1998
The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro
Sawmiller C, Powell R, Quader M, Dudrick S, Sumpio B. The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro. Journal Of Vascular Surgery 1998, 27: 1128-1140. PMID: 9652475, DOI: 10.1016/s0741-5214(98)70015-1.Peer-Reviewed Original ResearchConceptsNonionic contrastEndothelial cellsIonic contrastSmooth muscle cell proliferationSignificant decreaseSmooth muscle cell growthNonionic contrast agentsCultured bovine aorta endothelial cellsMuscle cell proliferationBrief exposureMuscle cell growthRole of osmolalityContrast exposureEC injuryContrast agentsAorta endothelial cellsLength of exposureDay 3SMC proliferationOsmolar effectsBovine aorta endothelial cellsEffect of contrastCell proliferationConfluent endothelial cellsOsmolar solution
1997
Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools
ROSALES O, ISALES C, BARRETT P, BROPHY C, SUMPIO B. Exposure of endothelial cells to cyclic strain induces elevations of cytosolic Ca2+ concentration through mobilization of intracellular and extracellular pools. Biochemical Journal 1997, 326: 385-392. PMID: 9291109, PMCID: PMC1218682, DOI: 10.1042/bj3260385.Peer-Reviewed Original ResearchConceptsMicroM GdCl3Endothelial cellsIP3 formationLate-phase componentsInitiation of stretchExtracellular poolConfluent bovine aortic endothelial cellsMobilization of intracellularAortic endothelial cellsBovine aortic endothelial cellsNM charybdotoxinCycles/minIP3 generationIntracellular Ca2Sustained phaseCytosolic Ca2Confluent endothelial cellsBenzohydroquinoneFree mediumTemporal relationshipMicroM NiCl2Ca2PreincubationCellsInitial rise