2010
Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis
Willis AI, Sadowitz B, Fuse S, Maier KG, Lee TS, Wang XJ, Tuszynski GP, Sumpio BE, Gahtan V. Thrombospondin 1, Fibronectin, and Vitronectin are Differentially Dependent Upon RAS, ERK1/2, and p38 for Induction of Vascular Smooth Muscle Cell Chemotaxis. Vascular And Endovascular Surgery 2010, 45: 55-62. PMID: 21193465, DOI: 10.1177/1538574410387677.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsCattleCells, CulturedChemotaxisFibronectinsHumansMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Muscle, Smooth, VascularMyocytes, Smooth MuscleP38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsRas ProteinsThrombospondin 1TransfectionVitronectinConceptsVascular smooth muscle cellsThrombospondin-1Smooth muscle cell chemotaxisFarnesyl protein transferase inhibitorSignal transduction pathwaysProtein transferase inhibitorsVascular smooth muscle cell chemotaxisBovine vascular smooth muscle cellsTSP-1Ras N17Transduction pathwaysSecond messenger systemsP38Smooth muscle cellsERK1/2VSMC migrationTransferase inhibitorsPD098059Cell chemotaxisSB202190Muscle cellsMessenger systemsVitronectinRAChemotaxis
2009
Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1
Esemuede N, Lee T, Maier KG, Sumpio BE, Gahtan V. Lovastatin Inhibits Thrombospondin-1-Induced Smooth Muscle Cell Chemotaxis1. Journal Of Surgical Research 2009, 168: 149-154. PMID: 20338582, DOI: 10.1016/j.jss.2009.11.728.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell ProliferationCells, CulturedChemotaxisDose-Response Relationship, DrugHydroxymethylglutaryl-CoA Reductase InhibitorsLovastatinMevalonic AcidModels, AnimalMonomeric GTP-Binding ProteinsMuscle, Smooth, VascularRas ProteinsSignal TransductionThrombospondin 1Tunica IntimaTunica MediaConceptsTSP-1-induced chemotaxisGeranylgeranyl transferase inhibitorSerum-free mediumThrombospondin-1HMG-CoA reductase inhibitorsVascular smooth muscle cell migrationSmooth muscle cell migrationTransferase inhibitorsEffect of lovastatinG proteinsMuscle cell migrationRas activationRho-kinase inhibitorCell migrationPost-hoc testingFarnesyl transferase inhibitorsCholesterol loweringLovastatin doseVascular restenosisIntimal hyperplasiaBoyden chamberPleiotropic propertiesReductase inhibitorsWestern blotInhibition of Ras
2008
The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration
Fuse S, Esemuede N, Yamaguchi M, Maier KG, Nesselroth SM, Sumpio BE, Gahtan V. The role of G proteins in thromospondin-1–induced vascular smooth muscle cell migration. Surgery 2008, 144: 86-92. PMID: 18571589, DOI: 10.1016/j.surg.2008.03.028.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsTSP-1-induced chemotaxisExtracellular signal-regulated kinase 1/2Pertussis toxinSerum-free mediumCholera toxinThrombospondin-1CAMP levelsP38 activationG proteinsVascular smooth muscle cell migrationQuiescent vascular smooth muscle cellsSmooth muscle cell migrationCyclic adenosine monophosphate levelsSmooth muscle cellsIntracellular cyclic adenosine monophosphate (cAMP) levelsMuscle cell migrationTSP-1 receptorAdenosine monophosphate levelsSignal-regulated kinase 1/2Levels of cAMPArterial lesionsVascular injuryVSMC chemotaxisMicrochemotaxis chamberThrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase
Wang XJ, Maier K, Fuse S, Willis AI, Olson E, Nesselroth S, Sumpio BE, Gahtan V. Thrombospondin-1-Induced Migration Is Functionally Dependent Upon Focal Adhesion Kinase. Vascular And Endovascular Surgery 2008, 42: 256-262. PMID: 18319354, DOI: 10.1177/1538574408314440.Peer-Reviewed Original Research
2004
The role of thrombospondin-1 in human disease1
Esemuede N, Lee T, Pierre-Paul D, Sumpio BE, Gahtan V. The role of thrombospondin-1 in human disease1. Journal Of Surgical Research 2004, 122: 135-142. PMID: 15522326, DOI: 10.1016/j.jss.2004.05.015.Peer-Reviewed Original Research
2003
Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none.
