1999
Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent
Gahtan V, Wang X, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent. Surgery 1999, 126: 203-207. PMID: 10455885, DOI: 10.1016/s0039-6060(99)70156-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCattleCell Adhesion MoleculesCells, CulturedChemotaxisDose-Response Relationship, DrugFocal Adhesion Protein-Tyrosine KinasesMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularProtein-Tyrosine KinasesThrombospondin 1ConceptsSmooth muscle cell chemotaxisTSP-1-induced chemotaxisThrombospondin-1Protein kinase familyExtracellular signal-regulated proteinExtracellular signal-regulated protein kinase 1/2Vascular smooth muscle cell chemotaxisProtein kinase 1/2Extracellular matrix proteinsCell chemotaxisSerum-free mediumPlatelet-derived growth factorKinase familyKinase 1/2Top chambersFunctional importanceMatrix proteinsERK1/2 activationWestern blot analysisERK1/2 inhibitorBlot analysisERK1/2VSMCPD98059VSMCsThrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells
Gahtan V, Wang X, Ikeda M, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells. Journal Of Vascular Surgery 1999, 29: 1031-1036. PMID: 10359937, DOI: 10.1016/s0741-5214(99)70244-2.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylationAntiphosphotyrosine immunoblottingThrombospondin-1TSP-1-induced VSMC migrationFocal adhesion kinase (FAK) proteinSignal transduction pathwaysFocal adhesion kinaseFocal adhesion plaquesVSMC migrationExtracellular matrix proteinsPlatelet-derived growth factorCrude cell lysatesMolecular weight 68Adhesion kinaseTransduction pathwaysKinase proteinAdhesion plaquesVascular smooth muscle cell proliferationVascular smooth muscle cellsMatrix proteinsCell lysatesPhosphorylationSmooth muscle cell proliferationFAK antibodyProtein bands
1997
Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrinsGene Regulation by Mechanical Forces
Oluwole BO, Du W, Mills I, Sumpio BE. Gene Regulation by Mechanical Forces. Endothelium 1997, 5: 85-93. PMID: 9237042, DOI: 10.3109/10623329709079866.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell Adhesion MoleculesCell Culture TechniquesCells, CulturedEndothelinsEndothelium, VascularEpoprostenolFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression RegulationHemorheologyHumansMechanoreceptorsMembranes, ArtificialNitric OxideProtein-Tyrosine KinasesSignal TransductionStress, MechanicalTissue Plasminogen ActivatorTranscription FactorsTranscription, GeneticVacuumConceptsEndothelial cellsEndothelial cell functionCyclic strainVasoactive mediatorsSecond messenger cascadesNitric oxideBlood vesselsMechanical forcesCell functionMechanical deformationFlow of bloodMessenger cascadesLuminal surfaceSpecific response elementsPromoter geneResponse elementExternal forcesMediatorsCytoskeletal changesCellsForceProstacyclinExpressionTranscription levels
1996
Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostinsMatrix-specific effect of endothelial control of smooth muscle cell migration
Powell R, Carruth J, Basson M, Bloodgood R, Sumpio B, Surgery Y. Matrix-specific effect of endothelial control of smooth muscle cell migration. Journal Of Vascular Surgery 1996, 24: 51-57. PMID: 8691527, DOI: 10.1016/s0741-5214(96)70144-1.Peer-Reviewed Original ResearchInvolvement of rho p21 in Cyclic Strain-Induced Tyrosine Phosphorylation of Focal Adhesion Kinase (pp125FAK), Morphological Changes and Migration of Endothelial Cells
Yano Y, Saito Y, Narumiya S, Sumpio B. Involvement of rho p21 in Cyclic Strain-Induced Tyrosine Phosphorylation of Focal Adhesion Kinase (pp125FAK), Morphological Changes and Migration of Endothelial Cells. Biochemical And Biophysical Research Communications 1996, 224: 508-515. PMID: 8702419, DOI: 10.1006/bbrc.1996.1057.Peer-Reviewed Original ResearchAdenosine Diphosphate RiboseADP Ribose TransferasesAnimalsAorta, ThoracicBotulinum ToxinsCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGTP-Binding ProteinsMitomycinsPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesRho GTP-Binding ProteinsStress, Mechanical