2006
p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress
Kadohama T, Akasaka N, Nishimura K, Hoshino Y, Sasajima T, Sumpio BE. p38 Mitogen-Activated Protein Kinase Activation in Endothelial Cell Is Implicated in Cell Alignment and Elongation Induced by Fluid Shear Stress. Endothelium 2006, 13: 43-50. PMID: 16885066, DOI: 10.1080/10623320600660219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAtherosclerosisBlood PressureBlood VesselsCattleCell PolarityCell ShapeCells, CulturedDose-Response Relationship, DrugEndothelial CellsEnzyme ActivationEnzyme InhibitorsHemodynamicsMAP Kinase Signaling SystemP38 Mitogen-Activated Protein KinasesPhosphorylationRegional Blood FlowStress, MechanicalTime FactorsConceptsStatic endothelial cellsFluid shear stressP38 MAPKP38 Mitogen-Activated Protein Kinase ActivationMitogen-Activated Protein Kinase ActivationEndothelial cellsProtein kinase activationP38 MAPK inhibitor SB-203580Inhibitor SB 203580Cell alignmentProtein kinaseBovine aortic endothelial cellsLaminar shear stressKinase activationSB 203580P38 mitogenCultured bovine aortic endothelial cellsEC elongationAortic endothelial cellsMAPKCellsElongationActivationMorphometric analysisKinase
2003
Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling
Yamashita A, Hanna AK, Hirata S, Dardik A, Sumpio BE. Antisense basic fibroblast growth factor alters the time course of mitogen-activated protein kinase in arterialized vein graft remodeling. Journal Of Vascular Surgery 2003, 37: 866-873. PMID: 12663990, DOI: 10.1067/mva.2003.130.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Vessel ProsthesisCell Physiological PhenomenaEndothelium, VascularFibroblast Growth FactorsHyperplasiaJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesModels, AnimalP38 Mitogen-Activated Protein KinasesProteinsRabbitsRegenerationRNA, AntisenseTime FactorsTransfectionVeinsConceptsProtein kinaseBasic fibroblast growth factorMAPK activationFibroblast growth factorMitogen-activated protein kinase (MAPK) familyC-Jun N-terminal protein kinaseProtein kinase familyExtracellular signal-regulated kinase 1/2Antisense basic fibroblast growth factorMitogen-activated protein kinaseSignal-regulated kinase 1/2Messenger RNA sequencesP38 kinase activationGrowth factor altersMAPK-dependent mechanismFibroblast growth factor activityGrowth factorKinase familyKinase activationGrowth factor activityPhosphorylation of MAPKsKinase 1/2RNA sequencesWestern blot analysisBasic fibroblast growth factor activity
2001
Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells
Li W, Duzgun A, Sumpio B, Basson M. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. AJP Gastrointestinal And Liver Physiology 2001, 280: g75-g87. PMID: 11123200, DOI: 10.1152/ajpgi.2001.280.1.g75.Peer-Reviewed Original ResearchMeSH KeywordsCaco-2 CellsCell DivisionCell MovementCytoskeletal ProteinsEnzyme InhibitorsExtracellular MatrixFlavonoidsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene Expression Regulation, EnzymologicHumansImidazolesIntegrinsIntestinesMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein Kinase 8Mitogen-Activated Protein Kinase 9Mitogen-Activated Protein KinasesNaphthalenesP38 Mitogen-Activated Protein KinasesPaxillinPhosphoproteinsPhosphorylationProtein-Tyrosine KinasesPyridinesStress, MechanicalTransfectionConceptsFocal adhesion kinaseMAPK extracellular signal-regulated kinaseCaco-2 cellsMitogen-activated protein kinase activationVitro kinase assaysProtein kinase activationSignal-regulated kinaseMitogenic effectProtein kinase C inhibitionBeta1 integrin subunitsCaco-2 proliferationKinase assaysAdhesion kinaseFAK activationERK signalsJNK1 activationKinase activationTerminal kinaseMAPK activationUpstream signalsC-JunFAK inhibitionP38 inhibitionIntegrin subunitsKinase
2000
Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain
Kito H, Chen E, Wang X, Ikeda M, Azuma N, Nakajima N, Gahtan V, Sumpio B. Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain. Journal Of Applied Physiology 2000, 89: 2391-2400. PMID: 11090594, DOI: 10.1152/jappl.2000.89.6.2391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsFlavonoidsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphorylationPromoter Regions, GeneticPulmonary ArteryPyridinesResponse ElementsStress, MechanicalTetradecanoylphorbol AcetateTranscription Factor AP-1ConceptsPD 98059Activator protein-1SB 203580Protein kinaseBovine pulmonary arterial endothelial cellsPulmonary arterial endothelial cellsArterial endothelial cellsMAPK kinase kinase-1Mitogen-activated protein kinase activationMitogen-activated protein kinaseExtracellular signal-regulated kinaseTerminal protein kinaseKinase kinase 1AP-1/Cell alignmentERK kinase inhibitorProtein kinase activationSignal-regulated kinaseEndothelial cellsTranscriptional activationInactive mutantActivated MAPKsKinase activationKinase 1Transient transfectionHomocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive form
1999
Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain
Ikeda M, Kito H, Sumpio B. Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain. Biochemical And Biophysical Research Communications 1999, 257: 668-671. PMID: 10208841, DOI: 10.1006/bbrc.1999.0532.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsAortaCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChromonesEndothelium, VascularEnzyme ActivationGuanosine TriphosphateHemodynamicsMorpholinesOncogene Protein p21(ras)Phosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationSignal TransductionStress, MechanicalTime FactorsWortmanninConceptsERK1/2 activationPhosphatidylinositol-3 kinase inhibitorMAP kinase activationExtracellular signal-regulated protein kinases 1Endothelial cellsMechanical stretchKinase inhibitorsERK1/2 phosphorylationEffect of wortmanninPossible involvementHemodynamic forcesProtein kinase 1ActivationKinase 1Upstream pathwaysRapid activationKinase activationSignal transduction pathwaysP21ras activationLY294002ERK1 activationERK2 activation