Lee T, Esemuede N, Sumpio BE, Gahtan V. Thrombospondin-1 induces matrix metalloproteinase-2 activation in vascular smooth muscle cells1 1Competition of interest: none. Journal Of Vascular Surgery 2003, 38: 147-154. PMID: 12844104, DOI: 10.1016/s0741-5214(02)75468-2.Peer-Reviewed Original ResearchConceptsThrombospondin-1TSP-1-induced VSMC migrationVSMC migrationCell migrationMatrix metalloproteinase-2MMP2 activationMatrix barrierSerum-free mediumNorthern blot analysisVascular smooth muscle cell migrationCellular processesExtracellular matrix glycoproteinMMP2 activityPosttranslational mechanismsMatrix metalloproteinase-2 activationMetalloproteinase-2 activationSmooth muscle cell migrationMuscle cell migrationInvasion assaysStrong binding activityBinding activityECM degradationMatrix glycoproteinRNA expressionBlot analysisThrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains
Lee T, Nesselroth SM, Olson ET, Esemuede N, Lawler J, Sumpio BE, Gahtan V. Thrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains. Journal Of Cellular Biochemistry 2003, 89: 500-506. PMID: 12761883, DOI: 10.1002/jcb.10524.Peer-Reviewed Original ResearchConceptsVSMC chemotaxisThrombospondin-1Acute phase reactantsSmooth muscle cell chemotaxisVascular diseaseVascular smooth muscle cell chemotaxisPhase reactantsExtracellular signal-regulated kinases 1Microchemotaxis chamberSignal-regulated kinases 1Serum-free mediumCell chemotaxisMatricellular glycoproteinActivate ERKWestern immunoblottingVSMCsT-testP38 activationP38 pathwayERKFusion proteinT3ChemotaxisKinase 1P38 kinase
2001
The C-terminal domain of thrombospondin-1 induces vascular smooth muscle cell chemotaxis
Nesselroth S, Willis A, Fuse S, Olson E, Lawler J, Sumpio B, Gahtan V. The C-terminal domain of thrombospondin-1 induces vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2001, 33: 595-600. PMID: 11241132, DOI: 10.1067/mva.2001.112318.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVSMC chemotaxisThrombospondin-1VSMC migrationAortic vascular smooth muscle cellsTop wellsAcute phase reactantsBoyden chamber chemotaxisSmooth muscle cellsSmooth muscle cell chemotaxisVascular diseaseVascular lesionsVascular smooth muscle cell chemotaxisSerum-free mediumMuscle cellsTSP-1 moleculeCell chemotaxisChemotaxis assaysBottom wellsAntibodiesSelective inhibitionT-test
2000
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis
Willis A, Fuse S, Wang X, Chen E, Tuszynski G, Sumpio B, Gahtan V. Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2000, 31: 1160-1167. PMID: 10842153, DOI: 10.1067/mva.2000.106489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell CountCell DivisionCells, CulturedChemotaxisChromonesEnzyme InhibitorsExtracellular Matrix ProteinsFibronectinsHyperplasiaIndolesMaleimidesMorpholinesMuscle, Smooth, VascularPhosphoinositide-3 Kinase InhibitorsProtein Kinase CSignal TransductionStatistics as TopicThrombospondin 1Tunica IntimaVitronectinConceptsProtein kinase CVSMC migrationLY-294002Thrombospondin-1ECM proteinsKinase CSmooth muscle cell chemotaxisSerum-free mediumCellular signaling pathwaysInhibition of phosphatidylinositolVascular smooth muscle cell chemotaxisVascular smooth muscle cell migrationPI3K inhibitorsSmooth muscle cell migrationSignaling pathwaysMuscle cell migrationBisindolylmaleimide ICell migrationPKC inhibitorGF 109203XExtracellular matrix depositionTop wellsK inhibitorsAortic VSMCsCell chemotaxis
1999
Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent
Gahtan V, Wang X, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 regulation of smooth muscle cell chemotaxis is extracellular signal-regulated protein kinases 1/2 dependent. Surgery 1999, 126: 203-207. PMID: 10455885, DOI: 10.1016/s0039-6060(99)70156-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCattleCell Adhesion MoleculesCells, CulturedChemotaxisDose-Response Relationship, DrugFocal Adhesion Protein-Tyrosine KinasesMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularProtein-Tyrosine KinasesThrombospondin 1ConceptsSmooth muscle cell chemotaxisTSP-1-induced chemotaxisThrombospondin-1Protein kinase familyExtracellular signal-regulated proteinExtracellular signal-regulated protein kinase 1/2Vascular smooth muscle cell chemotaxisProtein kinase 1/2Extracellular matrix proteinsCell chemotaxisSerum-free mediumPlatelet-derived growth factorKinase familyKinase 1/2Top chambersFunctional importanceMatrix proteinsERK1/2 activationWestern blot analysisERK1/2 inhibitorBlot analysisERK1/2VSMCPD98059VSMCsThrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells
Gahtan V, Wang X, Ikeda M, Willis A, Tuszynski G, Sumpio B. Thrombospondin-1 induces activation of focal adhesion kinase in vascular smooth muscle cells. Journal Of Vascular Surgery 1999, 29: 1031-1036. PMID: 10359937, DOI: 10.1016/s0741-5214(99)70244-2.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylationAntiphosphotyrosine immunoblottingThrombospondin-1TSP-1-induced VSMC migrationFocal adhesion kinase (FAK) proteinSignal transduction pathwaysFocal adhesion kinaseFocal adhesion plaquesVSMC migrationExtracellular matrix proteinsPlatelet-derived growth factorCrude cell lysatesMolecular weight 68Adhesion kinaseTransduction pathwaysKinase proteinAdhesion plaquesVascular smooth muscle cell proliferationVascular smooth muscle cellsMatrix proteinsCell lysatesPhosphorylationSmooth muscle cell proliferationFAK antibodyProtein bands