenosine triphosphate-MgCl2 reduces cyclosporine nephrotoxicity. Surgery 1987, 101: 315-22. PMID: 2950608.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateRenal perfusate flowTubular absorptionPerfusate flowUrine outputImmunosuppressive agentsVerapamil pretreatmentClearance periodMicrograms/ATP-MgCl2 treatmentControl clearance periodsUse of cyclosporineCombination of verapamilSerious side effectsTissue ATP levelsEffects of adenosineCyA treatmentCyA nephrotoxicityCyclosporine nephrotoxicityVerapamil treatmentTubular functionRenal flowNephrotoxic potentialFiltration rateATP-MgCl2Comparison of effects of ATP-MgCl2 and adenosine-MgCl2 on renal function following ischemia
Sumpio BE, Hull MJ, Baue AE, Chaudry IH. Comparison of effects of ATP-MgCl2 and adenosine-MgCl2 on renal function following ischemia. American Journal Of Physiology 1987, 252: r388-r393. PMID: 3492930, DOI: 10.1152/ajpregu.1987.252.2.r388.Peer-Reviewed Original ResearchConceptsRenal functionATP-MgCl2Warm ischemiaATP-MgCl2 treatmentKrebs-HCO3 bufferMM ATP-MgCl2Comparison of effectsNormothermic ischemiaTransient improvementIschemiaSalutary effectsControl valuesUrine samplesFunctional parametersKidneyMajor breakdown productAdenosineTreatmentBreakdown productsMmHgPerfusateMinAdministration
1986
Effects of ATP-MgCl2 and adenosine-MgCl2 administration on intracellular ATP levels in the kidney
Sumpio B, Hull M, Bauer A, Chaudry I. Effects of ATP-MgCl2 and adenosine-MgCl2 administration on intracellular ATP levels in the kidney. Biochimica Et Biophysica Acta 1986, 862: 303-308. PMID: 3778894, DOI: 10.1016/0005-2736(86)90232-4.Peer-Reviewed Original ResearchConceptsATP-MgCl2 administrationColloid oncotic pressureIntracellular ATPATP-MgCl2Exogenous administrationIntracellular ATP levelsRat kidneyOncotic pressureAdministrationTotal intracellular ATPPerfusate solutionKidneyIntracellular accumulationATP levelsAdenine nucleotidesSignificant accumulationATPPerfusate
1985
Renal handling of proteins in normal and disease states.
Sumpio B, Hayslett J. Renal handling of proteins in normal and disease states. QJM 1985, 57: 611-35. PMID: 4080952.Peer-Reviewed Original ResearchReduction of the drug-induced nephrotoxicity by ATP-MgCl2. 1. Effects on the cis-Diamminedichloroplatinum-treated isolated perfused kidneys
Sumpio B, Chaudry I, Baue A. Reduction of the drug-induced nephrotoxicity by ATP-MgCl2. 1. Effects on the cis-Diamminedichloroplatinum-treated isolated perfused kidneys. Journal Of Surgical Research 1985, 38: 429-437. PMID: 3872963, DOI: 10.1016/0022-4804(85)90058-7.Peer-Reviewed Original ResearchConceptsAcute renal failureATP-MgCl2Hr of perfusionCis-DiamminedichloroplatinumNephrotoxic effectsReabsorptive capacityCDDP treatmentRat kidneyATP-MgCl2 treatmentIsolated rat kidneyDrug-induced nephrotoxicityDirect nephrotoxic effectNew therapeutic modalitiesProximal tubule cellsCancer chemotherapeutic agentsCDDP therapyRenal failureMarked diuresisProtein reabsorptionTherapeutic modalitiesTubule cellsChemotherapeutic agentsMicrograms/KidneyMarked inhibitionReduction of the drug-induced nephrotoxicity by ATP-MgCl2. II. Effects on gentamicin-treated isolated perfused kidneys
Sumpio B, Chaudry I, Baue A. Reduction of the drug-induced nephrotoxicity by ATP-MgCl2. II. Effects on gentamicin-treated isolated perfused kidneys. Journal Of Surgical Research 1985, 38: 438-445. PMID: 3872964, DOI: 10.1016/0022-4804(85)90059-9.Peer-Reviewed Original ResearchConceptsDrug-induced nephrotoxicityGlomerular filtration rateRenal perfusate flowATP-MgCl2Filtration ratePerfusate flowMM ATP-MgCl2Prevention of nephrotoxicityGentamicin-induced nephrotoxicitySignificant clinical problemNon-pulsatile perfusionIsolated kidney preparationMin of perfusionHr of perfusionDose-dependent mannerTubular absorptionUrinary flowGentamicin treatmentProtein reabsorptionClinical problemNephrotoxicityPerfusate concentrationFractional clearanceClearance periodSodium absorptionAdenosine Triphosphate—Magnesium Chloride Ameliorates Reperfusion Injury Following Ischemia as Determined by Phosphorus Nuclear Magnetic Resonance
Sumpio B, Chaudry I, Baue A. Adenosine Triphosphate—Magnesium Chloride Ameliorates Reperfusion Injury Following Ischemia as Determined by Phosphorus Nuclear Magnetic Resonance. JAMA Surgery 1985, 120: 233-240. PMID: 3872113, DOI: 10.1001/archsurg.1985.01390260091013.Peer-Reviewed Original ResearchConceptsIntracellular acidosisPerfusate flowATP levelsAdenosine triphosphate-magnesium chlorideRenal ATP levelsMinutes of reperfusionTissue ATP levelsEffects of ischemiaAdenosine triphosphateML/minPostischemic administrationReperfusion injuryRenal ischemiaWarm ischemiaATP-MgCl2ReperfusionIschemiaInorganic phosphate peakIntracellular adenosine triphosphatePi peakRat kidneyAcidosisATP depletionMinutesMagnetic resonance