2008
The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival
Nishimura K, Blume P, Ohgi S, Sumpio BE. The Effect of Different Frequencies of Stretch on Human Dermal Keratinocyte Proliferation and Survival. Journal Of Surgical Research 2008, 155: 125-131. PMID: 19059608, DOI: 10.1016/j.jss.2008.07.029.Peer-Reviewed Original ResearchConceptsERK 1/2P38 MAPKProtein kinaseKeratinocyte proliferation rateCell deathAkt inhibitorCyclic stretchDNA synthesisAktTransferase-mediated dUTP nick-end labeling stainingRepetitive stretchApoptosisMAPKDUTP nick end labeling stainingProliferation rateHuman dermalCell numberNick end labeling stainingTUNEL-positive cellsDermal keratinocytesStretchTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingBrdU stainingKeratinocyte proliferationProliferation
2001
Cyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells
Smith JD, Davies N, Willis AI, Sumpio BE, Zilla P. Cyclic Stretch Induces the Expression of Vascular Endothelial Growth Factor in Vascular Smooth Muscle Cells. Endothelium 2001, 8: 41-48. PMID: 11409850, DOI: 10.3109/10623320109063156.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicCattleCells, CulturedCoculture TechniquesCulture MediaEndothelial Growth FactorsEndothelium, VascularGene Expression RegulationLymphokinesMuscle, Smooth, VascularPhosphorylationReceptor Protein-Tyrosine KinasesReceptors, Growth FactorReceptors, Vascular Endothelial Growth FactorStress, MechanicalVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVasodilationConceptsVascular smooth muscle cellsVascular endothelial growth factorSmooth muscle cellsEndothelial growth factorExposure of VSMCsMuscle cellsGrowth factorMigration of ECsBovine vascular smooth muscle cellsSteady-state levelsCyclic stretchNormal endothelial cell functionEndothelial cell functionVEGF receptor KDRRelease of VEGFVEGF receptor phosphorylationVEGF secretionMRNA steady-state levelsVEGF receptorsReceptor KDRVEGF mRNACell functionReceptor phosphorylationSignificant increaseEC survival
1992
Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro.
Rosales OR, Sumpio BE. Protein kinase C is a mediator of the adaptation of vascular endothelial cells to cyclic strain in vitro. Surgery 1992, 112: 459-66. PMID: 1379381.Peer-Reviewed Original ResearchConceptsProtein kinase CActivation of PKCImmunocytochemical stainingPKC activityCyclic stretchEndothelial cell activationVascular endothelial cellsCycles/minSustained elevationCell activationFlexible-bottomed culture platesKinase CBovine aortic ECsECS resultsEndothelial cellsBlood vesselsAortic ECsEvidence of translocationSpecific PKC inhibitorEC proliferationCalphostin CEC growthHemodynamic forces