2003
Thrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains
Lee T, Nesselroth SM, Olson ET, Esemuede N, Lawler J, Sumpio BE, Gahtan V. Thrombospondin‐1‐induced vascular smooth muscle cell chemotaxis: The role of the type 3 repeat and carboxyl terminal domains. Journal Of Cellular Biochemistry 2003, 89: 500-506. PMID: 12761883, DOI: 10.1002/jcb.10524.Peer-Reviewed Original ResearchConceptsVSMC chemotaxisThrombospondin-1Acute phase reactantsSmooth muscle cell chemotaxisVascular diseaseVascular smooth muscle cell chemotaxisPhase reactantsExtracellular signal-regulated kinases 1Microchemotaxis chamberSignal-regulated kinases 1Serum-free mediumCell chemotaxisMatricellular glycoproteinActivate ERKWestern immunoblottingVSMCsT-testP38 activationP38 pathwayERKFusion proteinT3ChemotaxisKinase 1P38 kinase
2000
Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain
Kito H, Chen E, Wang X, Ikeda M, Azuma N, Nakajima N, Gahtan V, Sumpio B. Role of mitogen-activated protein kinases in pulmonary endothelial cells exposed to cyclic strain. Journal Of Applied Physiology 2000, 89: 2391-2400. PMID: 11090594, DOI: 10.1152/jappl.2000.89.6.2391.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCattleCell MovementCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsFlavonoidsImidazolesJNK Mitogen-Activated Protein KinasesMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphorylationPromoter Regions, GeneticPulmonary ArteryPyridinesResponse ElementsStress, MechanicalTetradecanoylphorbol AcetateTranscription Factor AP-1ConceptsPD 98059Activator protein-1SB 203580Protein kinaseBovine pulmonary arterial endothelial cellsPulmonary arterial endothelial cellsArterial endothelial cellsMAPK kinase kinase-1Mitogen-activated protein kinase activationMitogen-activated protein kinaseExtracellular signal-regulated kinaseTerminal protein kinaseKinase kinase 1AP-1/Cell alignmentERK kinase inhibitorProtein kinase activationSignal-regulated kinaseEndothelial cellsTranscriptional activationInactive mutantActivated MAPKsKinase activationKinase 1Transient transfectionHomocysteine stimulates MAP kinase in bovine aortic smooth muscle cells
Woo D, Dudrick S, Sumpio B. Homocysteine stimulates MAP kinase in bovine aortic smooth muscle cells. Surgery 2000, 128: 59-66. PMID: 10876187, DOI: 10.1067/msy.2000.106531.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell DivisionCells, CulturedDose-Response Relationship, DrugEnzyme ActivationEnzyme InhibitorsFlavonoidsHomocysteineMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesMuscle, Smooth, VascularPhosphorylationConceptsMAP kinase phosphorylationMAP kinaseKinase phosphorylationBASMC proliferationBovine aortic smooth muscle cellsAortic smooth muscle cellsCell proliferationExtracellular signal-regulated protein kinases 1Mitogen-activated protein kinaseSmooth muscle cell proliferationProtein kinase 1Smooth muscle cellsMuscle cell proliferationMAP kinase activationDegree of phosphorylationL-homocysteineMuscle cellsProtein kinaseKinase studiesKinase activationKinase 1Hallmark of atherosclerosisKinasePhosphorylationActive form
1999
Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain
Ikeda M, Kito H, Sumpio B. Phosphatidylinositol-3 Kinase Dependent MAP Kinase Activation via p21ras in Endothelial Cells Exposed to Cyclic Strain. Biochemical And Biophysical Research Communications 1999, 257: 668-671. PMID: 10208841, DOI: 10.1006/bbrc.1999.0532.Peer-Reviewed Original ResearchMeSH KeywordsAndrostadienesAnimalsAortaCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChromonesEndothelium, VascularEnzyme ActivationGuanosine TriphosphateHemodynamicsMorpholinesOncogene Protein p21(ras)Phosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationSignal TransductionStress, MechanicalTime FactorsWortmanninConceptsERK1/2 activationPhosphatidylinositol-3 kinase inhibitorMAP kinase activationExtracellular signal-regulated protein kinases 1Endothelial cellsMechanical stretchKinase inhibitorsERK1/2 phosphorylationEffect of wortmanninPossible involvementHemodynamic forcesProtein kinase 1ActivationKinase 1Upstream pathwaysRapid activationKinase activationSignal transduction pathwaysP21ras activationLY294002ERK1 activationERK2 activationExtracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain
Ikeda M, Takei T, Mills I, Kito H, Sumpio B. Extracellular signal-regulated kinases 1 and 2 activation in endothelial cells exposed to cyclic strain. American Journal Of Physiology 1999, 276: h614-h622. PMID: 9950863, DOI: 10.1152/ajpheart.1999.276.2.h614.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesCattleCell DivisionCells, CulturedEndothelium, VascularEnzyme ActivationEnzyme InhibitorsExtracellular SpaceFlavonoidsIntracellular MembranesPhosphorylationProtein Kinase CProtein-Tyrosine KinasesStress, MechanicalConceptsERK1/ERK2Bovine aortic endothelial cellsExtracellular signal-regulated kinases 1ERK1/ERK2 activationExtracellular signal-regulated kinases 1/2Signal-regulated kinases 1/2Tyrosine kinase inhibitor genisteinCell proliferationTreatment of BAECProtein kinase C activationProtein kinase C inhibitor calphostin CTime-dependent phosphorylationKinase inhibitor genisteinKinase C activationERK2 activationEndothelial cellsERK2Strain-induced activationKinase 1Kinase 1/2PD 98059Tyrosine kinaseInhibitor genisteinCalphostin CStrain-dependent activation
1998
Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain
Ikeda M, Takei T, Mills I, Sumpio B. Calcium-Independent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Cyclic Strain. Biochemical And Biophysical Research Communications 1998, 247: 462-465. PMID: 9642151, DOI: 10.1006/bbrc.1998.8811.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCalcium Channel BlockersCalcium-Calmodulin-Dependent Protein KinasesCattleCells, CulturedChelating AgentsEgtazic AcidEndothelium, VascularEnzyme ActivationExtracellular SpaceGadoliniumHydroquinonesIntracellular FluidMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalConceptsEndothelial cellsActivation of ERK1/2Baseline phosphorylationExtracellular Ca2Normal extracellular Ca2Aortic endothelial cellsKinase 1Bovine aortic endothelial cellsStrain-induced activationCycles/minChannel blockersExtracellular signal-regulated kinases 1Signal-regulated kinases 1Calcium-independent activationBenzohydroquinoneERK1/2 activationERK1/2Free mediumEffect of Ca2ActivationCa2EGTA