2005
The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain
Kakisis JD, Pradhan S, Cordova A, Liapis CD, Sumpio BE. The role of STAT-3 in the mediation of smooth muscle cell response to cyclic strain. The International Journal Of Biochemistry & Cell Biology 2005, 37: 1396-1406. PMID: 15833272, DOI: 10.1016/j.biocel.2005.01.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCell ProliferationCells, CulturedDNA-Binding ProteinsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myocytes, Smooth MusclePhosphorylationPyrazolesPyrimidinesRatsSerineSignal TransductionSrc-Family KinasesSTAT3 Transcription FactorStress, MechanicalTrans-ActivatorsTyrosineConceptsSerine phosphorylationTyrosine phosphorylationSmooth muscle cellsSTAT-3Vascular smooth muscle cellsSTAT-3 tyrosine phosphorylationSpecific inhibitorVascular cell morphologyExtracellular signal-regulated kinase 1/2Extracellular mechanical signalsSignal-regulated kinase 1/2Basal serine phosphorylationInhibition of SrcA7r5 smooth muscle cellsNegative feedback loopInhibition of ERK1/2Cells sensePhosphospecific antibodiesPhosphatidylinositol 3Signal transducerTranscription 3Kinase 2Kinase 1/2Smooth muscle cell responseBasal phosphorylation
2000
Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes
Fujioka K, Azuma N, Kito H, Gahtan V, Esato K, Sumpio B. Role of Caveolin in Hemodynamic Force-Mediated Endothelial Changes. Journal Of Surgical Research 2000, 92: 7-10. PMID: 10864474, DOI: 10.1006/jsre.2000.5838.Peer-Reviewed Original ResearchEffects of increased ambient pressure on colon cancer cell adhesion
Basson M, Yu C, Herden‐Kirchoff O, Ellermeier M, Sanders M, Merrell R, Sumpio B. Effects of increased ambient pressure on colon cancer cell adhesion. Journal Of Cellular Biochemistry 2000, 78: 47-61. PMID: 10797565, DOI: 10.1002/(sici)1097-4644(20000701)78:1<47::aid-jcb5>3.0.co;2-m.Peer-Reviewed Original ResearchConceptsColon cancer cell adhesionCancer cell adhesionTyrosine phosphorylationCell adhesionPressure-stimulated adhesionFocal adhesion kinaseHuman colon cancer cellsIntestinal epithelial biologyColon cancer cellsColon cancer cell linesHuman colon cancer cell linesBeta1 integrin subunitsHuman colon cancerAdhesion kinaseBovine aortic smooth muscle cellsAortic smooth muscle cellsIntracellular proteinsEpithelial biologyMatrix proteinsAbsence of serumIntegrin subunitsCancer cell linesPrimary cellsSmooth muscle cellsKinase inhibition
1999
Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells
Gahtan V, Wang X, Ikeda M, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells. Journal Of Vascular Surgery 1999, 29: 1031-1036. PMID: 10359937, DOI: 10.1016/s0741-5214(99)70244-2.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylationAntiphosphotyrosine immunoblottingThrombospondin-1TSP-1-induced VSMC migrationFocal adhesion kinase (FAK) proteinSignal transduction pathwaysFocal adhesion kinaseFocal adhesion plaquesVSMC migrationExtracellular matrix proteinsPlatelet-derived growth factorCrude cell lysatesMolecular weight 68Adhesion kinaseTransduction pathwaysKinase proteinAdhesion plaquesVascular smooth muscle cell proliferationVascular smooth muscle cellsMatrix proteinsCell lysatesPhosphorylationSmooth muscle cell proliferationFAK antibodyProtein bands
1998
Mechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells
Han O, Sumpio B, Basson M. Mechanical Strain Rapidly Redistributes Tyrosine Phosphorylated Proteins in Human Intestinal Caco-2 Cells. Biochemical And Biophysical Research Communications 1998, 250: 668-673. PMID: 9784404, DOI: 10.1006/bbrc.1998.9372.Peer-Reviewed Original ResearchConceptsCaco-2 cellsTyrosine kinase activityHuman intestinal Caco-2 cellsIntestinal Caco-2 cellsCaco-2 intestinal cellsRepetitive strainHuman Caco-2 intestinal cellsCaco-2 intestinal epithelial cellsIntestinal epithelial cellsHuman Caco-2 intestinal epithelial cellsKinase activityTyrosine phosphorylationIntestinal cellsEpithelial cellsModulates differentiationParticulate fractionTyrosine phosphorylated proteinsCellsSubcellular distribution
1997
Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells
Yano Y, Geibel J, Sumpio B. Cyclic strain induces reorganization of integrin α5β1 and α2β1 in human umbilical vein endothelial cells. Journal Of Cellular Biochemistry 1997, 64: 505-513. PMID: 9057108, DOI: 10.1002/(sici)1097-4644(19970301)64:3<505::aid-jcb17>3.0.co;2-e.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsBeta 1 integrinUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsAlpha 5Beta 1Alpha 5 beta 1Alpha 2 beta 1 integrinH exposureBeta 3 integrinAlpha 2 integrinCycles/minEndothelial cell morphologyVariety of stimuliIntegrin alpha 5Tyrosine phosphorylationVitronectin receptorAlpha 2Redistribution of alphaEC subjectsBeta integrins
1996
Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain
Yano Y, Geibel J, Sumpio BE. Tyrosine phosphorylation of pp125FAK and paxillin in aortic endothelial cells induced by mechanical strain. American Journal Of Physiology 1996, 271: c635-c649. PMID: 8770005, DOI: 10.1152/ajpcell.1996.271.2.c635.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell Adhesion MoleculesCell MovementCells, CulturedCytoskeletal ProteinsEndothelium, VascularFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGenisteinIsoflavonesNitrilesPaxillinPhosphoproteinsPhosphorylationPhosphotyrosineProtein-Tyrosine KinasesStress, MechanicalTissue DistributionTyrosineTyrphostins