2000
Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis
Willis A, Fuse S, Wang X, Chen E, Tuszynski G, Sumpio B, Gahtan V. Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis. Journal Of Vascular Surgery 2000, 31: 1160-1167. PMID: 10842153, DOI: 10.1067/mva.2000.106489.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell CountCell DivisionCells, CulturedChemotaxisChromonesEnzyme InhibitorsExtracellular Matrix ProteinsFibronectinsHyperplasiaIndolesMaleimidesMorpholinesMuscle, Smooth, VascularPhosphoinositide-3 Kinase InhibitorsProtein Kinase CSignal TransductionStatistics as TopicThrombospondin 1Tunica IntimaVitronectinConceptsProtein kinase CVSMC migrationLY-294002Thrombospondin-1ECM proteinsKinase CSmooth muscle cell chemotaxisSerum-free mediumCellular signaling pathwaysInhibition of phosphatidylinositolVascular smooth muscle cell chemotaxisVascular smooth muscle cell migrationPI3K inhibitorsSmooth muscle cell migrationSignaling pathwaysMuscle cell migrationBisindolylmaleimide ICell migrationPKC inhibitorGF 109203XExtracellular matrix depositionTop wellsK inhibitorsAortic VSMCsCell chemotaxis
1997
Cyclic strain stimulates isoform‐specific PKC activation and translocation in cultured human keratinocytes
Takei T, Han O, Ikeda M, Male P, Mills I, Sumpio B. Cyclic strain stimulates isoform‐specific PKC activation and translocation in cultured human keratinocytes. Journal Of Cellular Biochemistry 1997, 67: 327-337. PMID: 9361188, DOI: 10.1002/(sici)1097-4644(19971201)67:3<327::aid-jcb5>3.0.co;2-y.Peer-Reviewed Original Research
1992
Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells
Rosales O, Isales C, Nathanson M, Sumpio B. Immunocytochemical expression and localization of protein kinase C in bovine aortic endothelial cells. Biochemical And Biophysical Research Communications 1992, 189: 40-46. PMID: 1449492, DOI: 10.1016/0006-291x(92)91522-r.Peer-Reviewed Original ResearchConceptsSerum-deprived mediumPKC activityPhorbol ester-induced increaseAortic endothelial cellsBovine aortic endothelial cellsBeta stainingSDM groupTotal PKC activityImmunocytochemical expressionBeta PKC isoformsEndothelial cellsParticulate fractionFBS groupProtein kinase CCalphostin CPKC inhibitorPhorbol esterConfocal microscopyDiffuse cytoplasmic localizationCytosolic fractionGamma-PKCKinase CPKC isoformsCytoplasmic localizationCells