2006
Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1
Ueno H, Pradhan S, Schlessel D, Hirasawa H, Sumpio BE. Nicotine enhances human vascular endothelial cell expression of ICAM-1 and VCAM-1 via protein kinase C, p38 mitogen-activated protein kinase, NF-κB, and AP-1. Cardiovascular Toxicology 2006, 6: 39-50. PMID: 16845181, DOI: 10.1385/ct:6:1:39.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsICAM-1VCAM-1NF-κBAdhesion moleculesP38 mitogen-activated protein kinaseProtein kinase CMitogen-activated protein kinaseVascular cell adhesion moleculeEndothelial cellsExposure of HUVECsCellular adhesion molecule expressionAP-1Vascular endothelial cell expressionMajor risk factorEffects of nicotineEtiology of atherosclerosisP38 MAPKAdhesion molecule expressionIntercellular adhesion moleculeTranscription factor NF-κBCellular adhesion moleculesEndothelial cell expressionFactor NF-κBUmbilical vein endothelial cells
2000
Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain
Kito H, Chen E, Wang X, Ikeda M, Azuma N, Nakajima N, Gahtan V, Sumpio B. Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain. Journal Of Applied Physiology 2000, 89: 2391-2400. PMID: 11090594, DOI: 10.1152/jappl.2000.89.6.2391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsFlavonoidsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphorylationPromoter Regions, GeneticPulmonary ArteryPyridinesResponse ElementsStress, MechanicalTetradecanoylphorbol AcetateTranscription Factor AP-1ConceptsPD 98059Activator protein-1SB 203580Protein kinaseBovine pulmonary arterial endothelial cellsPulmonary arterial endothelial cellsArterial endothelial cellsMAPK kinase kinase-1Mitogen-activated protein kinase activationMitogen-activated protein kinaseExtracellular signal-regulated kinaseTerminal protein kinaseKinase kinase 1AP-1/Cell alignmentERK kinase inhibitorProtein kinase activationSignal-regulated kinaseEndothelial cellsTranscriptional activationInactive mutantActivated MAPKsKinase activationKinase 1Transient transfection
1999
Distinct roles for the small GTPases Cdc42 and Rho in endothelial responses to shear stress
Li S, Chen B, Azuma N, Hu Y, Wu S, Sumpio B, Shyy J, Chien S. Distinct roles for the small GTPases Cdc42 and Rho in endothelial responses to shear stress. Journal Of Clinical Investigation 1999, 103: 1141-1150. PMID: 10207166, PMCID: PMC408275, DOI: 10.1172/jci5367.Peer-Reviewed Original ResearchAnimalsBiological TransportCalcium-Calmodulin-Dependent Protein KinasesCattleCdc42 GTP-Binding ProteinCell Cycle ProteinsCells, CulturedCytoskeletonEndothelium, VascularGTP PhosphohydrolasesGTP-Binding ProteinsIntracellular Signaling Peptides and ProteinsJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesPhysical StimulationProtein Serine-Threonine KinasesResponse ElementsRho GTP-Binding ProteinsRho-Associated KinasesRhoA GTP-Binding ProteinTranscription Factor AP-1
1998
Antiproliferative effect of elevated glucose in human microvascular endothelial cells
Kamal K, Du W, Mills I, Sumpio B. Antiproliferative effect of elevated glucose in human microvascular endothelial cells. Journal Of Cellular Biochemistry 1998, 71: 491-501. PMID: 9827695, DOI: 10.1002/(sici)1097-4644(19981215)71:4<491::aid-jcb4>3.0.co;2-p.Peer-Reviewed Original ResearchConceptsMicrovascular endothelial cellsElevated glucoseHMEC-1Endothelial cellsAntiproliferative effectsDiabetic microangiopathyDiabetic foot ulcerationHuman dermal microvascular endothelial cellsCyclic AMP accumulationMicrovascular endothelial cell lineDermal microvascular endothelial cellsHuman microvascular endothelial cellsImmortalized human dermal microvascular endothelial cell lineHigh D-glucoseDeleterious effectsHuman dermal microvascular endothelial cell linePrimary cultured cellsEndothelial cell lineFoot ulcerationHMEC-1 proliferationProliferative responseAMP accumulationPathological complicationsMolecular pathophysiologyRp-cAMPS
1995
Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity
Du W, Mills I, Sumpio B. Cyclic strain causes heterogeneous induction of transcription factors, AP-1, CRE binding protein and NF-kB, in endothelial cells: Species and vascular bed diversity. Journal Of Biomechanics 1995, 28: 1485-1491. PMID: 8666588, DOI: 10.1016/0021-9290(95)00096-8.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 2AnimalsAortaCattleCell DivisionCyclic AMP Response Element-Binding ProteinEndothelium, VascularEpoprostenolGene Expression RegulationGenes, Immediate-EarlyHumansNF-kappa BNuclear ProteinsPhenotypeProtein Kinase CSpecies SpecificityStress, MechanicalTime FactorsTranscription Factor AP-1Transcription FactorsUmbilical VeinsConceptsHuman umbilical vein endothelial cellsBovine aortic endothelial cellsActivator protein-1NF-kBEndothelial cellsVascular bedDifferent vascular bedsUmbilical vein endothelial cellsAortic endothelial cellsCultured endothelial cellsTranscription factor activator protein-1Vein endothelial cellsProstacyclin secretionStretch-induced activationImmediate early genesEC exposureNuclear factorTime pointsHeterogeneous inductionCAMP response elementProtein 1Biphasic mannerSignificant inductionDivergent effectsHuman aorta