2014
African Trypanosome-Induced Blood–Brain Barrier Dysfunction under Shear Stress May Not Require ERK Activation
Sumpio BJ, Chitragari G, Moriguchi T, Shalaby S, Pappas-Brown V, Khan AM, Sekaran SD, Sumpio BE, Grab DJ. African Trypanosome-Induced Blood–Brain Barrier Dysfunction under Shear Stress May Not Require ERK Activation. International Journal Of Angiology 2014, 24: 41-46. PMID: 27053915, PMCID: PMC4813428, DOI: 10.1055/s-0034-1370890.Peer-Reviewed Original ResearchHuman brain microvascular endothelial cellsBrain microvascular endothelial cellsTransendothelial electrical resistanceBlood-brain barrierBrain postcapillary venulesBrain barrier dysfunctionMicrovascular endothelial cellsPhosphorylation of ERK1/2Parasite cysteine proteasesNeurological symptomsBBB permeabilityBarrier dysfunctionExtracellular signal-regulated kinaseBrain capillariesMicrovascular permeabilityAbsence of trypanosomesHuman African trypanosomiasisSignal-regulated kinasePostcapillary venulesTransient increaseEndothelial cellsBarrier functionElectric cell-substrate impedance sensingProtozoan parasiteTrypanosome infection
2007
Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. Effect of different frequencies of tensile strain on human dermal fibroblast proliferation and survival. Wound Repair And Regeneration 2007, 15: 646-656. PMID: 17971010, DOI: 10.1111/j.1524-475x.2007.00295.x.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMAPK/ERK kinaseExtracellular signal-regulated kinaseDominant-negative AktHuman dermal fibroblastsSignal-regulated kinaseCell numberERK kinaseProtein kinaseTransduction pathwaysDermal fibroblastsRelevant transduction pathwaysRelevant signal pathwaysDermal fibroblast proliferationHuman dermal fibroblast proliferationCultured human dermal fibroblastsSurvival of fibroblastsSignal pathwayKinaseNecrotic fibroblastsDNA synthesisCell proliferationAktTransferase-mediated dUTP nick-end labeling staining
2001
Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells
Li W, Duzgun A, Sumpio B, Basson M. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. AJP Gastrointestinal And Liver Physiology 2001, 280: g75-g87. PMID: 11123200, DOI: 10.1152/ajpgi.2001.280.1.g75.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell DivisionCell MovementCytoskeletal ProteinsEnzyme InhibitorsExtracellular MatrixFlavonoidsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression Regulation, EnzymologicHumansImidazolesIntegrinsIntestinesMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNaphthalenesP38 Mitogen-Activated Protein KinasesPaxillinPhosphoproteinsPhosphorylationProtein-Tyrosine KinasesPyridinesStress, MechanicalTransfectionConceptsFocal adhesion kinaseMAPK extracellular signal-regulated kinaseCaco-2 cellsMitogen-activated protein kinase activationVitro kinase assaysProtein kinase activationSignal-regulated kinaseMitogenic effectProtein kinase C inhibitionBeta1 integrin subunitsCaco-2 proliferationKinase assaysAdhesion kinaseFAK activationERK signalsJNK1 activationKinase activationTerminal kinaseMAPK activationUpstream signalsC-JunFAK inhibitionP38 inhibitionIntegrin subunitsKinase
2000
Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain
Kito H, Chen E, Wang X, Ikeda M, Azuma N, Nakajima N, Gahtan V, Sumpio B. Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain. Journal Of Applied Physiology 2000, 89: 2391-2400. PMID: 11090594, DOI: 10.1152/jappl.2000.89.6.2391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsFlavonoidsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphorylationPromoter Regions, GeneticPulmonary ArteryPyridinesResponse ElementsStress, MechanicalTetradecanoylphorbol AcetateTranscription Factor AP-1ConceptsPD 98059Activator protein-1SB 203580Protein kinaseBovine pulmonary arterial endothelial cellsPulmonary arterial endothelial cellsArterial endothelial cellsMAPK kinase kinase-1Mitogen-activated protein kinase activationMitogen-activated protein kinaseExtracellular signal-regulated kinaseTerminal protein kinaseKinase kinase 1AP-1/Cell alignmentERK kinase inhibitorProtein kinase activationSignal-regulated kinaseEndothelial cellsTranscriptional activationInactive mutantActivated MAPKsKinase activationKinase 1Transient transfection