2018
Regulation of Yes-Associated Protein by Laminar Flow
Chitragari G, Shalaby SY, Sumpio BJ, Kurita J, Sumpio BE. Regulation of Yes-Associated Protein by Laminar Flow. Annals Of Vascular Surgery 2018, 52: 183-191. PMID: 29758328, DOI: 10.1016/j.avsg.2018.03.002.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingCell ShapeCells, CulturedCytoskeletonDown-RegulationHuman Umbilical Vein Endothelial CellsHumansMechanotransduction, CellularPhosphoproteinsPhosphorylationProteolysisPulsatile FlowRegional Blood FlowStress, MechanicalTime FactorsTranscription FactorsYAP-Signaling ProteinsConceptsHuman umbilical vein endothelial cellsTotal YAPExposure of HUVECsRelease of mediatorsYAP levelsSignificant decreaseUbiquitin-proteasome system inhibitorsUmbilical vein endothelial cellsEndothelial cell proliferationVein endothelial cellsVascular healthSystem inhibitorsYes-Associated ProteinDisease modulationIntimal hyperplasiaDisturbed flowEndothelial cellsBlood vesselsDisease statesPotential targetCell proliferationProteasome-independent mechanismExposureKey regulatorPYAP
2017
Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow
Shalaby SY, Chitragari G, Sumpio BJ, Sumpio BE. Shear Stress Induces Change in Extracellular Signal-Regulated Kinase 5 Levels with Sustained Activation under Disturbed and Continuous Laminar Flow. International Journal Of Angiology 2017, 26: 109-115. PMID: 28566937, PMCID: PMC5446257, DOI: 10.1055/s-0037-1599057.Peer-Reviewed Original ResearchExtracellular signal-regulated kinase 5Pathogenesis of atherosclerosisExposure of HUVECERK5 activationVascular dysfunctionPattern of activationFro flowVascular remodelingConfluent HUVECsHours of exposurePhospho-ERK5Endothelial integrityActivation of ERK5Kinase 5Sustained activationEnd of experimentationERK5 phosphorylationHUVECActivationHoursExposureTransient phosphorylationCell lysatesDisturbed flow conditionsParallel plate flow chamber